6r6o - Revision history

OCA at 12:16, 24 January 2024

OCA — Wed, 24 Jan 2024 12:16:12 GMT

←Older revision		Revision as of 12:16, 24 January 2024
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	<StructureSection load='6r6o' size='340' side='right'caption='[[6r6o]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6r6o' size='340' side='right'caption='[[6r6o]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r6o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"candidatus_kuenenia_stuttgartiensis"_schmid_et_al._2000 "candidatus kuenenia~~ stuttgartiensis~~" schmid et al. 2000~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6R6O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r6o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Kuenenia_stuttgartiensis Candidatus Kuenenia stuttgartiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R6O FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HEC:HEME+C'>HEC</scene~~>, ~~<scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>KSMBR1_3082, kusta0087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~174633~~ "~~Candidatus Kuenenia stuttgartiensis~~" ~~Schmid et al. 2000]), KSMBR1_3083, kusta0088 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=174633 "Candidatus Kuenenia stuttgartiensis" Schmid et al. 2000])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6r6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6o OCA], [~~http~~://pdbe.org/6r6o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6r6o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6r6o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6o OCA], [https://pdbe.org/6r6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r6o RCSB], [https://www.ebi.ac.uk/pdbsum/6r6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q1Q7P4_KUEST Q1Q7P4_KUEST]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Candidatus ~~kuenenia~~ stuttgartiensis ~~schmid et al. 2000~~]]	+	[[Category: Candidatus Kuenenia stuttgartiensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akram, M]]	+	[[Category: Akram M]]
-	[[Category: Barends, T]]	+	[[Category: Barends T]]
-	~~[[Category: Cystein ligation]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Heme-c~~]]	+

OCA at 08:08, 23 October 2019

OCA — Wed, 23 Oct 2019 08:08:03 GMT

←Older revision		Revision as of 08:08, 23 October 2019
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	<StructureSection load='6r6o' size='340' side='right'caption='[[6r6o]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6r6o' size='340' side='right'caption='[[6r6o]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r6o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R6O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r6o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"candidatus_kuenenia_stuttgartiensis"_schmid_et_al._2000 "candidatus kuenenia stuttgartiensis" schmid et al. 2000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R6O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSMBR1_3082, kusta0087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=174633 "Candidatus Kuenenia stuttgartiensis" Schmid et al. 2000]), KSMBR1_3083, kusta0088 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=174633 "Candidatus Kuenenia stuttgartiensis" Schmid et al. 2000])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6o OCA], [http://pdbe.org/6r6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r6o RCSB], [http://www.ebi.ac.uk/pdbsum/6r6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6o OCA], [http://pdbe.org/6r6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r6o RCSB], [http://www.ebi.ac.uk/pdbsum/6r6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6o ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candidatus kuenenia stuttgartiensis schmid et al. 2000]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Akram, M]]		[[Category: Akram, M]]

OCA at 10:39, 2 October 2019

OCA — Wed, 02 Oct 2019 10:39:37 GMT

←Older revision		Revision as of 10:39, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6r6o is ~~ON HOLD until Paper~~ Publication	+	==Recombinantly produced Kusta0087/Kusta0088 Complex, C32G/wt mutant==
		+	<StructureSection load='6r6o' size='340' side='right'caption='[[6r6o]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6r6o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R6O FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6o OCA], [http://pdbe.org/6r6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r6o RCSB], [http://www.ebi.ac.uk/pdbsum/6r6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6o ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anaerobic ammonium oxidation (anammox) is a microbial process responsible for significant nitrogen loss from the oceans and other ecosystems. The redox reactions at the heart of anammox are catalyzed by large multiheme enzyme complexes that rely on small cytochrome c proteins for electron shuttling. Among the most highly abundant of these cytochromes is a unique heterodimeric complex composed of class I and class II c-type cytochrome called NaxLS, which has distinctive biochemical and spectroscopic properties. Here, we present the 1.7 A resolution crystal structure of this complex from the anammox organism Kuenenia stuttgartiensis (KsNaxLS). The structure reveals that the heme irons in each subunit exhibit a rare His/Cys ligation, which, as we show by substitution, causes the observed unusual spectral properties. Unlike its individual subunits, the KsNaxLS complex binds nitric oxide (NO) only at the distal heme side, forming 6cNO adducts. This is likely due to steric immobilization of the proximal heme binding motifs upon complex formation, a finding that may be of functional relevance, since NO is an intermediate in the central anammox metabolism. Pulldown experiments with K. stuttgartiensis cell-free extract showed that the KsNaxLS complex binds specifically to one of the central anammox enzyme complexes, hydrazine synthase, which uses NO as one of its substrates. It is therefore possible that the KsNaxLS complex plays a role in binding the volatile NO to retain it in the cell for transfer to hydrazine synthase. Alternatively, we propose that KsNaxLS may shuttle electrons to this enzyme complex.

-	~~Authors: Akram~~, M., ~~Barends~~, T.	+	A nitric oxide-binding heterodimeric cytochrome c complex from the anammox bacterium Kuenenia stuttgartiensis binds to hydrazine synthase.,Akram M, Reimann J, Dietl A, Menzel A, Versantvoort W, Kartal B, Jetten MSM, Barends TRM J Biol Chem. 2019 Sep 22. pii: RA119.008788. doi: 10.1074/jbc.RA119.008788. PMID:31548310<ref>PMID:31548310</ref>

-	~~Description: Recombinantly produced Kusta0087~~/~~Kusta0088 Complex~~, ~~C32G~~/~~wt mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6r6o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Akram, M]]		[[Category: Akram, M]]
	[[Category: Barends, T]]		[[Category: Barends, T]]
		+	[[Category: Cystein ligation]]
		+	[[Category: Electron transport]]
		+	[[Category: Heme-c]]

OCA at 05:39, 10 April 2019

OCA — Wed, 10 Apr 2019 05:39:03 GMT

←Older revision		Revision as of 05:39, 10 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6r6o is ON HOLD	+	The entry 6r6o is ON HOLD until Paper Publication

	Authors: Akram, M., Barends, T.		Authors: Akram, M., Barends, T.

OCA: Protected "6r6o" [edit=sysop:move=sysop]

OCA — Wed, 03 Apr 2019 05:03:42 GMT

Protected "6r6o" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 3 April 2019

OCA: New page: '''Unreleased structure''' The entry 6r6o is ON HOLD Authors: Akram, M., Barends, T. Description: Recombinantly produced Kusta0087/Kusta0088 Complex, C32G/wt mutant [[Category: Unrelea...

OCA — Wed, 03 Apr 2019 05:03:40 GMT

New page: '''Unreleased structure''' The entry 6r6o is ON HOLD Authors: Akram, M., Barends, T. Description: Recombinantly produced Kusta0087/Kusta0088 Complex, C32G/wt mutant [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 6r6o is ON HOLD

Authors: Akram, M., Barends, T.

Description: Recombinantly produced Kusta0087/Kusta0088 Complex, C32G/wt mutant
[[Category: Unreleased Structures]]
[[Category: Akram, M]]
[[Category: Barends, T]]