6rnw - Revision history

OCA at 12:24, 24 January 2024

OCA — Wed, 24 Jan 2024 12:24:43 GMT

←Older revision		Revision as of 12:24, 24 January 2024
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	<StructureSection load='6rnw' size='340' side='right'caption='[[6rnw]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='6rnw' size='340' side='right'caption='[[6rnw]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rnw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thermosynechococcus_elongatus~~ Thermosynechococcus ~~elongatus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RNW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rnw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RNW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.92Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">por ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=146786 Thermosynechococcus elongatus])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Protochlorophyllide_reductase Protochlorophyllide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.33 1.3.1.33]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnw OCA], [https://pdbe.org/6rnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rnw RCSB], [https://www.ebi.ac.uk/pdbsum/6rnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnw OCA], [~~http~~://pdbe.org/6rnw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rnw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rnw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~Q8DLC1_THEEB Q8DLC1_THEEB]~~] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).[RuleBase:RU365001]	+	[https://www.uniprot.org/uniprot/Q8DLC1_THEVB Q8DLC1_THEVB] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).[RuleBase:RU365001]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Protochlorophyllide reductase]]~~	+	[[Category: Thermosynechococcus vestitus]]
-	[[Category: Thermosynechococcus ~~elongatus~~]]	+	[[Category: Levy CW]]
-	[[Category: Levy~~, C W]]~~	+
-	~~[[Category: Light dependent]]~~	+
-	~~[[Category: Photosynthesis~~]]	+

OCA at 08:47, 6 November 2019

OCA — Wed, 06 Nov 2019 08:47:43 GMT

←Older revision		Revision as of 08:47, 6 November 2019
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	<StructureSection load='6rnw' size='340' side='right'caption='[[6rnw]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='6rnw' size='340' side='right'caption='[[6rnw]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rnw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RNW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rnw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RNW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">por ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=146786 Thermosynechococcus elongatus])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protochlorophyllide_reductase Protochlorophyllide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.33 1.3.1.33] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protochlorophyllide_reductase Protochlorophyllide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.33 1.3.1.33] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnw OCA], [http://pdbe.org/6rnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rnw RCSB], [http://www.ebi.ac.uk/pdbsum/6rnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnw OCA], [http://pdbe.org/6rnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rnw RCSB], [http://www.ebi.ac.uk/pdbsum/6rnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnw ProSAT]</span></td></tr>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q8DLC1_THEEB Q8DLC1_THEEB]] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).[RuleBase:RU365001]		[[http://www.uniprot.org/uniprot/Q8DLC1_THEEB Q8DLC1_THEEB]] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).[RuleBase:RU365001]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme protochlorophyllide oxidoreductase (POR) catalyses a light-dependent step in chlorophyll biosynthesis that is essential to photosynthesis and, ultimately, all life on Earth(1-3). POR, which is one of three known light-dependent enzymes(4,5), catalyses reduction of the photosensitizer and substrate protochlorophyllide to form the pigment chlorophyllide. Despite its biological importance, the structural basis for POR photocatalysis has remained unknown. Here we report crystal structures of cyanobacterial PORs from Thermosynechococcus elongatus and Synechocystis sp. in their free forms, and in complex with the nicotinamide coenzyme. Our structural models and simulations of the ternary protochlorophyllide-NADPH-POR complex identify multiple interactions in the POR active site that are important for protochlorophyllide binding, photosensitization and photochemical conversion to chlorophyllide. We demonstrate the importance of active-site architecture and protochlorophyllide structure in driving POR photochemistry in experiments using POR variants and protochlorophyllide analogues. These studies reveal how the POR active site facilitates light-driven reduction of protochlorophyllide by localized hydride transfer from NADPH and long-range proton transfer along structurally defined proton-transfer pathways.
		+
		+	Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis.,Zhang S, Heyes DJ, Feng L, Sun W, Johannissen LO, Liu H, Levy CW, Li X, Yang J, Yu X, Lin M, Hardman SJO, Hoeven R, Sakuma M, Hay S, Leys D, Rao Z, Zhou A, Cheng Q, Scrutton NS Nature. 2019 Oct 23. pii: 10.1038/s41586-019-1685-2. doi:, 10.1038/s41586-019-1685-2. PMID:31645759<ref>PMID:31645759</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6rnw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Protochlorophyllide reductase]]		[[Category: Protochlorophyllide reductase]]
		+	[[Category: Thermosynechococcus elongatus]]
	[[Category: Levy, C W]]		[[Category: Levy, C W]]
	[[Category: Light dependent]]		[[Category: Light dependent]]
	[[Category: Photosynthesis]]		[[Category: Photosynthesis]]

OCA at 09:08, 30 October 2019

OCA — Wed, 30 Oct 2019 09:08:58 GMT

←Older revision		Revision as of 09:08, 30 October 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6rnw is ~~ON HOLD until Paper Publication~~	+	==The crystal structure of Thermosynechococcus elongatus protochlorophyllide oxidoreductase (POR) in complex with NADP.==
-		+	<StructureSection load='6rnw' size='340' side='right'caption='[[6rnw]], [[Resolution\|resolution]] 1.92Å' scene=''>
-	~~Authors~~: ~~Levy~~, C.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6rnw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RNW FirstGlance]. <br>
-	~~Description~~: ~~The crystal structure~~ of ~~Thermosynechococcus elongatus~~ protochlorophyllide ~~oxidoreductase~~ (~~POR~~) ~~in complex with NADP~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protochlorophyllide_reductase Protochlorophyllide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.33 1.3.1.33] </span></td></tr>
-	[[Category: Levy, C.W]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnw OCA], [http://pdbe.org/6rnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rnw RCSB], [http://www.ebi.ac.uk/pdbsum/6rnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8DLC1_THEEB Q8DLC1_THEEB]] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).[RuleBase:RU365001]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Protochlorophyllide reductase]]
		+	[[Category: Levy, C W]]
		+	[[Category: Light dependent]]
		+	[[Category: Photosynthesis]]

OCA at 04:41, 29 May 2019

OCA — Wed, 29 May 2019 04:41:29 GMT

←Older revision		Revision as of 04:41, 29 May 2019
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	Authors: Levy, C.W.		Authors: Levy, C.W.

-	Description: The crystal structure of Thermosynechococcus. elongatus protochlorophyllide oxidoreductase (POR) in complex with ~~NADPH~~.	+	Description: The crystal structure of Thermosynechococcus elongatus protochlorophyllide oxidoreductase (POR) in complex with NADP.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Levy, C.W]]		[[Category: Levy, C.W]]

OCA at 04:14, 22 May 2019

OCA — Wed, 22 May 2019 04:14:40 GMT

←Older revision		Revision as of 04:14, 22 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6rnw is ON HOLD	+	The entry 6rnw is ON HOLD until Paper Publication

	Authors: Levy, C.W.		Authors: Levy, C.W.

OCA: Protected "6rnw" [edit=sysop:move=sysop]

OCA — Tue, 21 May 2019 06:03:15 GMT

Protected "6rnw" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:03, 21 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rnw is ON HOLD Authors: Levy, C.W. Description: The crystal structure of Thermosynechococcus. elongatus protochlorophyllide oxidoreductase (POR) i...

OCA — Tue, 21 May 2019 06:03:13 GMT

New page: '''Unreleased structure''' The entry 6rnw is ON HOLD Authors: Levy, C.W. Description: The crystal structure of Thermosynechococcus. elongatus protochlorophyllide oxidoreductase (POR) i...

New page

'''Unreleased structure'''

The entry 6rnw is ON HOLD

Authors: Levy, C.W.

Description: The crystal structure of Thermosynechococcus. elongatus protochlorophyllide oxidoreductase (POR) in complex with NADPH.
[[Category: Unreleased Structures]]
[[Category: Levy, C.W]]