6qxg - Revision history

OCA at 12:10, 24 January 2024

OCA — Wed, 24 Jan 2024 12:10:25 GMT

←Older revision		Revision as of 12:10, 24 January 2024
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	<StructureSection load='6qxg' size='340' side='right'caption='[[6qxg]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='6qxg' size='340' side='right'caption='[[6qxg]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qxg]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QXG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qxg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QXG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~>, ~~<scene name='pdbligand=UFP:5-FLUORO-~~2~~-DEOXYURIDINE-5-MONOPHOSPHATE'>UFP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>TYMS, TS, OK/SW-cl.29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UFP:5-FLUORO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>UFP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~2~~.1.1.45 2.1.1.45]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qxg OCA], [https://pdbe.org/6qxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qxg RCSB], [https://www.ebi.ac.uk/pdbsum/6qxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qxg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qxg OCA], [~~http~~://pdbe.org/6qxg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qxg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qxg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qxg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TYSY_HUMAN TYSY_HUMAN]] Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.<ref>PMID:21876188</ref>	+	[https://www.uniprot.org/uniprot/TYSY_HUMAN TYSY_HUMAN] Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.<ref>PMID:21876188</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6qxg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qxg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Thymidylate synthase]]~~	+	[[Category: Mangani M]]
-	[[Category: Mangani, M]]	+	[[Category: Pozzi C]]
-	[[Category: Pozzi, C]]	+
-	~~[[Category: Fdump]]~~	+
-	~~[[Category: Folate pathway]]~~	+
-	~~[[Category: Human thymidylate synthase]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:38, 10 July 2019

OCA — Wed, 10 Jul 2019 06:38:52 GMT

←Older revision		Revision as of 06:38, 10 July 2019
Line 3:		Line 3:
	<StructureSection load='6qxg' size='340' side='right'caption='[[6qxg]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='6qxg' size='340' side='right'caption='[[6qxg]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qxg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qxg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QXG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UFP:5-FLUORO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>UFP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UFP:5-FLUORO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>UFP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TYMS, TS, OK/SW-cl.29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qxg OCA], [http://pdbe.org/6qxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qxg RCSB], [http://www.ebi.ac.uk/pdbsum/6qxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qxg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qxg OCA], [http://pdbe.org/6qxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qxg RCSB], [http://www.ebi.ac.uk/pdbsum/6qxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qxg ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Thymidylate synthase]]		[[Category: Thymidylate synthase]]

OCA at 07:31, 10 April 2019

OCA — Wed, 10 Apr 2019 07:31:13 GMT

←Older revision		Revision as of 07:31, 10 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qxg is ~~ON HOLD~~	+	==Crystal structure of His-tag human thymidylate synthase (HT-hTS) in complex with FdUMP==
		+	<StructureSection load='6qxg' size='340' side='right'caption='[[6qxg]], [[Resolution\|resolution]] 2.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qxg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QXG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UFP:5-FLUORO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>UFP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qxg OCA], [http://pdbe.org/6qxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qxg RCSB], [http://www.ebi.ac.uk/pdbsum/6qxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qxg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TYSY_HUMAN TYSY_HUMAN]] Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.<ref>PMID:21876188</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thymidylate synthase (TS) is an enzyme of paramount importance as it provides the only de novo source of deoxy-thymidine monophosphate (dTMP). dTMP, essential for DNA synthesis, is produced by the TS-catalyzed reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) using N(5),N(10)-methylenetetrahydrofolate (mTHF) as a cofactor. TS is ubiquitous and a validated drug target. TS enzymes from different organisms differ in sequence and structure, but are all obligate homodimers. The structural and mechanistic differences between the human and bacterial enzymes are exploitable to obtain selective inhibitors of bacterial TSs that can enrich the currently available therapeutic tools against bacterial infections. Enterococcus faecalis is a pathogen fully dependent on TS for dTMP synthesis. In this study, we present four new crystal structures of Enterococcus faecalis and human TSs in complex with either the substrate dUMP or the inhibitor FdUMP. The results provide new clues about the half-site reactivity of Enterococcus faecalis TS and the mechanisms underlying the conformational changes occurring in the two enzymes. We also identify relevant differences in cofactor and inhibitor binding between Enterococcus faecalis and human TS that can guide the design of selective inhibitors against bacterial TSs.

-	~~Authors: Pozzi~~, C., Mangani, M.	+	Structural Comparison of Enterococcus faecalis and Human Thymidylate Synthase Complexes with the Substrate dUMP and Its Analogue FdUMP Provides Hints about Enzyme Conformational Variabilities.,Pozzi C, Ferrari S, Luciani R, Tassone G, Costi MP, Mangani S Molecules. 2019 Mar 31;24(7). pii: molecules24071257. doi:, 10.3390/molecules24071257. PMID:30935102<ref>PMID:30935102</ref>

-	~~Description: Crystal structure~~ of ~~His~~-~~tag human thymidylate synthase (HT~~-~~hTS) in complex with FdUMP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6qxg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thymidylate synthase]]
	[[Category: Mangani, M]]		[[Category: Mangani, M]]
	[[Category: Pozzi, C]]		[[Category: Pozzi, C]]
		+	[[Category: Fdump]]
		+	[[Category: Folate pathway]]
		+	[[Category: Human thymidylate synthase]]
		+	[[Category: Inhibitor]]
		+	[[Category: Transferase]]

OCA: Protected "6qxg" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2019 10:30:45 GMT

Protected "6qxg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:30, 13 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qxg is ON HOLD Authors: Pozzi, C., Mangani, M. Description: Crystal structure of His-tag human thymidylate synthase (HT-hTS) in complex with FdUMP...

OCA — Wed, 13 Mar 2019 10:30:43 GMT

New page: '''Unreleased structure''' The entry 6qxg is ON HOLD Authors: Pozzi, C., Mangani, M. Description: Crystal structure of His-tag human thymidylate synthase (HT-hTS) in complex with FdUMP...

New page

'''Unreleased structure'''

The entry 6qxg is ON HOLD

Authors: Pozzi, C., Mangani, M.

Description: Crystal structure of His-tag human thymidylate synthase (HT-hTS) in complex with FdUMP
[[Category: Unreleased Structures]]
[[Category: Mangani, M]]
[[Category: Pozzi, C]]