6qv2 - Revision history

OCA at 23:14, 5 June 2019

OCA — Wed, 05 Jun 2019 23:14:20 GMT

←Older revision		Revision as of 23:14, 5 June 2019
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	<StructureSection load='6qv2' size='340' side='right'caption='[[6qv2]], [[Resolution\|resolution]] 4.23Å' scene=''>		<StructureSection load='6qv2' size='340' side='right'caption='[[6qv2]], [[Resolution\|resolution]] 4.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qv2]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QV2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qv2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Alpaca Alpaca] and [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QV2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0287 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA]), TM_0288 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qv2 OCA], [http://pdbe.org/6qv2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qv2 RCSB], [http://www.ebi.ac.uk/pdbsum/6qv2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qv2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qv2 OCA], [http://pdbe.org/6qv2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qv2 RCSB], [http://www.ebi.ac.uk/pdbsum/6qv2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qv2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ABC exporters harness the energy of ATP to pump substrates across membranes. Extracellular gate opening and closure are key steps of the transport cycle, but the underlying mechanism is poorly understood. Here, we generated a synthetic single domain antibody (sybody) that recognizes the heterodimeric ABC exporter TM287/288 exclusively in the presence of ATP, which was essential to solve a 3.2 A crystal structure of the outward-facing transporter. The sybody binds to an extracellular wing and strongly inhibits ATPase activity by shifting the transporter's conformational equilibrium towards the outward-facing state, as shown by double electron-electron resonance (DEER). Mutations that facilitate extracellular gate opening result in a comparable equilibrium shift and strongly reduce ATPase activity and drug transport. Using the sybody as conformational probe, we demonstrate that efficient extracellular gate closure is required to dissociate the NBD dimer after ATP hydrolysis to reset the transporter back to its inward-facing state.
		+
		+	The extracellular gate shapes the energy profile of an ABC exporter.,Hutter CAJ, Timachi MH, Hurlimann LM, Zimmermann I, Egloff P, Goddeke H, Kucher S, Stefanic S, Karttunen M, Schafer LV, Bordignon E, Seeger MA Nat Commun. 2019 May 21;10(1):2260. doi: 10.1038/s41467-019-09892-6. PMID:31113958<ref>PMID:31113958</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qv2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alpaca]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Thema]]
	[[Category: Egloff, P]]		[[Category: Egloff, P]]
	[[Category: Huerlimann, L M]]		[[Category: Huerlimann, L M]]

OCA at 06:21, 29 May 2019

OCA — Wed, 29 May 2019 06:21:33 GMT

←Older revision		Revision as of 06:21, 29 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6qv2 ~~is ON HOLD~~	+	==Structure of ATPgS-bound outward-facing TM287/288 in complex with nanobody Nb_TM#2==
-		+	<StructureSection load='6qv2' size='340' side='right'caption='[[6qv2]], [[Resolution\|resolution]] 4.23Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qv2]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QV2 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qv2 OCA], [http://pdbe.org/6qv2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qv2 RCSB], [http://www.ebi.ac.uk/pdbsum/6qv2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qv2 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Egloff, P]]
		+	[[Category: Huerlimann, L M]]
		+	[[Category: Hutter, C A.J]]
		+	[[Category: Seeger, M A]]
		+	[[Category: Zimmermann, I]]
		+	[[Category: Abc exporter]]
		+	[[Category: Abc transporter]]
		+	[[Category: Membrane protein]]
		+	[[Category: Membrane transporter]]
		+	[[Category: Nanobody]]

OCA: Protected "6qv2" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2019 05:58:31 GMT

Protected "6qv2" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:58, 6 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qv2 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Mar 2019 05:58:29 GMT

New page: '''Unreleased structure''' The entry 6qv2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qv2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]