6qx4 - Revision history

OCA at 12:10, 24 January 2024

OCA — Wed, 24 Jan 2024 12:10:20 GMT

←Older revision		Revision as of 12:10, 24 January 2024
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	<StructureSection load='6qx4' size='340' side='right'caption='[[6qx4]], [[Resolution\|resolution]] 3.27Å' scene=''>		<StructureSection load='6qx4' size='340' side='right'caption='[[6qx4]], [[Resolution\|resolution]] 3.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qx4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var.~~ anthracis~~" (cohn 1872) smith et al. 1946~~] and [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QX4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qx4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QX4 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~sap~~, ~~BA_0885, GBAA_0885, BAS0841 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.27Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qx4 OCA], [~~http~~://pdbe.org/6qx4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qx4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qx4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qx4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qx4 OCA], [https://pdbe.org/6qx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qx4 RCSB], [https://www.ebi.ac.uk/pdbsum/6qx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qx4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SLAP1_BACAN SLAP1_BACAN]] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria.	+	[https://www.uniprot.org/uniprot/SLAP1_BACAN SLAP1_BACAN] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Bacillus anthracis]]
		+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fioravanti, A]]	+	[[Category: Fioravanti A]]
-	[[Category: Remaut, H]]	+	[[Category: Remaut H]]
-	~~[[Category: Bacterial cell surface]]~~	+
-	~~[[Category: Exoskeleton]]~~	+
-	~~[[Category: S-layer]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 08:40, 6 November 2019

OCA — Wed, 06 Nov 2019 08:40:19 GMT

←Older revision		Revision as of 08:40, 6 November 2019
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	<StructureSection load='6qx4' size='340' side='right'caption='[[6qx4]], [[Resolution\|resolution]] 3.27Å' scene=''>		<StructureSection load='6qx4' size='340' side='right'caption='[[6qx4]], [[Resolution\|resolution]] 3.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qx4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qx4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QX4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qx4 OCA], [http://pdbe.org/6qx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qx4 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sap, BA_0885, GBAA_0885, BAS0841 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qx4 OCA], [http://pdbe.org/6qx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qx4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fioravanti, A]]		[[Category: Fioravanti, A]]

OCA at 06:30, 31 July 2019

OCA — Wed, 31 Jul 2019 06:30:37 GMT

←Older revision		Revision as of 06:30, 31 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6qx4 is ~~ON HOLD~~	+	==Structure of the Bacillus anthracis Sap S-layer assembly domain==
		+	<StructureSection load='6qx4' size='340' side='right'caption='[[6qx4]], [[Resolution\|resolution]] 3.27Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qx4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QX4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qx4 OCA], [http://pdbe.org/6qx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qx4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SLAP1_BACAN SLAP1_BACAN]] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anthrax is an ancient and deadly disease caused by the spore-forming bacterial pathogen Bacillus anthracis. At present, anthrax mostly affects wildlife and livestock, although it remains a concern for human public health-primarily for people who handle contaminated animal products and as a bioterrorism threat due to the high resilience of spores, a high fatality rate of cases and the lack of a civilian vaccination programme(1,2). The cell surface of B. anthracis is covered by a protective paracrystalline monolayer-known as surface layer or S-layer-that is composed of the S-layer proteins Sap or EA1. Here, we generate nanobodies to inhibit the self-assembly of Sap, determine the structure of the Sap S-layer assembly domain (Sap(AD)) and show that the disintegration of the S-layer attenuates the growth of B. anthracis and the pathology of anthrax in vivo. Sap(AD) comprises six beta-sandwich domains that fold and support the formation of S-layers independently of calcium. Sap-inhibitory nanobodies prevented the assembly of Sap and depolymerized existing Sap S-layers in vitro. In vivo, nanobody-mediated disruption of the Sap S-layer resulted in severe morphological defects and attenuated bacterial growth. Subcutaneous delivery of Sap inhibitory nanobodies cleared B. anthracis infection and prevented lethality in a mouse model of anthrax disease. These findings highlight disruption of S-layer integrity as a mechanism that has therapeutic potential in S-layer-carrying pathogens.

-	~~Authors: Remaut, H~~., Fioravanti, A.	+	Structure of S-layer protein Sap reveals a mechanism for therapeutic intervention in anthrax.,Fioravanti A, Van Hauwermeiren F, Van der Verren SE, Jonckheere W, Goncalves A, Pardon E, Steyaert J, De Greve H, Lamkanfi M, Remaut H Nat Microbiol. 2019 Jul 15. pii: 10.1038/s41564-019-0499-1. doi:, 10.1038/s41564-019-0499-1. PMID:31308522<ref>PMID:31308522</ref>

-	~~Description: Structure~~ of the ~~Bacillus anthracis Sap~~ S-~~layer assembly domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6qx4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Fioravanti, A]]		[[Category: Fioravanti, A]]
	[[Category: Remaut, H]]		[[Category: Remaut, H]]
		+	[[Category: Bacterial cell surface]]
		+	[[Category: Exoskeleton]]
		+	[[Category: S-layer]]
		+	[[Category: Structural protein]]

OCA: Protected "6qx4" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2019 10:29:35 GMT

Protected "6qx4" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:29, 13 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qx4 is ON HOLD Authors: Remaut, H., Fioravanti, A. Description: Structure of the Bacillus anthracis Sap S-layer assembly domain [[Category: Unrele...

OCA — Wed, 13 Mar 2019 10:29:34 GMT

New page: '''Unreleased structure''' The entry 6qx4 is ON HOLD Authors: Remaut, H., Fioravanti, A. Description: Structure of the Bacillus anthracis Sap S-layer assembly domain [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 6qx4 is ON HOLD

Authors: Remaut, H., Fioravanti, A.

Description: Structure of the Bacillus anthracis Sap S-layer assembly domain
[[Category: Unreleased Structures]]
[[Category: Fioravanti, A]]
[[Category: Remaut, H]]