6rgp - Revision history

OCA at 12:20, 24 January 2024

OCA — Wed, 24 Jan 2024 12:20:05 GMT

←Older revision		Revision as of 12:20, 24 January 2024
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	<StructureSection load='6rgp' size='340' side='right'caption='[[6rgp]], [[Resolution\|resolution]] 0.97Å' scene=''>		<StructureSection load='6rgp' size='340' side='right'caption='[[6rgp]], [[Resolution\|resolution]] 0.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rgp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Steccherinum_murashkinskyi Steccherinum murashkinskyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RGP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RGP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rgp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Steccherinum_murashkinskyi Steccherinum murashkinskyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RGP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.97Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rgp OCA], [~~http~~://pdbe.org/6rgp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rgp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rgp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rgp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rgp OCA], [https://pdbe.org/6rgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rgp RCSB], [https://www.ebi.ac.uk/pdbsum/6rgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rgp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/I1VE66_9APHY I1VE66_9APHY]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Laccases are enzymes that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of molecular oxygen to water. Here, a subatomic resolution X-ray crystallographic study of the mechanism of inhibition of the laccase from the basidiomycete fungus Steccherinum murashkinskyi by chloride and fluoride ions is presented. Three series of X-ray diffraction data sets were collected with increasing doses of absorbed X-ray radiation from a native S. murashkinskyi laccase crystal and from crystals of complexes of the laccase with chloride and fluoride ions. The data for the native laccase crystal confirmed the previously deduced enzymatic mechanism of molecular oxygen reduction. The structures of the complexes allowed the localization of chloride and fluoride ions in the channel near the T2 copper ion. These ions replace the oxygen ligand of the T2 copper ion in this channel and can play the role of this ligand in the enzymatic reaction. As follows from analysis of the structures from the increasing dose series, the inhibition of laccases by chloride and fluoride anions can be explained by the fact that the binding of these negatively charged ions at the position of the oxygen ligand of the T2 copper ion impedes the reduction of the T2 copper ion.
		+
		+	The subatomic resolution study of laccase inhibition by chloride and fluoride anions using single-crystal serial crystallography: insights into the enzymatic reaction mechanism.,Polyakov KM, Gavryushov S, Fedorova TV, Glazunova OA, Popov AN Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):804-816. doi: , 10.1107/S2059798319010684. Epub 2019 Aug 22. PMID:31478903<ref>PMID:31478903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6rgp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Laccase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Steccherinum murashkinskyi]]		[[Category: Steccherinum murashkinskyi]]
-	[[Category: Fedorova~~, T V~~]]	+	[[Category: Fedorova TV]]
-	[[Category: Gavryushov, S]]	+	[[Category: Gavryushov S]]
-	[[Category: Glazunova~~, O A~~]]	+	[[Category: Glazunova OA]]
-	[[Category: Polyakov~~, K M~~]]	+	[[Category: Polyakov KM]]
-	[[Category: Popov~~, A N]]~~	+	[[Category: Popov AN]]
-	~~[[Category: Complex with molecular oxygen]]~~	+
-	~~[[Category: Multi copper oxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:58, 1 May 2019

OCA — Wed, 01 May 2019 08:58:52 GMT

←Older revision		Revision as of 08:58, 1 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6rgp is ON HOLD~~	+	==Single crystal serial study of the X-ray induced enzymatic reduction of molecular oxygen to water for laccase from Steccherinum murashkinskyi at sub-atomic resolution. Second structure of the series with 165 KGy dose.==
-		+	<StructureSection load='6rgp' size='340' side='right'caption='[[6rgp]], [[Resolution\|resolution]] 0.97Å' scene=''>
-	~~Authors: Polyakov, K.M., Gavryushov, S., Fedorova, T.V., Glazunova, O.A., Popov, A.N.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6rgp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Steccherinum_murashkinskyi Steccherinum murashkinskyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RGP FirstGlance]. <br>
-	~~Description:~~ Single crystal serial study of the X-ray induced enzymatic reduction of molecular oxygen to water for laccase from Steccherinum murashkinskyi at sub-atomic resolution. Second structure of the series with 165 KGy dose.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	[[~~Category~~: ~~Fedorova~~, T.V]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rgp OCA], [http://pdbe.org/6rgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rgp RCSB], [http://www.ebi.ac.uk/pdbsum/6rgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rgp ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Popov~~, A.N]]	+	</table>
-	[[Category: ~~Glazunova~~, ~~O.A~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Laccase]]
		+	[[Category: Large Structures]]
		+	[[Category: Steccherinum murashkinskyi]]
		+	[[Category: Fedorova, T V]]
	[[Category: Gavryushov, S]]		[[Category: Gavryushov, S]]
-	[[Category: Polyakov, K.M]]	+	[[Category: Glazunova, O A]]
		+	[[Category: Polyakov, K M]]
		+	[[Category: Popov, A N]]
		+	[[Category: Complex with molecular oxygen]]
		+	[[Category: Multi copper oxidase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6rgp" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2019 05:18:11 GMT

Protected "6rgp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 24 April 2019

OCA: New page: '''Unreleased structure''' The entry 6rgp is ON HOLD Authors: Polyakov, K.M., Gavryushov, S., Fedorova, T.V., Glazunova, O.A., Popov, A.N. Description: Single crystal serial study of t...

OCA — Wed, 24 Apr 2019 05:18:09 GMT

New page: '''Unreleased structure''' The entry 6rgp is ON HOLD Authors: Polyakov, K.M., Gavryushov, S., Fedorova, T.V., Glazunova, O.A., Popov, A.N. Description: Single crystal serial study of t...

New page

'''Unreleased structure'''

The entry 6rgp is ON HOLD

Authors: Polyakov, K.M., Gavryushov, S., Fedorova, T.V., Glazunova, O.A., Popov, A.N.

Description: Single crystal serial study of the X-ray induced enzymatic reduction of molecular oxygen to water for laccase from Steccherinum murashkinskyi at sub-atomic resolution. Second structure of the series with 165 KGy dose.
[[Category: Unreleased Structures]]
[[Category: Fedorova, T.V]]
[[Category: Popov, A.N]]
[[Category: Glazunova, O.A]]
[[Category: Gavryushov, S]]
[[Category: Polyakov, K.M]]