6qtn - Revision history

OCA at 12:08, 24 January 2024

OCA — Wed, 24 Jan 2024 12:08:25 GMT

←Older revision		Revision as of 12:08, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6qtn' size='340' side='right'caption='[[6qtn]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6qtn' size='340' side='right'caption='[[6qtn]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qtn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~] and [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QTN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qtn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QTN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=JHH:Cyclostreptin'>JHH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Stmn4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), TTL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=JHH:Cyclostreptin'>JHH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qtn OCA], [https://pdbe.org/6qtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qtn RCSB], [https://www.ebi.ac.uk/pdbsum/6qtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qtn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qtn OCA], [https://pdbe.org/6qtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qtn RCSB], [https://www.ebi.ac.uk/pdbsum/6qtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qtn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: ~~Buffalo rat]]~~	+	[[Category: Gallus gallus]]
-	~~[[Category: Chick~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Altmann, K H]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Balaguer~~, F d.A~~]]	+	[[Category: Altmann K-H]]
-	[[Category: Calvo, E]]	+	[[Category: Balaguer FdA]]
-	[[Category: Diaz~~, J F~~]]	+	[[Category: Calvo E]]
-	[[Category: Estevez-Gallego, J]]	+	[[Category: Diaz JF]]
-	[[Category: Gimenez-Abian~~, J F~~]]	+	[[Category: Estevez-Gallego J]]
-	[[Category: Mooberry~~, S L~~]]	+	[[Category: Gimenez-Abian JF]]
-	[[Category: Muehlethaler, T]]	+	[[Category: Mooberry SL]]
-	[[Category: Oliva~~, M A~~]]	+	[[Category: Muehlethaler T]]
-	[[Category: Prota~~, A E~~]]	+	[[Category: Oliva MA]]
-	[[Category: Risinger~~, A L~~]]	+	[[Category: Prota AE]]
-	[[Category: Sorensen~~, E J~~]]	+	[[Category: Risinger AL]]
-	[[Category: Steinmetz~~, M O~~]]	+	[[Category: Sorensen EJ]]
-	[[Category: Vanderwal~~, C D]]~~	+	[[Category: Steinmetz MO]]
-	~~[[Category: Cell cycle]]~~	+	[[Category: Vanderwal CD]]
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Tubulin fold~~]]	+

OCA at 11:05, 30 March 2022

OCA — Wed, 30 Mar 2022 11:05:28 GMT

←Older revision		Revision as of 11:05, 30 March 2022
Line 3:		Line 3:
	<StructureSection load='6qtn' size='340' side='right'caption='[[6qtn]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6qtn' size='340' side='right'caption='[[6qtn]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qtn]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/ ] and [~~http~~://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QTN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QTN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qtn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QTN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=JHH:Cyclostreptin'>JHH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=JHH:Cyclostreptin'>JHH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qtn OCA], [~~http~~://pdbe.org/6qtn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qtn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qtn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qtn ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Stmn4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), TTL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qtn OCA], [https://pdbe.org/6qtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qtn RCSB], [https://www.ebi.ac.uk/pdbsum/6qtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qtn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[~~http~~://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[~~http~~://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).	+	[[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6qtn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qtn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Stathmin-4 3D structures\|Stathmin-4 3D structures]]
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Buffalo rat]]
		+	[[Category: Chick]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Altmann, K H]]		[[Category: Altmann, K H]]

OCA at 07:14, 3 April 2019

OCA — Wed, 03 Apr 2019 07:14:49 GMT

←Older revision		Revision as of 07:14, 3 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qtn is ~~ON HOLD~~	+	==Tubulin-cyclostreptin complex==
		+	<StructureSection load='6qtn' size='340' side='right'caption='[[6qtn]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qtn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QTN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QTN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=JHH:Cyclostreptin'>JHH</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qtn OCA], [http://pdbe.org/6qtn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qtn RCSB], [http://www.ebi.ac.uk/pdbsum/6qtn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qtn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It has been proposed that one of the mechanisms of taxane-site ligand-mediated tubulin activation is modulation of the structure of a switch element (the M-loop) from a disordered form in dimeric tubulin to a folded helical structure in microtubules. Here, we used covalent taxane-site ligands, including cyclostreptin, to gain further insight into this mechanism. The crystal structure of cyclostreptin-bound tubulin reveals covalent binding to betaHis229, but no stabilization of the M-loop. The capacity of cyclostreptin to induce microtubule assembly compared to other covalent taxane-site agents demonstrates that the induction of tubulin assembly is not strictly dependent on M-loop stabilization. We further demonstrate that most covalent taxane-site ligands are able to partially overcome drug resistance mediated by betaIII-tubulin (betaIII) overexpression in HeLa cells, and compare their activities to pironetin, an interfacial covalent inhibitor of tubulin assembly that displays invariant growth inhibition in these cells. Our findings suggest a relationship between a diminished interaction of taxane-site ligands with betaIII-tubulin and betaIII tubulin-mediated drug resistance. This supports the idea that overexpression of betaIII increases microtubule dynamicity by counteracting the enhanced microtubule stability promoted by covalent taxane-site binding ligands.

-	~~Authors~~:	+	Crystal Structure of the Cyclostreptin-Tubulin Adduct: Implications for Tubulin Activation by Taxane-Site Ligands.,Balaguer FA, Muhlethaler T, Estevez-Gallego J, Calvo E, Gimenez-Abian JF, Risinger AL, Sorensen EJ, Vanderwal CD, Altmann KH, Mooberry SL, Steinmetz MO, Oliva MA, Prota AE, Diaz JF Int J Mol Sci. 2019 Mar 20;20(6). pii: ijms20061392. doi: 10.3390/ijms20061392. PMID:30897704<ref>PMID:30897704</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6qtn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
		+	[[Category: Altmann, K H]]
		+	[[Category: Balaguer, F d.A]]
		+	[[Category: Calvo, E]]
		+	[[Category: Diaz, J F]]
		+	[[Category: Estevez-Gallego, J]]
		+	[[Category: Gimenez-Abian, J F]]
		+	[[Category: Mooberry, S L]]
		+	[[Category: Muehlethaler, T]]
		+	[[Category: Oliva, M A]]
		+	[[Category: Prota, A E]]
		+	[[Category: Risinger, A L]]
		+	[[Category: Sorensen, E J]]
		+	[[Category: Steinmetz, M O]]
		+	[[Category: Vanderwal, C D]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cytoskeleton]]
		+	[[Category: Microtubule]]
		+	[[Category: Tubulin fold]]

OCA: Protected "6qtn" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2019 05:53:41 GMT

Protected "6qtn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:53, 6 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qtn is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Mar 2019 05:53:39 GMT

New page: '''Unreleased structure''' The entry 6qtn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qtn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]