6qwk - Revision history

OCA at 12:09, 24 January 2024

OCA — Wed, 24 Jan 2024 12:09:58 GMT

←Older revision		Revision as of 12:09, 24 January 2024
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	<StructureSection load='6qwk' size='340' side='right'caption='[[6qwk]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6qwk' size='340' side='right'caption='[[6qwk]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qwk]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium~~ monocytogenes ~~hominis" nyfeldt 1932~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QWK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6QWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qwk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QWK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>prfA, CDR86_15285, D3B95_15085, DOE67_09655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1639~~ "~~Bacterium monocytogenes hominis~~" ~~Nyfeldt 1932])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6qwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qwk OCA], [~~http~~://pdbe.org/6qwk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qwk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qwk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qwk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qwk OCA], [https://pdbe.org/6qwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qwk RCSB], [https://www.ebi.ac.uk/pdbsum/6qwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qwk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PRFA_LISMO PRFA_LISMO] Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[~~Listeriolysin positive regulatory factor A~~\|~~Listeriolysin positive regulatory factor A~~]]	+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacterium monocytogenes hominis nyfeldt 1932]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brannstrom, K]]	+	[[Category: Listeria monocytogenes]]
-	[[Category: Grundstrom, C]]	+	[[Category: Brannstrom K]]
-	[[Category: Hall, M]]	+	[[Category: Grundstrom C]]
-	[[Category: Hansen, S]]	+	[[Category: Hall M]]
-	[[Category: Johansson, J]]	+	[[Category: Hansen S]]
-	[[Category: Sauer-Eriksson~~, A E]]~~	+	[[Category: Johansson J]]
-	~~[[Category: Activation]]~~	+	[[Category: Sauer-Eriksson AE]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Listeria monocytogene]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulator]]~~	+
-	~~[[Category: Virulence factor~~]]	+

OCA at 06:03, 20 May 2020

OCA — Wed, 20 May 2020 06:03:59 GMT

←Older revision		Revision as of 06:03, 20 May 2020
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	<StructureSection load='6qwk' size='340' side='right'caption='[[6qwk]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6qwk' size='340' side='right'caption='[[6qwk]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qwk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QWK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qwk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QWK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QWK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qwk OCA], [http://pdbe.org/6qwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qwk RCSB], [http://www.ebi.ac.uk/pdbsum/6qwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qwk ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">prfA, CDR86_15285, D3B95_15085, DOE67_09655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qwk OCA], [http://pdbe.org/6qwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qwk RCSB], [http://www.ebi.ac.uk/pdbsum/6qwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qwk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6qwk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qwk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Listeriolysin positive regulatory factor A\|Listeriolysin positive regulatory factor A]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium monocytogenes hominis nyfeldt 1932]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brannstrom, K]]		[[Category: Brannstrom, K]]

OCA at 09:17, 1 April 2020

OCA — Wed, 01 Apr 2020 09:17:12 GMT

←Older revision		Revision as of 09:17, 1 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6qwk is ~~ON HOLD~~	+	==The Transcriptional Regulator PrfA-L140F mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif==
		+	<StructureSection load='6qwk' size='340' side='right'caption='[[6qwk]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qwk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QWK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qwk OCA], [http://pdbe.org/6qwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qwk RCSB], [http://www.ebi.ac.uk/pdbsum/6qwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qwk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Listeria monocytogenes is a Gram-positive pathogen able to cause severe human infections. Its major virulence regulator is the transcriptional activator PrfA, a member of the Crp/Fnr family of transcriptional regulators. To establish a successful L. monocytogenes infection, the PrfA protein needs to be in an active conformation, either by binding the cognate inducer glutathione (GSH) or by possessing amino acid substitutions rendering the protein constitutively active (PrfA). By a yet unknown mechanism, PTS-sugars repress the activity of PrfA. We therefore undertook a transposon-based approach to identify the mechanism by which PTS-sugars repress PrfA activity. For this, we screened a transposon mutant bank to identify clones able to grow in presence of glucose-6-phosphate as a sole carbon source. Surprisingly, most of the isolated transposon mutants also carried amino acid substitutions in PrfA. In transposon-free strains, the PrfA amino-acid substitution mutants displayed growth, virulence factor expression, infectivity and DNA binding, agreeing with previously identified PrfA mutants. Hence, the initial growth phenotype observed in the isolated clone was due to the amino acid substitution in PrfA and unrelated to the loci inactivated by the transposon mutant. Finally, we provide structural evidence for the existence of an intermediate-activated PrfA state, which gives new insights into PrfA protein activation.IMPORTANCE The Gram-positive bacterium Listeria monocytogenes is a human pathogen mainly affecting elderly, immunocompromised people and pregnant women. It can lead to meningoencephalitis, septicaemia and abortion. The major virulence regulator in L. monocytogenes is the PrfA protein, a transcriptional activator. Using a growth-based selection strategy, we identified mutations in the PrfA protein leading to constitutively active virulence factor expression. We provide structural evidence for the existence of an intermediate-activated PrfA state, which gives new insights into PrfA protein activation.

-	~~Authors~~:	+	A novel growth based selection strategy identifies new constitutively active variants of the major virulence regulator PrfA in Listeria monocytogenes.,Hansen S, Hall M, Grundstrom C, Brannstrom K, Sauer-Eriksson AE, Johansson J J Bacteriol. 2020 Mar 16. pii: JB.00115-20. doi: 10.1128/JB.00115-20. PMID:32179627<ref>PMID:32179627</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6qwk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Brannstrom, K]]
		+	[[Category: Grundstrom, C]]
		+	[[Category: Hall, M]]
		+	[[Category: Hansen, S]]
		+	[[Category: Johansson, J]]
		+	[[Category: Sauer-Eriksson, A E]]
		+	[[Category: Activation]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Listeria monocytogene]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription regulator]]
		+	[[Category: Virulence factor]]

OCA: Protected "6qwk" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2019 10:28:27 GMT

Protected "6qwk" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:28, 13 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qwk is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Mar 2019 10:28:25 GMT

New page: '''Unreleased structure''' The entry 6qwk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qwk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]