6ruz - Revision history

OCA at 12:29, 24 January 2024

OCA — Wed, 24 Jan 2024 12:29:21 GMT

←Older revision		Revision as of 12:29, 24 January 2024
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	<StructureSection load='6ruz' size='340' side='right'caption='[[6ruz]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6ruz' size='340' side='right'caption='[[6ruz]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ruz]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RUZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ruz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RUZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=COA:COENZYME+A'>COA</scene~~>, ~~<scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TT_C1484 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=274 "Flavobacterium thermophilum" Yoshida and Oshima 1971])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ruz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ruz OCA], [~~http~~://pdbe.org/6ruz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ruz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ruz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ruz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ruz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ruz OCA], [https://pdbe.org/6ruz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ruz RCSB], [https://www.ebi.ac.uk/pdbsum/6ruz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ruz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q72HK3_THET2 Q72HK3_THET2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6ruz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ruz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Coenzyme A-Disulfide Reductase\|Coenzyme A-Disulfide Reductase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Koepke, J~~]]	+	[[Category: Thermus thermophilus]]
-	[[Category: ~~Preu,~~ J]]	+	[[Category: Koepke J]]
-	[[Category: ~~Coenzyme a disulfide reductase]]~~	+	[[Category: Preu J]]
-	~~[[Category: Nadh:menaquinone oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:10, 23 October 2019

OCA — Wed, 23 Oct 2019 08:10:46 GMT

←Older revision		Revision as of 08:10, 23 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ruz is ~~ON HOLD until Paper~~ Publication	+	==NADH-dependent Coenzyme A Disulfide Reductase==
		+	<StructureSection load='6ruz' size='340' side='right'caption='[[6ruz]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ruz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RUZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TT_C1484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 "Flavobacterium thermophilum" Yoshida and Oshima 1971])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ruz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ruz OCA], [http://pdbe.org/6ruz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ruz RCSB], [http://www.ebi.ac.uk/pdbsum/6ruz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ruz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the enzyme previously characterized as a type-2 NADH:menaquinone oxidoreductase (NDH-2) from Thermus thermophilus has been solved at a resolution of 2.9A and revealed that this protein is, in fact, a coenzyme A-disulfide reductase (CoADR). Coenzyme A (CoASH) replaces glutathione as the major low molecular weight thiol in Thermus thermophilus and is maintained in the reduced state by this enzyme (CoADR). Although the enzyme does exhibit NADH:menadione oxidoreductase activity expected for NDH-2 enzymes, the specific activity with CoAD as an electron acceptor is about 5-fold higher than with menadione. Furthermore, the crystal structure contains coenzyme A covalently linked Cys44, a catalytic intermediate (Cys44-S-S-CoA) reduced by NADH via the FAD cofactor. Soaking the crystals with menadione shows that menadione can bind to a site near the redox active FAD, consistent with the observed NADH:menadione oxidoreductase activity. CoADRs from other species were also examined and shown to have measurable NADH:menadione oxidoreductase activity. Although a common feature of this family of enzymes, no biological relevance is proposed. The CoADR from T. thermophilus is a soluble homodimeric enzyme. Expression of the recombinant TtCoADR at high levels in E. coli results in a small fraction that co-purifies with the membrane fraction, which was used previously to isolate the enzyme wrongly identified as a membrane-bound NDH-2. It is concluded that T. thermophilus does not contain an authentic NDH-2 component in its aerobic respiratory chain.

-	~~Authors~~:	+	Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus.,Lencina AM, Koepke J, Preu J, Muenke C, Gennis RB, Michel H, Schurig-Briccio LA Biochim Biophys Acta Bioenerg. 2019 Sep 11;1860(11):148080. doi:, 10.1016/j.bbabio.2019.148080. PMID:31520616<ref>PMID:31520616</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6ruz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
		+	[[Category: Large Structures]]
		+	[[Category: Koepke, J]]
		+	[[Category: Preu, J]]
		+	[[Category: Coenzyme a disulfide reductase]]
		+	[[Category: Nadh:menaquinone oxidoreductase]]
		+	[[Category: Oxidoreductase]]

OCA at 04:11, 12 June 2019

OCA — Wed, 12 Jun 2019 04:11:15 GMT

←Older revision		Revision as of 04:11, 12 June 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ruz is ON HOLD	+	The entry 6ruz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ruz" [edit=sysop:move=sysop]

OCA — Wed, 05 Jun 2019 21:22:29 GMT

Protected "6ruz" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:22, 5 June 2019

OCA: New page: '''Unreleased structure''' The entry 6ruz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Jun 2019 21:22:27 GMT

New page: '''Unreleased structure''' The entry 6ruz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ruz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]