6rpv - Revision history

OCA at 11:01, 14 June 2023

OCA — Wed, 14 Jun 2023 11:01:35 GMT

←Older revision		Revision as of 11:01, 14 June 2023
Line 1:		Line 1:

	==Extremely stable monomeric variant of human cystatin C with single amino acid substitution==		==Extremely stable monomeric variant of human cystatin C with single amino acid substitution==
-	<StructureSection load='6rpv' size='340' side='right'caption='[[6rpv~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6rpv' size='340' side='right'caption='[[6rpv]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rpv]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RPV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rpv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RPV FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CST3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rpv OCA], [https://pdbe.org/6rpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rpv RCSB], [https://www.ebi.ac.uk/pdbsum/6rpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rpv ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rpv OCA], [~~http~~://pdbe.org/6rpv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rpv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rpv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rpv ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN]] Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:[~~http~~://omim.org/entry/105150 105150]]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.<ref>PMID:2541223</ref> <ref>PMID:1352269</ref> Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:[~~http~~://omim.org/entry/611953 611953]]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.<ref>PMID:11815350</ref>	+	[https://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN] Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:[https://omim.org/entry/105150 105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.<ref>PMID:2541223</ref> <ref>PMID:1352269</ref> Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:[https://omim.org/entry/611953 611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.<ref>PMID:11815350</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN]] As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.	+	[https://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN] As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jurczak, P]]	+	[[Category: Jurczak P]]
-	[[Category: Kozak, M]]	+	[[Category: Kozak M]]
-	[[Category: Maszota-Zieleniak, M]]	+	[[Category: Maszota-Zieleniak M]]
-	[[Category: Rodziewicz-Motowidlo, S]]	+	[[Category: Rodziewicz-Motowidlo S]]
-	[[Category: Zhukov, I]]	+	[[Category: Zhukov I]]
-	~~[[Category: Hcc v57g mutation]]~~	+
-	~~[[Category: Human cystatin c]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein structure~~]]	+

OCA at 06:55, 21 August 2019

OCA — Wed, 21 Aug 2019 06:55:09 GMT

←Older revision		Revision as of 06:55, 21 August 2019
Line 3:		Line 3:
	<StructureSection load='6rpv' size='340' side='right'caption='[[6rpv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='6rpv' size='340' side='right'caption='[[6rpv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rpv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rpv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RPV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rpv OCA], [http://pdbe.org/6rpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rpv RCSB], [http://www.ebi.ac.uk/pdbsum/6rpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rpv ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CST3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rpv OCA], [http://pdbe.org/6rpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rpv RCSB], [http://www.ebi.ac.uk/pdbsum/6rpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rpv ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Jurczak, P]]
		+	[[Category: Kozak, M]]
		+	[[Category: Maszota-Zieleniak, M]]
	[[Category: Rodziewicz-Motowidlo, S]]		[[Category: Rodziewicz-Motowidlo, S]]
	[[Category: Zhukov, I]]		[[Category: Zhukov, I]]

OCA at 06:33, 31 July 2019

OCA — Wed, 31 Jul 2019 06:33:19 GMT

←Older revision		Revision as of 06:33, 31 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6rpv is ~~ON HOLD~~	+	==Extremely stable monomeric variant of human cystatin C with single amino acid substitution==
		+	<StructureSection load='6rpv' size='340' side='right'caption='[[6rpv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rpv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RPV FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rpv OCA], [http://pdbe.org/6rpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rpv RCSB], [http://www.ebi.ac.uk/pdbsum/6rpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rpv ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN]] Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:[http://omim.org/entry/105150 105150]]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.<ref>PMID:2541223</ref> <ref>PMID:1352269</ref> Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:[http://omim.org/entry/611953 611953]]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.<ref>PMID:11815350</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CYTC_HUMAN CYTC_HUMAN]] As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human cystatin C (hCC), a member of the superfamily of papain-like cysteine protease inhibitors, is the most widespread cystatin in human body fluids. This small protein, in addition to its physiological function, is involved in various diseases, including cerebral amyloid angiopathy, cerebral hemorrhage, stroke, and dementia. Physiologically active hCC is a monomer. However, all structural studies based on crystallization led to the dimeric structure formed as a result of a three-dimensional exchange of the protein domains (3D domain swapping). The monomeric structure was obtained only for hCC variant V57N and for the protein stabilized by an additional disulfide bridge. With this study, we extend the number of models of monomeric hCC by an additional hCC variant with a single amino acid substitution in the flexible loop L1. The V57G variant was chosen for the X-ray and NMR structural analysis due to its exceptional conformational stability in solution. In this work we show for the first time the structural and dynamics studies of human cystatin C variant in solution. We were also able to compare these data with the crystal structure of the hCC V57G and with other cystatins. The overall cystatin fold is retained in the solute form. Additionally, structural information concerning the N-terminus was obtained during our studies and presented for the first time. This article is protected by copyright. All rights reserved.

-	~~Authors: Zhukov~~, I., Rodziewicz-Motowidlo, S.	+	NMR and crystallographic structural studies of the extremely stable monomeric variant of human cystatin C with single amino acid substitution.,Maszota-Zieleniak M, Jurczak P, Orlikowska M, Zhukov I, Borek D, Otwinowski Z, Skowron P, Pietralik Z, Kozak M, Szymanska A, Rodziewicz-Motowidlo S FEBS J. 2019 Jul 22. doi: 10.1111/febs.15010. PMID:31330077<ref>PMID:31330077</ref>

-	~~Description: Extremely stable monomeric variant~~ of ~~human cystatin C with single amino acid substitution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Zhukov, I~~]]	+	<div class="pdbe-citations 6rpv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rodziewicz-Motowidlo, S]]		[[Category: Rodziewicz-Motowidlo, S]]
		+	[[Category: Zhukov, I]]
		+	[[Category: Hcc v57g mutation]]
		+	[[Category: Human cystatin c]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein structure]]

OCA: Protected "6rpv" [edit=sysop:move=sysop]

OCA — Wed, 22 May 2019 04:20:29 GMT

Protected "6rpv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rpv is ON HOLD Authors: Zhukov, I., Rodziewicz-Motowidlo, S. Description: Extremely stable monomeric variant of human cystatin C with single amino...

OCA — Wed, 22 May 2019 04:20:28 GMT

New page: '''Unreleased structure''' The entry 6rpv is ON HOLD Authors: Zhukov, I., Rodziewicz-Motowidlo, S. Description: Extremely stable monomeric variant of human cystatin C with single amino...

New page

'''Unreleased structure'''

The entry 6rpv is ON HOLD

Authors: Zhukov, I., Rodziewicz-Motowidlo, S.

Description: Extremely stable monomeric variant of human cystatin C with single amino acid substitution
[[Category: Unreleased Structures]]
[[Category: Zhukov, I]]
[[Category: Rodziewicz-Motowidlo, S]]