6qm4 - Revision history

OCA at 05:02, 11 April 2020

OCA — Sat, 11 Apr 2020 05:02:24 GMT

←Older revision		Revision as of 05:02, 11 April 2020
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	<SX load='6qm4' size='340' side='right' viewer='molstar' caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='6qm4' size='340' side='right' viewer='molstar' caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qm4 OCA], [http://pdbe.org/6qm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qm4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qm4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qm4 OCA], [http://pdbe.org/6qm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qm4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qm4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:51, 6 March 2020

OCA — Fri, 06 Mar 2020 23:51:24 GMT

←Older revision		Revision as of 23:51, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc==		==Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc==
-	<~~StructureSection~~ load='6qm4' size='340' side='right'caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<SX load='6qm4' size='340' side='right' viewer='molstar' caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Fusarium solani subsp. pisi]]		[[Category: Fusarium solani subsp. pisi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 12:43, 18 December 2019

OCA — Wed, 18 Dec 2019 12:43:43 GMT

←Older revision		Revision as of 12:43, 18 December 2019
Line 1:		Line 1:

	==Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc==		==Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc==
-	<StructureSection load='6qm4' size='340' side='right' caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='6qm4' size='340' side='right'caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qm4 OCA], [http://pdbe.org/6qm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qm4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qm4 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qm4 OCA], [http://pdbe.org/6qm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qm4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qm4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium solani subsp. pisi]]
		+	[[Category: Large Structures]]
	[[Category: Bryner, L]]		[[Category: Bryner, L]]
	[[Category: Dutzler, R]]		[[Category: Dutzler, R]]

OCA at 07:38, 6 March 2019

OCA — Wed, 06 Mar 2019 07:38:17 GMT

←Older revision		Revision as of 07:38, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qm4 is ~~ON HOLD~~	+	==Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc==
		+	<StructureSection load='6qm4' size='340' side='right' caption='[[6qm4]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qm4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QM4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qm4 OCA], [http://pdbe.org/6qm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qm4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qm4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Scramblases catalyze the movement of lipids between both leaflets of a bilayer. Whereas the X-ray structure of the protein nhTMEM16 has previously revealed the architecture of a Ca(2+)-dependent lipid scramblase, its regulation mechanism has remained elusive. Here, we have used cryo-electron microscopy and functional assays to address this question. Ca(2+)-bound and Ca(2+)-free conformations of nhTMEM16 in detergent and lipid nanodiscs illustrate the interactions with its environment and they reveal the conformational changes underlying its activation. In this process, Ca(2+)-binding induces a stepwise transition of the catalytic subunit cavity, converting a closed cavity that is shielded from the membrane in the absence of ligand, into a polar furrow that becomes accessible to lipid headgroups in the Ca(2+)-bound state. Additionally, our structures demonstrate how nhTMEM16 distorts the membrane at both entrances of the subunit cavity, thereby decreasing the energy barrier for lipid movement.

-	~~Authors: Kalienkova~~, V., Clerico Mosina, V., Bryner, L., Oostergetel~~, G.T.~~, Dutzler, R., Paulino, C.	+	Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM.,Kalienkova V, Clerico Mosina V, Bryner L, Oostergetel GT, Dutzler R, Paulino C Elife. 2019 Feb 20;8. pii: 44364. doi: 10.7554/eLife.44364. PMID:30785398<ref>PMID:30785398</ref>

-	~~Description: Cryo-EM structure~~ of ~~calcium-free nhTMEM16 lipid scramblase in nanodisc~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Kalienkova, V]]~~	+	<div class="pdbe-citations 6qm4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bryner, L]]		[[Category: Bryner, L]]
-	[[Category: Paulino, C]]
	[[Category: Dutzler, R]]		[[Category: Dutzler, R]]
-	[[Category: ~~Clerico~~ Mosina, V]]	+	[[Category: Kalienkova, V]]
-	[[Category: Oostergetel, G.T]]	+	[[Category: Mosina, V Clerico]]
		+	[[Category: Oostergetel, G T]]
		+	[[Category: Paulino, C]]
		+	[[Category: Lipid scramble]]
		+	[[Category: Membrane protein]]
		+	[[Category: Tmem16]]

OCA: Protected "6qm4" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2019 08:05:22 GMT

Protected "6qm4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:05, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6qm4 is ON HOLD Authors: Kalienkova, V., Clerico Mosina, V., Bryner, L., Oostergetel, G.T., Dutzler, R., Paulino, C. Description: Cryo-EM structure...

OCA — Wed, 13 Feb 2019 08:05:21 GMT

New page: '''Unreleased structure''' The entry 6qm4 is ON HOLD Authors: Kalienkova, V., Clerico Mosina, V., Bryner, L., Oostergetel, G.T., Dutzler, R., Paulino, C. Description: Cryo-EM structure...

New page

'''Unreleased structure'''

The entry 6qm4 is ON HOLD

Authors: Kalienkova, V., Clerico Mosina, V., Bryner, L., Oostergetel, G.T., Dutzler, R., Paulino, C.

Description: Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc
[[Category: Unreleased Structures]]
[[Category: Kalienkova, V]]
[[Category: Bryner, L]]
[[Category: Paulino, C]]
[[Category: Dutzler, R]]
[[Category: Clerico Mosina, V]]
[[Category: Oostergetel, G.T]]