6rk4 - Revision history

OCA at 12:22, 24 January 2024

OCA — Wed, 24 Jan 2024 12:22:11 GMT

←Older revision		Revision as of 12:22, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6rk4' size='340' side='right'caption='[[6rk4]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='6rk4' size='340' side='right'caption='[[6rk4]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rk4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_27848 Atcc 27848~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rk4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_simulans Staphylococcus simulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RK4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.43Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1286 ATCC 27848])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk4 OCA], [https://pdbe.org/6rk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rk4 RCSB], [https://www.ebi.ac.uk/pdbsum/6rk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk4 OCA], [~~http~~://pdbe.org/6rk4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rk4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rk4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI]] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.	+	[https://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 27848]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lysostaphin~~]]	+	[[Category: Staphylococcus simulans]]
-	[[Category: Lovering~~, A L~~]]	+	[[Category: Lovering AL]]
-	[[Category: Walters-Morgan, H]]	+	[[Category: Walters-Morgan H]]
-	~~[[Category: Peptide binding protein]]~~	+
-	~~[[Category: Peptidoglycan hydrolase~~]]	+

OCA at 17:56, 20 November 2019

OCA — Wed, 20 Nov 2019 17:56:33 GMT

←Older revision		Revision as of 17:56, 20 November 2019
Line 3:		Line 3:
	<StructureSection load='6rk4' size='340' side='right'caption='[[6rk4]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='6rk4' size='340' side='right'caption='[[6rk4]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rk4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rk4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27848 Atcc 27848]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1286 ATCC 27848])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk4 OCA], [http://pdbe.org/6rk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk4 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk4 OCA], [http://pdbe.org/6rk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk4 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk4 ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI]] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.		[[http://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI]] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lysostaphin is a bacteriolytic enzyme targeting peptidoglycan, the essential component of the bacterial cell envelope. It displays a very potent and specific activity toward staphylococci, including methicillin-resistant Staphylococcus aureus. Lysostaphin causes rapid cell lysis and disrupts biofilms, and is therefore a therapeutic agent of choice to eradicate staphylococcal infections. The C-terminal SH3b domain of lysostaphin recognizes peptidoglycans containing a pentaglycine crossbridge and has been proposed to drive the preferential digestion of staphylococcal cell walls. Here we elucidate the molecular mechanism underpinning recognition of staphylococcal peptidoglycan by the lysostaphin SH3b domain. We show that the pentaglycine crossbridge and the peptide stem are recognized by two independent binding sites located on opposite sides of the SH3b domain, thereby inducing a clustering of SH3b domains. We propose that this unusual binding mechanism allows synergistic and structurally dynamic recognition of S. aureus peptidoglycan and underpins the potent bacteriolytic activity of this enzyme.
		+
		+	Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b.,Gonzalez-Delgado LS, Walters-Morgan H, Salamaga B, Robertson AJ, Hounslow AM, Jagielska E, Sabala I, Williamson MP, Lovering AL, Mesnage S Nat Chem Biol. 2019 Nov 4. pii: 10.1038/s41589-019-0393-4. doi:, 10.1038/s41589-019-0393-4. PMID:31686030<ref>PMID:31686030</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6rk4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 27848]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lysostaphin]]		[[Category: Lysostaphin]]

OCA at 06:00, 16 October 2019

OCA — Wed, 16 Oct 2019 06:00:10 GMT

←Older revision		Revision as of 06:00, 16 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6rk4 is ~~ON HOLD~~	+	==Lysostaphin SH3b P4-G5 complex, synchrotron dataset==
-		+	<StructureSection load='6rk4' size='340' side='right'caption='[[6rk4]], [[Resolution\|resolution]] 1.43Å' scene=''>
-	~~Authors~~: ~~Walters~~-~~Morgan~~, H., ~~Lovering~~, A.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6rk4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK4 FirstGlance]. <br>
-	~~Description~~: ~~Lysostaphin SH3b P4-G5 complex, synchrotron dataset~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K5T:(2~{R})-2-[[(2~{S})-2-[[(4~{R})-5-azanyl-4-[[(2~{S})-2-azanylpropanoyl]amino]-5-oxidanylidene-pentanoyl]amino]-6-[2-[2-[2-[2-(2-azanylethanoylamino)ethanoylamino]ethanoylamino]ethanoylamino]ethanoylamino]hexanoyl]amino]propanoic+acid'>K5T</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>
-	[[Category: Lovering, A.L]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk4 OCA], [http://pdbe.org/6rk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk4 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LSTP_STASI LSTP_STASI]] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lysostaphin]]
		+	[[Category: Lovering, A L]]
	[[Category: Walters-Morgan, H]]		[[Category: Walters-Morgan, H]]
		+	[[Category: Peptide binding protein]]
		+	[[Category: Peptidoglycan hydrolase]]

OCA: Protected "6rk4" [edit=sysop:move=sysop]

OCA — Fri, 10 May 2019 08:25:12 GMT

Protected "6rk4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:25, 10 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rk4 is ON HOLD Authors: Walters-Morgan, H., Lovering, A.L. Description: Lysostaphin SH3b P4-G5 complex, synchrotron dataset [[Category: Unreleased...

OCA — Fri, 10 May 2019 08:25:09 GMT

New page: '''Unreleased structure''' The entry 6rk4 is ON HOLD Authors: Walters-Morgan, H., Lovering, A.L. Description: Lysostaphin SH3b P4-G5 complex, synchrotron dataset [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 6rk4 is ON HOLD

Authors: Walters-Morgan, H., Lovering, A.L.

Description: Lysostaphin SH3b P4-G5 complex, synchrotron dataset
[[Category: Unreleased Structures]]
[[Category: Lovering, A.L]]
[[Category: Walters-Morgan, H]]