6rqq - Revision history

OCA at 12:26, 24 January 2024

OCA — Wed, 24 Jan 2024 12:26:35 GMT

←Older revision		Revision as of 12:26, 24 January 2024
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	<StructureSection load='6rqq' size='340' side='right'caption='[[6rqq]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='6rqq' size='340' side='right'caption='[[6rqq]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rqq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RQQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rqq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RQQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FMT:FORMIC+ACID'>FMT</scene~~>, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.276Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CA9, G250, MN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rqq OCA], [https://pdbe.org/6rqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rqq RCSB], [https://www.ebi.ac.uk/pdbsum/6rqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rqq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rqq OCA], [~~http~~://pdbe.org/6rqq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rqq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rqq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rqq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAH9_HUMAN CAH9_HUMAN]] Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.<ref>PMID:18703501</ref>	+	[https://www.uniprot.org/uniprot/CAH9_HUMAN CAH9_HUMAN] Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.<ref>PMID:18703501</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6rqq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6rqq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Carbonate dehydratase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fisher, Z]]	+	[[Category: Fisher Z]]
-	[[Category: Koruza, K]]	+	[[Category: Koruza K]]
-	~~[[Category: Ca ix]]~~	+
-	~~[[Category: Carbonic anhydrase]]~~	+
-	~~[[Category: Proton transport]]~~	+
-	~~[[Category: Surface variant~~]]	+

OCA at 07:42, 16 October 2019

OCA — Wed, 16 Oct 2019 07:42:15 GMT

←Older revision		Revision as of 07:42, 16 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6rqq is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV.==
		+	<StructureSection load='6rqq' size='340' side='right'caption='[[6rqq]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RQQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA9, G250, MN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rqq OCA], [http://pdbe.org/6rqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rqq RCSB], [http://www.ebi.ac.uk/pdbsum/6rqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rqq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAH9_HUMAN CAH9_HUMAN]] Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.<ref>PMID:18703501</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human carbonic anhydrase IX (CA IX) expression is upregulated in hypoxic solid tumours, promoting cell survival and metastasis. This observation has made CA IX a target for the development of CA isoform-selective inhibitors. To enable structural studies of CA IX-inhibitor complexes using X-ray and neutron crystallography, a CA IX surface variant (CA IXSV; the catalytic domain with six surface amino-acid substitutions) has been developed that can be routinely crystallized. Here, the preparation of protiated (H/H), H/D-exchanged (H/D) and deuterated (D/D) CA IXSV for crystallographic studies and their structural comparison are described. Four CA IXSV X-ray crystal structures are compared: two H/H crystal forms, an H/D crystal form and a D/D crystal form. The overall active-site organization in each version is essentially the same, with only minor positional changes in active-site solvent, which may be owing to deuteration and/or resolution differences. Analysis of the crystal contacts and packing reveals different arrangements of CA IXSV compared with previous reports. To our knowledge, this is the first report comparing three different deuterium-labelled crystal structures of the same protein, marking an important step in validating the active-site structure of CA IXSV for neutron protein crystallography.

-	~~Authors: Fisher~~, Z., Koruza, K.	+	Structural comparison of protiated, H/D-exchanged and deuterated human carbonic anhydrase IX.,Koruza K, Lafumat B, Nyblom M, Mahon BP, Knecht W, McKenna R, Fisher SZ Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):895-903. doi:, 10.1107/S2059798319010027. Epub 2019 Aug 22. PMID:31588921<ref>PMID:31588921</ref>

-	~~Description: X-ray crystal structure~~ of ~~protiated (H) large monoclinic unit cell CA IX SV~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Koruza, K~~]]	+	<div class="pdbe-citations 6rqq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Carbonate dehydratase]]
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Fisher, Z]]		[[Category: Fisher, Z]]
		+	[[Category: Koruza, K]]
		+	[[Category: Ca ix]]
		+	[[Category: Carbonic anhydrase]]
		+	[[Category: Proton transport]]
		+	[[Category: Surface variant]]

OCA at 21:17, 5 June 2019

OCA — Wed, 05 Jun 2019 21:17:18 GMT

←Older revision		Revision as of 21:17, 5 June 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6rqq is ON HOLD	+	The entry 6rqq is ON HOLD until Paper Publication

	Authors: Fisher, Z., Koruza, K.		Authors: Fisher, Z., Koruza, K.
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	Description: X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV.		Description: X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Fisher, Z]]
	[[Category: Koruza, K]]		[[Category: Koruza, K]]
		+	[[Category: Fisher, Z]]

OCA: Protected "6rqq" [edit=sysop:move=sysop]

OCA — Wed, 22 May 2019 04:22:25 GMT

Protected "6rqq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:22, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6rqq is ON HOLD Authors: Fisher, Z., Koruza, K. Description: X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV. [[Catego...

OCA — Wed, 22 May 2019 04:22:23 GMT

New page: '''Unreleased structure''' The entry 6rqq is ON HOLD Authors: Fisher, Z., Koruza, K. Description: X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV. [[Catego...

New page

'''Unreleased structure'''

The entry 6rqq is ON HOLD

Authors: Fisher, Z., Koruza, K.

Description: X-ray crystal structure of protiated (H) large monoclinic unit cell CA IX SV.
[[Category: Unreleased Structures]]
[[Category: Fisher, Z]]
[[Category: Koruza, K]]