6rk7 - Revision history

OCA at 12:22, 24 January 2024

OCA — Wed, 24 Jan 2024 12:22:16 GMT

←Older revision		Revision as of 12:22, 24 January 2024
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	<StructureSection load='6rk7' size='340' side='right'caption='[[6rk7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6rk7' size='340' side='right'caption='[[6rk7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rk7]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ureaplasma_urealyticum_serovar_7_str.~~_atcc_27819~~ Ureaplasma urealyticum serovar 7 str. ~~atcc~~ 27819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RK7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rk7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ureaplasma_urealyticum_serovar_7_str._ATCC_27819 Ureaplasma urealyticum serovar 7 str. ATCC 27819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RK7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[6rjs\|6rjs~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>metK, UUR7_0462 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~519849 Ureaplasma urealyticum serovar 7 str. ATCC 27819])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk7 OCA], [https://pdbe.org/6rk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rk7 RCSB], [https://www.ebi.ac.uk/pdbsum/6rk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk7 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk7 OCA], [~~http~~://pdbe.org/6rk7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rk7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rk7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk7 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6rk7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6rk7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[S-adenosylmethionine synthetase 3D structures\|S-adenosylmethionine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Methionine adenosyltransferase]]~~	+	[[Category: Ureaplasma urealyticum serovar 7 str. ATCC 27819]]
-	[[Category: Ureaplasma urealyticum serovar 7 str. ~~atcc~~ 27819]]	+	[[Category: Bershtein S]]
-	[[Category: Bershtein, S]]	+	[[Category: Kleiner D]]
-	[[Category: Kleiner, D]]	+	[[Category: Shahar A]]
-	[[Category: Shahar, A]]	+	[[Category: Shmulevich F]]
-	[[Category: Shmulevich, F]]	+	[[Category: Zarivach R]]
-	[[Category: Zarivach, R]]	+
-	~~[[Category: Synthetase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:15, 25 December 2019

OCA — Wed, 25 Dec 2019 13:15:09 GMT

←Older revision		Revision as of 13:15, 25 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6rk7 is ~~ON HOLD until Paper~~ Publication	+	==Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum==
		+	<StructureSection load='6rk7' size='340' side='right'caption='[[6rk7]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rk7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ureaplasma_urealyticum_serovar_7_str._atcc_27819 Ureaplasma urealyticum serovar 7 str. atcc 27819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RK7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6rjs\|6rjs]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metK, UUR7_0462 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=519849 Ureaplasma urealyticum serovar 7 str. ATCC 27819])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rk7 OCA], [http://pdbe.org/6rk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rk7 RCSB], [http://www.ebi.ac.uk/pdbsum/6rk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rk7 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methionine S-adenosyltransferases (MATs) are predominantly homotetramers, comprised of dimers of dimers. The larger, highly conserved intradimeric interface harbors two active sites, making the dimer the obligatory functional unit. However, functionality of the smaller, more diverged, and recently evolved interdimeric interface is largely unknown. Here, we show that the interdimeric interface of Ureaplasmaurealiticum MAT has evolved to control the catalytic activity and structural integrity of the homotetramer in response to product accumulation. When all four active sites are occupied with the product, S-adenosylmethionine (SAM), binding of four additional SAM molecules to the interdimeric interface prompts a approximately 45 degrees shift in the dimer orientation and a concomitant approximately 60% increase in the interface area. This rearrangement inhibits the enzymatic activity by locking the flexible active site loops in a closed state and renders the tetramer resistant to proteolytic degradation. Our findings suggest that the interdimeric interface of MATs is subject to rapid evolutionary changes that tailor the molecular properties of the entire homotetramer to the specific needs of the organism.

-	~~Authors: Shahar, A~~., ~~Zarivach~~, R., ~~Bershtein~~, S., ~~Kleiner~~, D., ~~Shmulevich, F~~.	+	The interdimeric interface controls function and stability of Ureaplasma urealiticum methionine S-adenosyltransferase.,Kleiner D, Shmulevich F, Zarivach R, Shahar A, Sharon M, Ben-Nissan G, Bershtein S J Mol Biol. 2019 Dec 6;431(24):4796-4816. doi: 10.1016/j.jmb.2019.09.003. Epub, 2019 Sep 12. PMID:31520601<ref>PMID:31520601</ref>

-	~~Description: Inter-dimeric interface controls function and stability~~ of ~~S-methionine adenosyltransferase from~~ U. ~~urealiticum~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Shmulevich, F~~]]	+	<div class="pdbe-citations 6rk7" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zarivach~~, R]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Methionine adenosyltransferase]]
		+	[[Category: Ureaplasma urealyticum serovar 7 str. atcc 27819]]
		+	[[Category: Bershtein, S]]
	[[Category: Kleiner, D]]		[[Category: Kleiner, D]]
	[[Category: Shahar, A]]		[[Category: Shahar, A]]
-	[[Category: ~~Bershtein~~, S]]	+	[[Category: Shmulevich, F]]
		+	[[Category: Zarivach, R]]
		+	[[Category: Synthetase]]
		+	[[Category: Transferase]]

OCA: Protected "6rk7" [edit=sysop:move=sysop]

OCA — Fri, 10 May 2019 08:26:16 GMT

Protected "6rk7" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:26, 10 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rk7 is ON HOLD until Paper Publication Authors: Shahar, A., Zarivach, R., Bershtein, S., Kleiner, D., Shmulevich, F. Description: Inter-dimeric in...

OCA — Fri, 10 May 2019 08:26:14 GMT

New page: '''Unreleased structure''' The entry 6rk7 is ON HOLD until Paper Publication Authors: Shahar, A., Zarivach, R., Bershtein, S., Kleiner, D., Shmulevich, F. Description: Inter-dimeric in...

New page

'''Unreleased structure'''

The entry 6rk7 is ON HOLD until Paper Publication

Authors: Shahar, A., Zarivach, R., Bershtein, S., Kleiner, D., Shmulevich, F.

Description: Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum
[[Category: Unreleased Structures]]
[[Category: Shmulevich, F]]
[[Category: Zarivach, R]]
[[Category: Kleiner, D]]
[[Category: Shahar, A]]
[[Category: Bershtein, S]]