6qve - Revision history

OCA at 05:09, 11 April 2020

OCA — Sat, 11 Apr 2020 05:09:05 GMT

←Older revision		Revision as of 05:09, 11 April 2020
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	<SX load='6qve' size='340' side='right' viewer='molstar' caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='6qve' size='340' side='right' viewer='molstar' caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Amoeba Amoeba] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QVE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Amoeba Amoeba] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QVE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6qus\|6qus]], [[6quy\|6quy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6qus\|6qus]], [[6quy\|6quy]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NAEGRDRAFT_50049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5762 Amoeba])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NAEGRDRAFT_50049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5762 Amoeba])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qve OCA], [http://pdbe.org/6qve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qve RCSB], [http://www.ebi.ac.uk/pdbsum/6qve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qve ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qve OCA], [http://pdbe.org/6qve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qve RCSB], [http://www.ebi.ac.uk/pdbsum/6qve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qve ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6qve" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qve" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:55, 6 March 2020

OCA — Fri, 06 Mar 2020 23:55:24 GMT

←Older revision		Revision as of 23:55, 6 March 2020
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	==NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule==		==NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule==
-	<~~StructureSection~~ load='6qve' size='340' side='right'caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<SX load='6qve' size='340' side='right' viewer='molstar' caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Amoeba Amoeba] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVE FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Amoeba Amoeba] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVE FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Amoeba]]		[[Category: Amoeba]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 10:53, 4 December 2019

OCA — Wed, 04 Dec 2019 10:53:16 GMT

←Older revision		Revision as of 10:53, 4 December 2019
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	<StructureSection load='6qve' size='340' side='right'caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='6qve' size='340' side='right'caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Amoeba Amoeba] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6qus\|6qus]], [[6quy\|6quy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6qus\|6qus]], [[6quy\|6quy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NAEGRDRAFT_50049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5762 Amoeba])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qve OCA], [http://pdbe.org/6qve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qve RCSB], [http://www.ebi.ac.uk/pdbsum/6qve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qve ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qve OCA], [http://pdbe.org/6qve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qve RCSB], [http://www.ebi.ac.uk/pdbsum/6qve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qve ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TBA1B_HUMAN TBA1B_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/A0A2K5HGL3_COLAP A0A2K5HGL3_COLAP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]		[[http://www.uniprot.org/uniprot/TBA1B_HUMAN TBA1B_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/A0A2K5HGL3_COLAP A0A2K5HGL3_COLAP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CAMSAP/Patronins regulate microtubule minus-end dynamics. Their end specificity is mediated by their CKK domains, which we proposed recognise specific tubulin conformations found at minus ends. To critically test this idea, we compared the human CAMSAP1 CKK domain (HsCKK) with a CKK domain from Naegleria gruberi (NgCKK), which lacks minus-end specificity. Here we report near-atomic cryo-electron microscopy structures of HsCKK- and NgCKK-microtubule complexes, which show that these CKK domains share the same protein fold, bind at the intradimer interprotofilament tubulin junction, but exhibit different footprints on microtubules. NMR experiments show that both HsCKK and NgCKK are remarkably rigid. However, whereas NgCKK binding does not alter the microtubule architecture, HsCKK remodels its microtubule interaction site and changes the underlying polymer structure because the tubulin lattice conformation is not optimal for its binding. Thus, in contrast to many MAPs, the HsCKK domain can differentiate subtly specific tubulin conformations to enable microtubule minus-end recognition.
		+
		+	Structural determinants of microtubule minus end preference in CAMSAP CKK domains.,Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA Nat Commun. 2019 Nov 20;10(1):5236. doi: 10.1038/s41467-019-13247-6. PMID:31748546<ref>PMID:31748546</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qve" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Amoeba]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:55, 27 November 2019

OCA — Wed, 27 Nov 2019 06:55:57 GMT

←Older revision		Revision as of 06:55, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qve ~~is ON HOLD~~	+	==NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule==
-		+	<StructureSection load='6qve' size='340' side='right'caption='[[6qve]], [[Resolution\|resolution]] 3.70Å' scene=''>
-	~~Authors~~: ~~Atherton, J~~.M.~~, Luo, Y~~.~~, Xiang, S~~.~~, Yang, C~~.~~, Jiang, K~~.~~, Stangier, M~~.~~, Vemu, A~~., ~~Cook~~, A., ~~Wang~~, S.~~, Roll~~-~~Mecak, A~~., ~~Steinmetz, M~~.O., ~~Akhmanova~~, A., ~~Baldus, M~~., ~~Moores, C~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qve]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QVE FirstGlance]. <br>
-	~~Description~~: ~~NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol~~-~~GDP microtubule~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6qus\|6qus]], [[6quy\|6quy]]</td></tr>
-	[[Category: ~~Vemu, A~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qve OCA], [http://pdbe.org/6qve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qve RCSB], [http://www.ebi.ac.uk/pdbsum/6qve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qve ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_HUMAN TBA1B_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/A0A2K5HGL3_COLAP A0A2K5HGL3_COLAP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Akhmanova, A]]		[[Category: Akhmanova, A]]
		+	[[Category: Atherton, J M]]
		+	[[Category: Baldus, M]]
		+	[[Category: Cook, A]]
		+	[[Category: Jiang, K]]
		+	[[Category: Luo, Y]]
		+	[[Category: Moores, C A]]
	[[Category: Roll-Mecak, A]]		[[Category: Roll-Mecak, A]]
	[[Category: Stangier, M]]		[[Category: Stangier, M]]
-	[[Category: ~~Luo~~, Y]]	+	[[Category: Steinmetz, M O]]
-	[[Category: ~~Cook~~, A]]	+	[[Category: Vemu, A]]
	[[Category: Wang, S]]		[[Category: Wang, S]]
-	[[Category: Moores, C.A]]
	[[Category: Xiang, S]]		[[Category: Xiang, S]]
-	[[Category: Baldus, M]]
-	[[Category: Steinmetz, M.O]]
	[[Category: Yang, C]]		[[Category: Yang, C]]
-	[[Category: ~~Jiang, K~~]]	+	[[Category: Microtubule camsap calmodulin-regulated spectrum-associated proteins ckk cryo-em cryo-electron microscopy]]
-	[[Category: ~~Atherton, J.M~~]]	+	[[Category: Structural protein]]

OCA: Protected "6qve" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2019 10:23:29 GMT

Protected "6qve" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:23, 13 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qve is ON HOLD Authors: Atherton, J.M., Luo, Y., Xiang, S., Yang, C., Jiang, K., Stangier, M., Vemu, A., Cook, A., Wang, S., Roll-Mecak, A., Steinm...

OCA — Wed, 13 Mar 2019 10:23:27 GMT

New page: '''Unreleased structure''' The entry 6qve is ON HOLD Authors: Atherton, J.M., Luo, Y., Xiang, S., Yang, C., Jiang, K., Stangier, M., Vemu, A., Cook, A., Wang, S., Roll-Mecak, A., Steinm...

New page

'''Unreleased structure'''

The entry 6qve is ON HOLD

Authors: Atherton, J.M., Luo, Y., Xiang, S., Yang, C., Jiang, K., Stangier, M., Vemu, A., Cook, A., Wang, S., Roll-Mecak, A., Steinmetz, M.O., Akhmanova, A., Baldus, M., Moores, C.A.

Description: NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule
[[Category: Unreleased Structures]]
[[Category: Vemu, A]]
[[Category: Akhmanova, A]]
[[Category: Roll-Mecak, A]]
[[Category: Stangier, M]]
[[Category: Luo, Y]]
[[Category: Cook, A]]
[[Category: Wang, S]]
[[Category: Moores, C.A]]
[[Category: Xiang, S]]
[[Category: Baldus, M]]
[[Category: Steinmetz, M.O]]
[[Category: Yang, C]]
[[Category: Jiang, K]]
[[Category: Atherton, J.M]]