6qyh - Revision history

OCA at 12:11, 24 January 2024

OCA — Wed, 24 Jan 2024 12:11:08 GMT

←Older revision		Revision as of 12:11, 24 January 2024
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	<StructureSection load='6qyh' size='340' side='right'caption='[[6qyh]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='6qyh' size='340' side='right'caption='[[6qyh]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qyh]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QYH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QYH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qyh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QYH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hpaB, CR538_21835, DS732_04180, NCTC9045_04991, NCTC9117_05338 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylacetate_3-monooxygenase 4-hydroxyphenylacetate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.9 1.14.14.9] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qyh OCA], [https://pdbe.org/6qyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qyh RCSB], [https://www.ebi.ac.uk/pdbsum/6qyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qyh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qyh OCA], [~~http~~://pdbe.org/6qyh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qyh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qyh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qyh ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HPAB_ECOLX HPAB_ECOLX] Utilizes FADH(2) supplied by HpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives.<ref>PMID:10653707</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6qyh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qyh" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: 4-hydroxyphenylacetate 3-monooxygenase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Deng, Y]]	+	[[Category: Deng Y]]
-	[[Category: Fontecave, M]]	+	[[Category: Fontecave M]]
-	[[Category: Lombard, M]]	+	[[Category: Lombard M]]
-	[[Category: Pecqueur, L]]	+	[[Category: Pecqueur L]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase monooxygenase~~]]	+

OCA at 16:17, 29 January 2020

OCA — Wed, 29 Jan 2020 16:17:24 GMT

←Older revision		Revision as of 16:17, 29 January 2020
Line 3:		Line 3:
	<StructureSection load='6qyh' size='340' side='right'caption='[[6qyh]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='6qyh' size='340' side='right'caption='[[6qyh]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qyh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QYH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qyh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QYH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hpaB, CR538_21835, DS732_04180, NCTC9045_04991, NCTC9117_05338 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylacetate_3-monooxygenase 4-hydroxyphenylacetate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.9 1.14.14.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylacetate_3-monooxygenase 4-hydroxyphenylacetate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.9 1.14.14.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qyh OCA], [http://pdbe.org/6qyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qyh RCSB], [http://www.ebi.ac.uk/pdbsum/6qyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qyh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qyh OCA], [http://pdbe.org/6qyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qyh RCSB], [http://www.ebi.ac.uk/pdbsum/6qyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qyh ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]		[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 05:47, 12 June 2019

OCA — Wed, 12 Jun 2019 05:47:26 GMT

←Older revision		Revision as of 05:47, 12 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qyh is ~~ON HOLD until Paper~~ Publication	+	==Structure of Apo HPAB from E.coli==
		+	<StructureSection load='6qyh' size='340' side='right'caption='[[6qyh]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qyh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QYH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylacetate_3-monooxygenase 4-hydroxyphenylacetate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.9 1.14.14.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qyh OCA], [http://pdbe.org/6qyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qyh RCSB], [http://www.ebi.ac.uk/pdbsum/6qyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qyh ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hydroxylation of phenols into polyphenols, valuable chemical and pharmaceutical products, is a challenging reaction. The search for green synthetic processes has led to considering microorganisms as well as pure hydroxylases as catalysts for phenol hydroxylation. Here we report the structural and functional characterization of the FAD-dependent 4-hydroxyphenylacetate 3-monooxygenase from Escherichia coli, named HpaB. We show that this enzyme enjoys a relatively broad substrate specificity, allowing conversion of a number of non-natural phenolic compounds such as tyrosol, hydroxymandelic acid, coumaric acid, hydroxybenzoic acid and its methyl ester and phenol into the corresponding catechols. The reaction can be carried out using a simple chemical assay based on formate as the electron donor and the organometallic complex [Cp*Rh(bpy)(H2O)]2+ as the catalyst for FAD reduction. The availability of a crystal structure of HpaB in complex with FAD 1.8 A resolution opens the possibility of rational tuning of the substrate specificity and the activity of this interesting class of phenol hydroxylases.

-	~~Authors~~:	+	Structure and functional characterization of 4-hydroxyphenylacetate 3-hydroxylase from Escherichia coli.,Deng Y, Faivre B, Back O, Lombard M, Pecqueur L, Fontecave M Chembiochem. 2019 Jun 2. doi: 10.1002/cbic.201900277. PMID:31155821<ref>PMID:31155821</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6qyh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
		+	[[Category: Large Structures]]
		+	[[Category: Deng, Y]]
		+	[[Category: Fontecave, M]]
		+	[[Category: Lombard, M]]
		+	[[Category: Pecqueur, L]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxidoreductase monooxygenase]]

OCA: Protected "6qyh" [edit=sysop:move=sysop]

OCA — Wed, 20 Mar 2019 06:08:53 GMT

Protected "6qyh" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:08, 20 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qyh is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Mar 2019 06:08:51 GMT

New page: '''Unreleased structure''' The entry 6qyh is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qyh is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]