6qk4 - Revision history

OCA at 12:02, 24 January 2024

OCA — Wed, 24 Jan 2024 12:02:35 GMT

←Older revision		Revision as of 12:02, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6qk4' size='340' side='right'caption='[[6qk4]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='6qk4' size='340' side='right'caption='[[6qk4]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qk4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QK4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qk4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QK4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qk4 OCA], [~~http~~://pdbe.org/6qk4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qk4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qk4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qk4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.73Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qk4 OCA], [https://pdbe.org/6qk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qk4 RCSB], [https://www.ebi.ac.uk/pdbsum/6qk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qk4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q63QH7_BURPS Q63QH7_BURPS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Large Structures]]
-	[[Category: Allcock, N]]
-	[[Category: Auty, J M]]
-	[[Category: Barnes, K B]]
-	[[Category: Galyov, E E]]
-	[[Category: Harding, S V]]
-	[[Category: Jenkins, C H]]
-	[[Category: Mukamolova, G V]]
-	[[Category: Richards, M I]]
-	[[Category: Wallis, R]]
-	[[Category: Bacterial pathogenesis]]
	[[Category: Burkholderia pseudomallei]]		[[Category: Burkholderia pseudomallei]]
-	[[Category: ~~Cell division~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Allcock N]]
-	[[Category: ~~Lytic transglycosylase~~]]	+	[[Category: Auty JM]]
-	[[Category: ~~Peptidoglycan~~]]	+	[[Category: Barnes KB]]
		+	[[Category: Galyov EE]]
		+	[[Category: Harding SV]]
		+	[[Category: Jenkins CH]]
		+	[[Category: Mukamolova GV]]
		+	[[Category: Richards MI]]
		+	[[Category: Wallis R]]

OCA at 16:44, 14 August 2019

OCA — Wed, 14 Aug 2019 16:44:22 GMT

←Older revision		Revision as of 16:44, 14 August 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qk4 is ~~ON HOLD until Paper~~ Publication	+	==Lytic transglycosylase, LtgG, of Burkholderia pseudomallei.==
		+	<StructureSection load='6qk4' size='340' side='right'caption='[[6qk4]], [[Resolution\|resolution]] 1.73Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qk4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QK4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qk4 OCA], [http://pdbe.org/6qk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qk4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qk4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Burkholderia pseudomallei is the causative agent of the tropical disease melioidosis. Its genome encodes an arsenal of virulence factors that allow it, when required, to switch from a soil dwelling bacterium to a deadly intracellular pathogen. With a high intrinsic resistance to antibiotics and the ability to overcome challenges from the host immune system, there is an increasing requirement for new antibiotics and a greater understanding into the molecular mechanisms of B. pseudomallei virulence and dormancy. The peptidoglycan remodeling enzymes, lytic transglycosylases (Ltgs) are potential targets for such new antibiotics. Ltgs cleave the glycosidic bonds within bacterial peptidoglycan allowing for the insertion of peptidoglycan precursors during cell growth and division, and cell membrane spanning structures such as flagella and secretion systems. Using bioinformatic analysis we have identified 8 putative Ltgs in B. pseudomallei K96243. We aimed to investigate one of these Ltgs, LtgG (BPSL3046) through the generation of deletion mutants and biochemical analysis. We have shown that LtgG is a key contributor to cellular morphology, division, motility and virulence in BALB/c mice. We have determined the crystal structure of LtgG and have identified various amino acids likely to be important in peptidoglycan binding and catalytic activity. Recombinant protein assays and complementation studies using LtgG containing a site directed mutation in aspartate 343, confirmed the essentiality of this amino acid in the function of LtgG.

-	~~Authors: Jenkins~~, ~~C.H~~., ~~Wallis~~, R., Allcock, N., Barnes~~, K.B.~~, Richards~~, M.I.~~, Auty~~, J.M.~~, Galyov~~, E.E.~~, Harding, S.V.~~, Mukamolova, G~~.V.	+	The lytic transglycosylase, LtgG, controls cell morphology and virulence in Burkholderia pseudomallei.,Jenkins CH, Wallis R, Allcock N, Barnes KB, Richards MI, Auty JM, Galyov EE, Harding SV, Mukamolova GV Sci Rep. 2019 Jul 30;9(1):11060. doi: 10.1038/s41598-019-47483-z. PMID:31363151<ref>PMID:31363151</ref>

-	~~Description: Lytic transglycosylase, LtgG~~, of ~~Burkholderia pseudomallei~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Auty, J.M]]~~	+	<div class="pdbe-citations 6qk4" style="background-color:#fffaf0;"></div>
-	~~[[Category: Barnes, K.B]]~~	+	== References ==
-	[[Category: ~~Jenkins, C.H~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Allcock, N]]		[[Category: Allcock, N]]
-	[[Category: ~~Richards~~, M.I]]	+	[[Category: Auty, J M]]
-	[[Category: Harding, S.V]]	+	[[Category: Barnes, K B]]
		+	[[Category: Galyov, E E]]
		+	[[Category: Harding, S V]]
		+	[[Category: Jenkins, C H]]
		+	[[Category: Mukamolova, G V]]
		+	[[Category: Richards, M I]]
	[[Category: Wallis, R]]		[[Category: Wallis, R]]
-	[[Category: ~~Mukamolova, G.V~~]]	+	[[Category: Bacterial pathogenesis]]
-	[[Category: ~~Galyov, E.E~~]]	+	[[Category: Burkholderia pseudomallei]]
		+	[[Category: Cell division]]
		+	[[Category: Lyase]]
		+	[[Category: Lytic transglycosylase]]
		+	[[Category: Peptidoglycan]]

OCA: Protected "6qk4" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2019 07:58:06 GMT

Protected "6qk4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:58, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6qk4 is ON HOLD until Paper Publication Authors: Jenkins, C.H., Wallis, R., Allcock, N., Barnes, K.B., Richards, M.I., Auty, J.M., Galyov, E.E., Har...

OCA — Wed, 13 Feb 2019 07:58:04 GMT

New page: '''Unreleased structure''' The entry 6qk4 is ON HOLD until Paper Publication Authors: Jenkins, C.H., Wallis, R., Allcock, N., Barnes, K.B., Richards, M.I., Auty, J.M., Galyov, E.E., Har...

New page

'''Unreleased structure'''

The entry 6qk4 is ON HOLD until Paper Publication

Authors: Jenkins, C.H., Wallis, R., Allcock, N., Barnes, K.B., Richards, M.I., Auty, J.M., Galyov, E.E., Harding, S.V., Mukamolova, G.V.

Description: Lytic transglycosylase, LtgG, of Burkholderia pseudomallei.
[[Category: Unreleased Structures]]
[[Category: Auty, J.M]]
[[Category: Barnes, K.B]]
[[Category: Jenkins, C.H]]
[[Category: Allcock, N]]
[[Category: Richards, M.I]]
[[Category: Harding, S.V]]
[[Category: Wallis, R]]
[[Category: Mukamolova, G.V]]
[[Category: Galyov, E.E]]