6qz4 - Revision history

OCA at 12:11, 24 January 2024

OCA — Wed, 24 Jan 2024 12:11:29 GMT

←Older revision		Revision as of 12:11, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6qz4' size='340' side='right'caption='[[6qz4]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6qz4' size='340' side='right'caption='[[6qz4]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qz4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Idesa Idesa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QZ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6QZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qz4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QZ4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ISF6_0224 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1547922 IDESA])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [https://pdbe.org/6qz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [https://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [~~http~~://pdbe.org/6qz4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MHETH_IDESA MHETH_IDESA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Idesa~~]]	+	[[Category: Ideonella sakaiensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, M D~~]]	+	[[Category: Allen MD]]
-	[[Category: Beckham~~, G T~~]]	+	[[Category: Beckham GT]]
-	[[Category: Johnson~~, C W~~]]	+	[[Category: Johnson CW]]
-	[[Category: Knott~~, B C~~]]	+	[[Category: Knott BC]]
-	[[Category: McGeehan~~, J E]]~~	+	[[Category: McGeehan JE]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mhetase]]~~	+
-	~~[[Category: Pet degradation]]~~	+
-	~~[[Category: Plastic-binding protein]]~~	+
-	~~[[Category: Structural genomic~~]]	+

OCA at 06:10, 14 October 2020

OCA — Wed, 14 Oct 2020 06:10:56 GMT

←Older revision		Revision as of 06:10, 14 October 2020
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [http://pdbe.org/6qz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [http://pdbe.org/6qz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plastics pollution represents a global environmental crisis. In response, microbes are evolving the capacity to utilize synthetic polymers as carbon and energy sources. Recently, Ideonella sakaiensis was reported to secrete a two-enzyme system to deconstruct polyethylene terephthalate (PET) to its constituent monomers. Specifically, the I. sakaiensis PETase depolymerizes PET, liberating soluble products, including mono(2-hydroxyethyl) terephthalate (MHET), which is cleaved to terephthalic acid and ethylene glycol by MHETase. Here, we report a 1.6 A resolution MHETase structure, illustrating that the MHETase core domain is similar to PETase, capped by a lid domain. Simulations of the catalytic itinerary predict that MHETase follows the canonical two-step serine hydrolase mechanism. Bioinformatics analysis suggests that MHETase evolved from ferulic acid esterases, and two homologous enzymes are shown to exhibit MHET turnover. Analysis of the two homologous enzymes and the MHETase S131G mutant demonstrates the importance of this residue for accommodation of MHET in the active site. We also demonstrate that the MHETase lid is crucial for hydrolysis of MHET and, furthermore, that MHETase does not turnover mono(2-hydroxyethyl)-furanoate or mono(2-hydroxyethyl)-isophthalate. A highly synergistic relationship between PETase and MHETase was observed for the conversion of amorphous PET film to monomers across all nonzero MHETase concentrations tested. Finally, we compare the performance of MHETase:PETase chimeric proteins of varying linker lengths, which all exhibit improved PET and MHET turnover relative to the free enzymes. Together, these results offer insights into the two-enzyme PET depolymerization system and will inform future efforts in the biological deconstruction and upcycling of mixed plastics.
		+
		+	Characterization and engineering of a two-enzyme system for plastics depolymerization.,Knott BC, Erickson E, Allen MD, Gado JE, Graham R, Kearns FL, Pardo I, Topuzlu E, Anderson JJ, Austin HP, Dominick G, Johnson CW, Rorrer NA, Szostkiewicz CJ, Copie V, Payne CM, Woodcock HL, Donohoe BS, Beckham GT, McGeehan JE Proc Natl Acad Sci U S A. 2020 Sep 28. pii: 2006753117. doi:, 10.1073/pnas.2006753117. PMID:32989159<ref>PMID:32989159</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qz4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:35, 7 October 2020

OCA — Wed, 07 Oct 2020 06:35:04 GMT

←Older revision		Revision as of 06:35, 7 October 2020
Line 1:		Line 1:

	==Structure of MHETase from Ideonella sakaiensis==		==Structure of MHETase from Ideonella sakaiensis==
-	<StructureSection load='6qz4' size='340' side='right'caption='[[6qz4]]' scene=''>	+	<StructureSection load='6qz4' size='340' side='right'caption='[[6qz4]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qz4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Idesa Idesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QZ4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [http://pdbe.org/6qz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISF6_0224 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1547922 IDESA])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [http://pdbe.org/6qz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Idesa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen MD]]	+	[[Category: Allen, M D]]
-	[[Category: Beckham GT]]	+	[[Category: Beckham, G T]]
-	[[Category: Johnson CW]]	+	[[Category: Johnson, C W]]
-	[[Category: Knott BC]]	+	[[Category: Knott, B C]]
-	[[Category: McGeehan JE]]	+	[[Category: McGeehan, J E]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mhetase]]
		+	[[Category: Pet degradation]]
		+	[[Category: Plastic-binding protein]]
		+	[[Category: Structural genomic]]

OCA at 07:04, 30 September 2020

OCA — Wed, 30 Sep 2020 07:04:59 GMT

←Older revision		Revision as of 07:04, 30 September 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qz4 ~~is ON HOLD until Paper Publication~~	+	==Structure of MHETase from Ideonella sakaiensis==
-		+	<StructureSection load='6qz4' size='340' side='right'caption='[[6qz4]]' scene=''>
-	~~Authors~~: ~~Allen, M~~.D., ~~Johnson, C~~.W., ~~Knott~~, B.C., ~~Beckham, G~~.T., ~~McGeehan, J~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QZ4 FirstGlance]. <br>
-	~~Description: Structure of MHETase from Ideonella sakaiensis~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qz4 OCA], [http://pdbe.org/6qz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6qz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qz4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Mcgeehan, J.E~~]]	+	__TOC__
-	[[Category: ~~Allen, M.D~~]]	+	</StructureSection>
-	[[Category: ~~Knott, B.C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Johnson, C.W~~]]	+	[[Category: Allen MD]]
-	[[Category: ~~Beckham, G.T~~]]	+	[[Category: Beckham GT]]
		+	[[Category: Johnson CW]]
		+	[[Category: Knott BC]]
		+	[[Category: McGeehan JE]]

OCA at 13:50, 22 January 2020

OCA — Wed, 22 Jan 2020 13:50:42 GMT

←Older revision		Revision as of 13:50, 22 January 2020
Line 5:		Line 5:
	Authors: Allen, M.D., Johnson, C.W., Knott, B.C., Beckham, G.T., McGeehan, J.E.		Authors: Allen, M.D., Johnson, C.W., Knott, B.C., Beckham, G.T., McGeehan, J.E.

-	Description: Structure of ~~from~~ MHETase from Ideonella sakaiensis	+	Description: Structure of MHETase from Ideonella sakaiensis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Mcgeehan, J.E]]		[[Category: Mcgeehan, J.E]]

OCA: Protected "6qz4" [edit=sysop:move=sysop]

OCA — Wed, 20 Mar 2019 06:10:58 GMT

Protected "6qz4" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:10, 20 March 2019

OCA: New page: '''Unreleased structure''' The entry 6qz4 is ON HOLD until Paper Publication Authors: Allen, M.D., Johnson, C.W., Knott, B.C., Beckham, G.T., McGeehan, J.E. Description: Structure of f...

OCA — Wed, 20 Mar 2019 06:10:56 GMT

New page: '''Unreleased structure''' The entry 6qz4 is ON HOLD until Paper Publication Authors: Allen, M.D., Johnson, C.W., Knott, B.C., Beckham, G.T., McGeehan, J.E. Description: Structure of f...

New page

'''Unreleased structure'''

The entry 6qz4 is ON HOLD until Paper Publication

Authors: Allen, M.D., Johnson, C.W., Knott, B.C., Beckham, G.T., McGeehan, J.E.

Description: Structure of from MHETase from Ideonella sakaiensis
[[Category: Unreleased Structures]]
[[Category: Mcgeehan, J.E]]
[[Category: Allen, M.D]]
[[Category: Knott, B.C]]
[[Category: Johnson, C.W]]
[[Category: Beckham, G.T]]