6qpv - Revision history

OCA at 12:05, 24 January 2024

OCA — Wed, 24 Jan 2024 12:05:58 GMT

←Older revision		Revision as of 12:05, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6qpv' size='340' side='right'caption='[[6qpv]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='6qpv' size='340' side='right'caption='[[6qpv]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qpv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_27511 Atcc 27511~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qpv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_pickettii Ralstonia pickettii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QPV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CU:COPPER+(II)+ION'>CU</scene~~>, ~~<scene name='pdbligand=HEC:HEME+C'>HEC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>HMPREF0989_00586 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~329 ATCC 27511])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpv OCA], [https://pdbe.org/6qpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qpv RCSB], [https://www.ebi.ac.uk/pdbsum/6qpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpv ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpv OCA], [~~http~~://pdbe.org/6qpv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qpv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qpv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpv ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/I6NAW4_RALPI I6NAW4_RALPI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 27511]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Antonyuk, S V~~]]	+	[[Category: Ralstonia pickettii]]
-	[[Category: ~~Eady, R R~~]]	+	[[Category: Antonyuk SV]]
-	[[Category: ~~Hasnain, S S~~]]	+	[[Category: Eady RR]]
-	[[Category: ~~Hedison, T M~~]]	+	[[Category: Hasnain SS]]
-	[[Category: ~~Scrutton, N S~~]]	+	[[Category: Hedison TM]]
-	[[Category: ~~Shenoy, R T~~]]	+	[[Category: Scrutton NS]]
-	[[Category: ~~Haem and cu containing nitrite reductase]]~~	+	[[Category: Shenoy RT]]
-	~~[[Category: Inter-copper electron transfer]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 10:28, 26 February 2020

OCA — Wed, 26 Feb 2020 10:28:06 GMT

←Older revision		Revision as of 10:28, 26 February 2020
Line 3:		Line 3:
	<StructureSection load='6qpv' size='340' side='right'caption='[[6qpv]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='6qpv' size='340' side='right'caption='[[6qpv]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qpv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qpv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27511 Atcc 27511]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF0989_00586 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=329 ATCC 27511])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpv OCA], [http://pdbe.org/6qpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpv RCSB], [http://www.ebi.ac.uk/pdbsum/6qpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpv OCA], [http://pdbe.org/6qpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpv RCSB], [http://www.ebi.ac.uk/pdbsum/6qpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It is generally assumed that tethering enhances rates of electron harvesting and delivery to active sites in multidomain enzymes by proximity and sampling mechanisms. Here, we explore this idea in a tethered 3-domain, trimeric copper-containing nitrite reductase. By reverse engineering, we find that tethering does not enhance the rate of electron delivery from its pendant cytochrome c to the catalytic copper-containing core. Using a linker that harbors a gatekeeper tyrosine in a nitrite access channel, the tethered haem domain enables catalysis by other mechanisms. Tethering communicates the redox state of the haem to the distant T2Cu center that helps initiate substrate binding for catalysis. It also tunes copper reduction potentials, suppresses reductive enzyme inactivation, enhances enzyme affinity for substrate, and promotes intercopper electron transfer. Tethering has multiple unanticipated beneficial roles, the combination of which fine-tunes function beyond simplistic mechanisms expected from proximity and restrictive sampling models.
		+
		+	Unexpected Roles of a Tether Harboring a Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis.,Hedison TM, Shenoy RT, Iorgu AI, Heyes DJ, Fisher K, Wright GSA, Hay S, Eady RR, Antonyuk SV, Hasnain SS, Scrutton NS ACS Catal. 2019 Jul 5;9(7):6087-6099. doi: 10.1021/acscatal.9b01266. Epub 2019, May 29. PMID:32051772<ref>PMID:32051772</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qpv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitrite reductase 3D structures\|Nitrite reductase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 27511]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Antonyuk, S V]]		[[Category: Antonyuk, S V]]

OCA at 06:48, 6 November 2019

OCA — Wed, 06 Nov 2019 06:48:49 GMT

←Older revision		Revision as of 06:48, 6 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qpv ~~is ON HOLD~~	+	==Crystal structure of as isolated Y323A mutant of haem-Cu containing nitrite reductase from Ralstonia pickettii==
-		+	<StructureSection load='6qpv' size='340' side='right'caption='[[6qpv]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~Authors~~: ~~Antonyuk~~, S.V., ~~Shenoy, R~~.T., ~~Hedison, T~~.M., ~~Eady~~, R.R., ~~Hasnain, S~~.S., ~~Scrutton, N~~.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qpv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QPV FirstGlance]. <br>
-	~~Description: Crystal structure of as isolated Y323A mutant of haem-Cu containing nitrite reductase from Ralstonia pickettii~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span></td></tr>
-	[[Category: Eady, R.R]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qpv OCA], [http://pdbe.org/6qpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qpv RCSB], [http://www.ebi.ac.uk/pdbsum/6qpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qpv ProSAT]</span></td></tr>
-	[[Category: ~~Hedison~~, ~~T.M~~]]	+	</table>
-	[[Category: ~~Shenoy~~, R.T]]	+	__TOC__
-	[[Category: Scrutton, N.S]]	+	</StructureSection>
-	[[Category: ~~Hasnain~~, ~~S.S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Antonyuk, S.V~~]]	+	[[Category: Antonyuk, S V]]
		+	[[Category: Eady, R R]]
		+	[[Category: Hasnain, S S]]
		+	[[Category: Hedison, T M]]
		+	[[Category: Scrutton, N S]]
		+	[[Category: Shenoy, R T]]
		+	[[Category: Haem and cu containing nitrite reductase]]
		+	[[Category: Inter-copper electron transfer]]
		+	[[Category: Metal binding protein]]

OCA: Protected "6qpv" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2019 13:54:53 GMT

Protected "6qpv" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:54, 27 February 2019

OCA: New page: '''Unreleased structure''' The entry 6qpv is ON HOLD Authors: Antonyuk, S.V., Shenoy, R.T., Hedison, T.M., Eady, R.R., Hasnain, S.S., Scrutton, N.S. Description: Crystal structure of a...

OCA — Wed, 27 Feb 2019 13:54:52 GMT

New page: '''Unreleased structure''' The entry 6qpv is ON HOLD Authors: Antonyuk, S.V., Shenoy, R.T., Hedison, T.M., Eady, R.R., Hasnain, S.S., Scrutton, N.S. Description: Crystal structure of a...

New page

'''Unreleased structure'''

The entry 6qpv is ON HOLD

Authors: Antonyuk, S.V., Shenoy, R.T., Hedison, T.M., Eady, R.R., Hasnain, S.S., Scrutton, N.S.

Description: Crystal structure of as isolated Y323A mutant of haem-Cu containing nitrite reductase from Ralstonia pickettii
[[Category: Unreleased Structures]]
[[Category: Eady, R.R]]
[[Category: Hedison, T.M]]
[[Category: Shenoy, R.T]]
[[Category: Scrutton, N.S]]
[[Category: Hasnain, S.S]]
[[Category: Antonyuk, S.V]]