OCA at 12:29, 24 January 2024

OCA — Wed, 24 Jan 2024 12:29:53 GMT

←Older revision		Revision as of 12:29, 24 January 2024
Line 1:		Line 1:

	==Crystal structure of the Burkholderia Lethal Factor 1 (BLF1)==		==Crystal structure of the Burkholderia Lethal Factor 1 (BLF1)==
-	<StructureSection load='6rvu' size='340' side='right'caption='[[6rvu]]' scene=''>	+	<StructureSection load='6rvu' size='340' side='right'caption='[[6rvu]], [[Resolution\|resolution]] 0.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rvu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RVU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rvu OCA], [https://pdbe.org/6rvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rvu RCSB], [https://www.ebi.ac.uk/pdbsum/6rvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rvu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.99Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rvu OCA], [https://pdbe.org/6rvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rvu RCSB], [https://www.ebi.ac.uk/pdbsum/6rvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rvu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q63UP7_BURPS Q63UP7_BURPS]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Burkholderia pseudomallei lethal factor 1 (BLF1) exhibits site-specific glutamine deamidase activity against the eukaryotic RNA helicase, eIF4A, thereby blocking mammalian protein synthesis. The structure of a complex between BLF1 C94S and human eIF4A shows that the toxin binds in the cleft between the two RecA-like eIF4A domains forming interactions with residues from both and with the scissile amide of the target glutamine, Gln339, adjacent to the toxin active site. The RecA-like domains adopt a radically twisted orientation compared to other eIF4A structures and the nature and position of conserved residues suggests this may represent a conformation associated with RNA binding. Comparison of the catalytic site of BLF1 with other deamidases and cysteine proteases reveals that they fall into two classes, related by pseudosymmetry, that present either the re or si faces of the target amide/peptide to the nucleophilic sulfur, highlighting constraints in the convergent evolution of their Cys-His active sites.
		+
		+	Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1.,Mobbs GW, Aziz AA, Dix SR, Blackburn GM, Sedelnikova SE, Minshull TC, Dickman MJ, Baker PJ, Nathan S, Raih MF, Rice DW Commun Biol. 2022 Mar 28;5(1):272. doi: 10.1038/s42003-022-03186-2. PMID:35347220<ref>PMID:35347220</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6rvu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burkholderia pseudomallei K96243]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Aziz AA]]		[[Category: Aziz AA]]

OCA at 11:44, 27 April 2022

OCA — Wed, 27 Apr 2022 11:44:01 GMT

←Older revision		Revision as of 11:44, 27 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6rvu is ~~ON HOLD~~	+	==Crystal structure of the Burkholderia Lethal Factor 1 (BLF1)==
-		+	<StructureSection load='6rvu' size='340' side='right'caption='[[6rvu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RVU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rvu OCA], [https://pdbe.org/6rvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rvu RCSB], [https://www.ebi.ac.uk/pdbsum/6rvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rvu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Aziz AA]]
		+	[[Category: Baker PJ]]
		+	[[Category: Blackburn GM]]
		+	[[Category: Dickman MJ]]
		+	[[Category: Firdaus-Raih M]]
		+	[[Category: Minshull TC]]
		+	[[Category: Mobbs GW]]
		+	[[Category: Nathan S]]
		+	[[Category: Rice DW]]
		+	[[Category: Sedelnikova SE]]

OCA: Protected "6rvu" [edit=sysop:move=sysop]

OCA — Wed, 12 Jun 2019 04:13:16 GMT

Protected "6rvu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6rvu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Jun 2019 04:13:14 GMT

New page: '''Unreleased structure''' The entry 6rvu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6rvu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6rvu - Revision history

OCA at 12:29, 24 January 2024

OCA at 11:44, 27 April 2022

OCA: Protected "6rvu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6rvu is ON HOLD Authors: Description: Category: Unreleased Structures