6qvs - Revision history

OCA at 12:09, 24 January 2024

OCA — Wed, 24 Jan 2024 12:09:22 GMT

←Older revision		Revision as of 12:09, 24 January 2024
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	<StructureSection load='6qvs' size='340' side='right'caption='[[6qvs]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='6qvs' size='340' side='right'caption='[[6qvs]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qvs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6QVS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QVS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ST6GAL1, SIAT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Beta-galactoside_alpha-2,6-sialyltransferase Beta-galactoside alpha-2,6-sialyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.1 2.4.99.1] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvs OCA], [https://pdbe.org/6qvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qvs RCSB], [https://www.ebi.ac.uk/pdbsum/6qvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvs ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvs OCA], [~~http~~://pdbe.org/6qvs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qvs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qvs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvs ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SIAT1_HUMAN SIAT1_HUMAN]] Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.<ref>PMID:21081508</ref>	+	[https://www.uniprot.org/uniprot/SIAT1_HUMAN SIAT1_HUMAN] Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.<ref>PMID:21081508</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6qvs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qvs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sialyltransferase 3D structures\|Sialyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-galactoside alpha-2,6-sialyltransferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Glumoff, T]]	+	[[Category: Glumoff T]]
-	[[Category: Harrus, D]]	+	[[Category: Harrus D]]
-	~~[[Category: 6-sialyltransferase 1]]~~	+
-	~~[[Category: Apo]]~~	+
-	~~[[Category: Beta-galactoside alpha-2]]~~	+
-	~~[[Category: N-linked glycosylation]]~~	+
-	~~[[Category: Sialyltransferase]]~~	+
-	~~[[Category: St6gal1]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:34, 7 October 2020

OCA — Wed, 07 Oct 2020 06:34:14 GMT

←Older revision		Revision as of 06:34, 7 October 2020
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SIAT1_HUMAN SIAT1_HUMAN]] Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.<ref>PMID:21081508</ref>		[[http://www.uniprot.org/uniprot/SIAT1_HUMAN SIAT1_HUMAN]] Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.<ref>PMID:21081508</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sialic acid residues found as terminal monosaccharides in various types of glycan chains in cell surface glycoproteins and glycolipids have been identified as important contributors of cell-cell interactions in normal vs. abnormal cellular behavior and are pivotal in diseases such as cancers. In vertebrates, sialic acids are attached to glycan chains by a conserved subset of sialyltransferases with different enzymatic and substrate specificities. ST6Gal I is a sialyltransferase using activated CMP-sialic acids as donor substrates to catalyze the formation of a alpha2,6-glycosidic bond between the sialic acid residue and the acceptor disaccharide LacNAc. Understanding sialyltransferases at the molecular and structural level shed light into their function. We present here two human ST6Gal I structures, which show for the first time the enzyme in the unliganded state and with the full donor substrate CMP-Neu5Ac bound. Comparison of these structures reveal flexibility of the catalytic loop, since in the unliganded structure Tyr354 adopts a conformation seen also as an alternate conformation in the substrate bound structure. CMP-Neu5Ac is bound with the side chain at C5 of the sugar residue directed outwards at the surface of the protein. Furthermore, the exact binding mode of the sialic acid moiety of the substrate directly involves sialylmotifs L, S and III and positions the sialylmotif VS in the immediate vicinity. We also present a model for the ternary complex of ST6Gal I with both the donor and the acceptor substrates.
		+
		+	Unliganded and CMP-Neu5Ac bound structures of human alpha-2,6-sialyltransferase ST6Gal I at high resolution.,Harrus D, Harduin-Lepers A, Glumoff T J Struct Biol. 2020 Sep 21;212(2):107628. doi: 10.1016/j.jsb.2020.107628. PMID:32971290<ref>PMID:32971290</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qvs" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:37, 17 June 2020

OCA — Wed, 17 Jun 2020 10:37:07 GMT

←Older revision		Revision as of 10:37, 17 June 2020
Line 1:		Line 1:

	==Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)==		==Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)==
-	<StructureSection load='6qvs' size='340' side='right'caption='[[6qvs]]' scene=''>	+	<StructureSection load='6qvs' size='340' side='right'caption='[[6qvs]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QVS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QVS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvs OCA], [http://pdbe.org/6qvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qvs RCSB], [http://www.ebi.ac.uk/pdbsum/6qvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST6GAL1, SIAT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactoside_alpha-2,6-sialyltransferase Beta-galactoside alpha-2,6-sialyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.1 2.4.99.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvs OCA], [http://pdbe.org/6qvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qvs RCSB], [http://www.ebi.ac.uk/pdbsum/6qvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SIAT1_HUMAN SIAT1_HUMAN]] Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.<ref>PMID:21081508</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beta-galactoside alpha-2,6-sialyltransferase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Glumoff T]]	+	[[Category: Glumoff, T]]
-	[[Category: Harrus D]]	+	[[Category: Harrus, D]]
		+	[[Category: 6-sialyltransferase 1]]
		+	[[Category: Apo]]
		+	[[Category: Beta-galactoside alpha-2]]
		+	[[Category: N-linked glycosylation]]
		+	[[Category: Sialyltransferase]]
		+	[[Category: St6gal1]]
		+	[[Category: Transferase]]

OCA at 06:24, 10 June 2020

OCA — Wed, 10 Jun 2020 06:24:35 GMT

←Older revision		Revision as of 06:24, 10 June 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6qvs is ON HOLD~~	+	==Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)==
-		+	<StructureSection load='6qvs' size='340' side='right'caption='[[6qvs]]' scene=''>
-	~~Authors: Harrus, D., Glumoff, T.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QVS FirstGlance]. <br>
-	~~Description:~~ Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvs OCA], [http://pdbe.org/6qvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qvs RCSB], [http://www.ebi.ac.uk/pdbsum/6qvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvs ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Harrus~~, D]]	+	__TOC__
-	[[Category: Glumoff, T]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Glumoff T]]
		+	[[Category: Harrus D]]

OCA: Protected "6qvs" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2019 10:25:09 GMT

Protected "6qvs" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:25, 13 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qvs is ON HOLD Authors: Harrus, D., Glumoff, T. Description: Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransfer...

OCA — Wed, 13 Mar 2019 10:25:06 GMT

New page: '''Unreleased structure''' The entry 6qvs is ON HOLD Authors: Harrus, D., Glumoff, T. Description: Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransfer...

New page

'''Unreleased structure'''

The entry 6qvs is ON HOLD

Authors: Harrus, D., Glumoff, T.

Description: Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)
[[Category: Unreleased Structures]]
[[Category: Harrus, D]]
[[Category: Glumoff, T]]