6qpb - Revision history

OCA at 05:04, 11 April 2020

OCA — Sat, 11 Apr 2020 05:04:57 GMT

←Older revision		Revision as of 05:04, 11 April 2020
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	<SX load='6qpb' size='340' side='right' viewer='molstar' caption='[[6qpb]], [[Resolution\|resolution]] 3.60Å' scene=''>		<SX load='6qpb' size='340' side='right' viewer='molstar' caption='[[6qpb]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QPB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QPB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpb OCA], [http://pdbe.org/6qpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpb RCSB], [http://www.ebi.ac.uk/pdbsum/6qpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpb OCA], [http://pdbe.org/6qpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpb RCSB], [http://www.ebi.ac.uk/pdbsum/6qpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:52, 6 March 2020

OCA — Fri, 06 Mar 2020 23:52:28 GMT

←Older revision		Revision as of 23:52, 6 March 2020
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	==Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin==		==Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin==
-	<~~StructureSection~~ load='6qpb' size='340' side='right'caption='[[6qpb]], [[Resolution\|resolution]] 3.60Å' scene=''>	+	<SX load='6qpb' size='340' side='right' viewer='molstar' caption='[[6qpb]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPB FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPB FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]

OCA at 08:33, 20 March 2019

OCA — Wed, 20 Mar 2019 08:33:53 GMT

←Older revision		Revision as of 08:33, 20 March 2019
Line 1:		Line 1:

	==Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin==		==Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin==
-	<StructureSection load='6qpb' size='340' side='right' caption='[[6qpb]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='6qpb' size='340' side='right'caption='[[6qpb]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpb OCA], [http://pdbe.org/6qpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpb RCSB], [http://www.ebi.ac.uk/pdbsum/6qpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpb OCA], [http://pdbe.org/6qpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpb RCSB], [http://www.ebi.ac.uk/pdbsum/6qpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpb ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Alvadia, C]]		[[Category: Alvadia, C]]
	[[Category: Dutzler, R]]		[[Category: Dutzler, R]]

OCA at 07:38, 6 March 2019

OCA — Wed, 06 Mar 2019 07:38:52 GMT

←Older revision		Revision as of 07:38, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qpb is ~~ON HOLD~~	+	==Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin==
		+	<StructureSection load='6qpb' size='340' side='right' caption='[[6qpb]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qpb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpb OCA], [http://pdbe.org/6qpb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpb RCSB], [http://www.ebi.ac.uk/pdbsum/6qpb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ANO6_MOUSE ANO6_MOUSE]] Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.<ref>PMID:21107324</ref> <ref>PMID:21908539</ref> <ref>PMID:22936354</ref> <ref>PMID:23021219</ref> <ref>PMID:23532839</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lipid scramblase TMEM16F initiates blood coagulation by catalyzing the exposure of phosphatidylserine in platelets. The protein is part of a family of membrane proteins, which encompasses calcium-activated channels for ions and lipids. Here, we reveal features of murine TMEM16F (mTMEM16F) that underlie its function as a lipid scramblase and an ion channel. The cryo-EM data of mTMEM16F in absence and presence of Ca(2+) define the ligand-free closed conformation of the protein and the structure of a Ca(2+)-bound intermediate. Both conformations resemble their counterparts of the scrambling-incompetent anion channel mTMEM16A, yet with distinct differences in the region of ion and lipid permeation. In conjunction with functional data, we demonstrate the relationship between ion conduction and lipid scrambling. Although activated by a common mechanism, both functions appear to be mediated by alternate protein conformations that are at equilibrium in the ligand-bound state.

-	~~Authors: Alvadia~~, C., Lim~~, N.K.~~, Clerico Mosina, V., Oostergetel~~, G.T.~~, Dutzler, R., Paulino, C.	+	Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F.,Alvadia C, Lim NK, Clerico Mosina V, Oostergetel GT, Dutzler R, Paulino C Elife. 2019 Feb 20;8. pii: 44365. doi: 10.7554/eLife.44365. PMID:30785399<ref>PMID:30785399</ref>

-	~~Description: Cryo-EM structure~~ of ~~calcium-free mTMEM16F lipid scramblase in digitonin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Lim, N.K]]~~	+	<div class="pdbe-citations 6qpb" style="background-color:#fffaf0;"></div>
-	~~[[Category: Paulino, C]]~~	+	== References ==
-	~~[[Category: Dutzler, R]]~~	+	<references/>
-	~~[[Category: Clerico Mosina, V]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Alvadia, C]]		[[Category: Alvadia, C]]
-	[[Category: Oostergetel, G.T]]	+	[[Category: Dutzler, R]]
		+	[[Category: Lim, N K]]
		+	[[Category: Mosina, V Clerico]]
		+	[[Category: Oostergetel, G T]]
		+	[[Category: Paulino, C]]
		+	[[Category: Lipid scramble]]
		+	[[Category: Membrane protein]]
		+	[[Category: Tmem16]]

OCA at 13:54, 27 February 2019

OCA — Wed, 27 Feb 2019 13:54:13 GMT

←Older revision		Revision as of 13:54, 27 February 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qpb is ON HOLD ~~until sometime in the future~~	+	The entry 6qpb is ON HOLD

	Authors: Alvadia, C., Lim, N.K., Clerico Mosina, V., Oostergetel, G.T., Dutzler, R., Paulino, C.		Authors: Alvadia, C., Lim, N.K., Clerico Mosina, V., Oostergetel, G.T., Dutzler, R., Paulino, C.

OCA: Protected "6qpb" [edit=sysop:move=sysop]

OCA — Thu, 21 Feb 2019 05:26:44 GMT

Protected "6qpb" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 21 February 2019

OCA: New page: '''Unreleased structure''' The entry 6qpb is ON HOLD until sometime in the future Authors: Alvadia, C., Lim, N.K., Clerico Mosina, V., Oostergetel, G.T., Dutzler, R., Paulino, C. Descr...

OCA — Thu, 21 Feb 2019 05:26:42 GMT

New page: '''Unreleased structure''' The entry 6qpb is ON HOLD until sometime in the future Authors: Alvadia, C., Lim, N.K., Clerico Mosina, V., Oostergetel, G.T., Dutzler, R., Paulino, C. Descr...

New page

'''Unreleased structure'''

The entry 6qpb is ON HOLD until sometime in the future

Authors: Alvadia, C., Lim, N.K., Clerico Mosina, V., Oostergetel, G.T., Dutzler, R., Paulino, C.

Description: Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin
[[Category: Unreleased Structures]]
[[Category: Lim, N.K]]
[[Category: Paulino, C]]
[[Category: Dutzler, R]]
[[Category: Clerico Mosina, V]]
[[Category: Alvadia, C]]
[[Category: Oostergetel, G.T]]