6rv8 - Revision history

OCA at 12:29, 24 January 2024

OCA — Wed, 24 Jan 2024 12:29:35 GMT

←Older revision		Revision as of 12:29, 24 January 2024
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	<StructureSection load='6rv8' size='340' side='right'caption='[[6rv8]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6rv8' size='340' side='right'caption='[[6rv8]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rv8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RV8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RV8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rv8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrena_unicolor Cerrena unicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RV8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"><~~scene name~~='~~pdbligand~~=~~BXP~~:~~4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'~~>~~BXP~~</~~scene~~>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCV:4-O-METHYL-ALPHA-D-GLUCURONIC+ACID'>GCV</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6rtv\|6rtv]], [[6ru1\|6ru1]], [[6ru2\|6ru2]], [[6rv7\|6rv7]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCV:4-O-METHYL-ALPHA-D-GLUCURONIC+ACID'>GCV</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900116:4beta-beta-xylobiose'>PRD_900116</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rv8 OCA], [~~http~~://pdbe.org/6rv8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rv8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rv8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rv8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rv8 OCA], [https://pdbe.org/6rv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rv8 RCSB], [https://www.ebi.ac.uk/pdbsum/6rv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rv8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref>	+	[https://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6rv8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6rv8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methylesterase 3D structures\|Methylesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cerrena unicolor]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agger~~, J W~~]]	+	[[Category: Agger JW]]
-	[[Category: Ernst~~, H A~~]]	+	[[Category: Ernst HA]]
-	[[Category: Langkilde, A]]	+	[[Category: Langkilde A]]
-	[[Category: Larsen, S]]	+	[[Category: Larsen S]]
-	[[Category: Meyer, A]]	+	[[Category: Meyer A]]
-	[[Category: Mosbech, C]]	+	[[Category: Mosbech C]]
-	[[Category: Westh, P]]	+	[[Category: Westh P]]
-	~~[[Category: Alpha/beta-hydrolase]]~~	+
-	~~[[Category: Ce15]]~~	+
-	~~[[Category: Esterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ligand-bound~~]]	+

OCA at 09:50, 18 March 2020

OCA — Wed, 18 Mar 2020 09:50:13 GMT

←Older revision		Revision as of 09:50, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6rv8 is ~~ON HOLD~~	+	==Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor covalent complex with the aldouronic acid UXXR==
		+	<StructureSection load='6rv8' size='340' side='right'caption='[[6rv8]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rv8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RV8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RV8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BXP:4-O-BETA-D-XYLOPYRANOSYL-BETA-D-XYLOPYRANOSE'>BXP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCV:4-O-METHYL-ALPHA-D-GLUCURONIC+ACID'>GCV</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6rtv\|6rtv]], [[6ru1\|6ru1]], [[6ru2\|6ru2]], [[6rv7\|6rv7]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rv8 OCA], [http://pdbe.org/6rv8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rv8 RCSB], [http://www.ebi.ac.uk/pdbsum/6rv8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rv8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structural and functional studies were conducted of the glucuronoyl esterase (GE) from Cerrena unicolor (CuGE), an enzyme catalyzing cleavage of lignin-carbohydrate ester bonds. CuGE is an alpha/beta-hydrolase belonging to carbohydrate esterase family 15 (CE15). The enzyme is modular, comprised of a catalytic and a carbohydrate-binding domain. SAXS data show CuGE as an elongated rigid molecule where the two domains are connected by a rigid linker. Detailed structural information of the catalytic domain in its apo- and inactivated form and complexes with aldouronic acids reveal well-defined binding of the 4-O-methyl-a-D-glucuronoyl moiety, not influenced by the nature of the attached xylo-oligosaccharide. Structural and sequence comparisons within CE15 enzymes reveal two distinct structural subgroups. CuGE belongs to the group of fungal CE15-B enzymes with an open and flat substrate-binding site. The interactions between CuGE and its natural substrates are explained and rationalized by the structural results, microscale thermophoresis and isothermal calorimetry.

-	~~Authors~~:	+	The structural basis of fungal glucuronoyl esterase activity on natural substrates.,Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S Nat Commun. 2020 Feb 24;11(1):1026. doi: 10.1038/s41467-020-14833-9. PMID:32094331<ref>PMID:32094331</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6rv8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Agger, J W]]
		+	[[Category: Ernst, H A]]
		+	[[Category: Langkilde, A]]
		+	[[Category: Larsen, S]]
		+	[[Category: Meyer, A]]
		+	[[Category: Mosbech, C]]
		+	[[Category: Westh, P]]
		+	[[Category: Alpha/beta-hydrolase]]
		+	[[Category: Ce15]]
		+	[[Category: Esterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Ligand-bound]]

OCA: Protected "6rv8" [edit=sysop:move=sysop]

OCA — Wed, 12 Jun 2019 04:11:52 GMT

Protected "6rv8" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6rv8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Jun 2019 04:11:51 GMT

New page: '''Unreleased structure''' The entry 6rv8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6rv8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]