6rhq - Revision history

OCA at 12:20, 24 January 2024

OCA — Wed, 24 Jan 2024 12:20:43 GMT

←Older revision		Revision as of 12:20, 24 January 2024
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	<StructureSection load='6rhq' size='340' side='right'caption='[[6rhq]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='6rhq' size='340' side='right'caption='[[6rhq]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rhq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RHQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RHQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rhq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseoflavus Streptomyces griseoflavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RHQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CU:COPPER+(II)+ION'>CU</scene~~>, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5mkm\|5mkm]], [[6fc7\|6fc7]], [[6fdj\|6fdj]], [[6rh9\|6rh9]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rhq OCA], [https://pdbe.org/6rhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rhq RCSB], [https://www.ebi.ac.uk/pdbsum/6rhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rhq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rhq OCA], [~~http~~://pdbe.org/6rhq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rhq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rhq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rhq ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M4FJ81_9ACTN A0A0M4FJ81_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6rhq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6rhq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Laccase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gabdulkhakov, A G]]
-	[[Category: Kolyadenko, I A]]
-	[[Category: Tishchenko, T V]]
-	[[Category: Oxidoreductase]]
	[[Category: Streptomyces griseoflavus]]		[[Category: Streptomyces griseoflavus]]
-	[[Category: ~~Two-domain laccase~~]]	+	[[Category: Gabdulkhakov AG]]
		+	[[Category: Kolyadenko IA]]
		+	[[Category: Tishchenko TV]]

OCA at 10:52, 17 July 2019

OCA — Wed, 17 Jul 2019 10:52:07 GMT

←Older revision		Revision as of 10:52, 17 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6rhq is ~~ON HOLD~~	+	==Crystal Structure of Two-Domain Laccase mutant I170A from Streptomyces griseoflavus==
		+	<StructureSection load='6rhq' size='340' side='right'caption='[[6rhq]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rhq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RHQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5mkm\|5mkm]], [[6fc7\|6fc7]], [[6fdj\|6fdj]], [[6rh9\|6rh9]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rhq OCA], [http://pdbe.org/6rhq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rhq RCSB], [http://www.ebi.ac.uk/pdbsum/6rhq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rhq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Laccases (EC 1.10.3.2) are multicopper oxidoreductases acting on diphenols and related substances. Laccases are highly important for biotechnology and environmental remediation. These enzymes contain mononuclear one T2 copper ion and two T3 copper ions (Cu3alpha and Cu3beta), which form the so-called trinuclear center (TNC). Along with the typical three-domain laccases Bacteria produce two-domain (2D) enzymes, which are active at neutral and basic pH, thermostable, and resistant to inhibitors. In this work we present the comparative analysis of crystal structures and catalytic properties of recombinant 2D laccase from Streptomyces griseoflavus Ac-993 (SgfSL) and its four mutant forms with replacements of two amino acid residues, located at the narrowing of the presumable T3-solvent tunnels. We obtained inactive enzymes with substitutions of His165, with Phe, and Ile170 with Ala or Phe. His165Ala variant was more active than the wild type. We suggest that His165 is a "gateway" at the O2-tunnel leading from solvent to the Cu3beta of the enzyme. The side chain of Ile170 could be indirectly involved in the coordination of copper ions at the T3 center by maintaining the position of the imidazole ring of His157 that belongs to the first coordination sphere of Cu3alpha.

-	~~Authors:~~ Gabdulkhakov, A~~.G.~~, ~~Tishchenko~~, ~~T.V.~~, ~~Kolyadenko~~, I.A.	+	Investigations of Accessibility of T2/T3 Copper Center of Two-Domain Laccase from Streptomyces griseoflavus Ac-993.,Gabdulkhakov A, Kolyadenko I, Kostareva O, Mikhaylina A, Oliveira P, Tamagnini P, Lisov A, Tishchenko S Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133184. doi: 10.3390/ijms20133184. PMID:31261802<ref>PMID:31261802</ref>

-	~~Description: Crystal Structure~~ of ~~Two~~-~~Domain Laccase mutant I170A from Streptomyces griseoflavus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Tishchenko, T.V~~]]	+	<div class="pdbe-citations 6rhq" style="background-color:#fffaf0;"></div>
-	[[Category: Gabdulkhakov, A.G]]	+	== References ==
-	[[Category: Kolyadenko, I.A]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Laccase]]
		+	[[Category: Large Structures]]
		+	[[Category: Gabdulkhakov, A G]]
		+	[[Category: Kolyadenko, I A]]
		+	[[Category: Tishchenko, T V]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Streptomyces griseoflavus]]
		+	[[Category: Two-domain laccase]]

OCA: Protected "6rhq" [edit=sysop:move=sysop]

OCA — Wed, 01 May 2019 07:22:38 GMT

Protected "6rhq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:22, 1 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rhq is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V., Kolyadenko, I.A. Description: Crystal Structure of Two-Domain Laccase mutant I170A f...

OCA — Wed, 01 May 2019 07:22:36 GMT

New page: '''Unreleased structure''' The entry 6rhq is ON HOLD Authors: Gabdulkhakov, A.G., Tishchenko, T.V., Kolyadenko, I.A. Description: Crystal Structure of Two-Domain Laccase mutant I170A f...

New page

'''Unreleased structure'''

The entry 6rhq is ON HOLD

Authors: Gabdulkhakov, A.G., Tishchenko, T.V., Kolyadenko, I.A.

Description: Crystal Structure of Two-Domain Laccase mutant I170A from Streptomyces griseoflavus
[[Category: Unreleased Structures]]
[[Category: Tishchenko, T.V]]
[[Category: Gabdulkhakov, A.G]]
[[Category: Kolyadenko, I.A]]