6rxf - Revision history

OCA at 12:30, 24 January 2024

OCA — Wed, 24 Jan 2024 12:30:40 GMT

←Older revision		Revision as of 12:30, 24 January 2024
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-	====	+	==Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate==
-	<StructureSection load='6rxf' size='340' side='right'caption='[[6rxf]]' scene=''>	+	<StructureSection load='6rxf' size='340' side='right'caption='[[6rxf]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rxf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis_ATCC_15697_=_JCM_1222_=_DSM_20088 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RXF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxf OCA], [https://pdbe.org/6rxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rxf RCSB], [https://www.ebi.ac.uk/pdbsum/6rxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.397Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NEP:N1-PHOSPHONOHISTIDINE'>NEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxf OCA], [https://pdbe.org/6rxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rxf RCSB], [https://www.ebi.ac.uk/pdbsum/6rxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B7GTV0_BIFLS B7GTV0_BIFLS]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Highly engineered phytases, which sequentially hydrolyze the hexakisphosphate ester of inositol known as phytic acid, are routinely added to the feeds of monogastric animals to improve phosphate bioavailability. New phytases are sought as starting points to further optimize the rate and extent of dephosphorylation of phytate in the animal digestive tract. Multiple inositol polyphosphate phosphatases (MINPPs) are clade 2 histidine phosphatases (HP2P) able to carry out the stepwise hydrolysis of phytate. MINPPs are not restricted by a strong positional specificity making them attractive targets for development as feed enzymes. Here, we describe the characterization of a MINPP from the Gram-positive bacterium Bifidobacterium longum (BlMINPP). BlMINPP has a typical HP2P-fold but, unusually, possesses a large alpha-domain polypeptide insertion relative to other MINPPs. This insertion, termed the U-loop, spans the active site and contributes to substrate specificity pockets underpopulated in other HP2Ps. Mutagenesis of U-loop residues reveals its contribution to enzyme kinetics and thermostability. Moreover, four crystal structures of the protein along the catalytic cycle capture, for the first time in an HP2P, a large ligand-driven alpha-domain motion essential to allow substrate access to the active site. This motion recruits residues both downstream of a molecular hinge and on the U-loop to participate in specificity subsites, and mutagenesis identified a mobile lysine residue as a key determinant of positional specificity of the enzyme. Taken together, these data provide important new insights to the factors determining stability, substrate recognition, and the structural mechanism of hydrolysis in this industrially important group of enzymes.
		+
		+	Snapshots during the catalytic cycle of a histidine acid phytase reveal an induced-fit structural mechanism.,Acquistapace IM, Zi Etek MA, Li AWH, Salmon M, Kuhn I, Bedford MR, Brearley CA, Hemmings AM J Biol Chem. 2020 Dec 18;295(51):17724-17737. doi: 10.1074/jbc.RA120.015925. PMID:33454010<ref>PMID:33454010</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6rxf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Acquistapace IM]]
		+	[[Category: Brearley CA]]
		+	[[Category: Hemmings AM]]

OCA at 06:45, 24 February 2021

OCA — Wed, 24 Feb 2021 06:45:36 GMT

←Older revision		Revision as of 06:45, 24 February 2021
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-	==~~Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate~~==	+	====
	<StructureSection load='6rxf' size='340' side='right'caption='[[6rxf]]' scene=''>		<StructureSection load='6rxf' size='340' side='right'caption='[[6rxf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~6RXF~~ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=~~6RXF~~ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6rxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxf OCA], [~~http~~://pdbe.org/6rxf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rxf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rxf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxf ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxf OCA], [https://pdbe.org/6rxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rxf RCSB], [https://www.ebi.ac.uk/pdbsum/6rxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxf ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Acquistapace IM]]~~	+	[[Category: Z-disk]]
-	~~[[Category: Brearley CA]]~~	+
-	~~[[Category: Hemmings AM~~]]	+

OCA at 14:03, 8 July 2020

OCA — Wed, 08 Jul 2020 14:03:18 GMT

←Older revision		Revision as of 14:03, 8 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6rxf ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate==
-		+	<StructureSection load='6rxf' size='340' side='right'caption='[[6rxf]]' scene=''>
-	~~Authors~~: ~~Acquistapace~~, I.M., ~~Brearley~~, C.A., ~~Hemmings~~, A.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RXF FirstGlance]. <br>
-	~~Description: Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxf OCA], [http://pdbe.org/6rxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rxf RCSB], [http://www.ebi.ac.uk/pdbsum/6rxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Brearley, C.A~~]]	+	__TOC__
-	[[Category: ~~Acquistapace, I.M~~]]	+	</StructureSection>
-	[[Category: Hemmings~~, A.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Acquistapace IM]]
		+	[[Category: Brearley CA]]
		+	[[Category: Hemmings AM]]

OCA at 04:21, 10 July 2019

OCA — Wed, 10 Jul 2019 04:21:42 GMT

←Older revision		Revision as of 04:21, 10 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6rxf is ON HOLD	+	The entry 6rxf is ON HOLD until Paper Publication

	Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.		Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.

OCA: Protected "6rxf" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:29:29 GMT

Protected "6rxf" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:29, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6rxf is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Crystal Structure of Bifidobacterium longum Multiple Inosi...

OCA — Wed, 19 Jun 2019 04:29:28 GMT

New page: '''Unreleased structure''' The entry 6rxf is ON HOLD Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M. Description: Crystal Structure of Bifidobacterium longum Multiple Inosi...

New page

'''Unreleased structure'''

The entry 6rxf is ON HOLD

Authors: Acquistapace, I.M., Brearley, C.A., Hemmings, A.M.

Description: Crystal Structure of Bifidobacterium longum Multiple Inositol Polyphosphate Phosphatase Phosphohistidine Intermediate
[[Category: Unreleased Structures]]
[[Category: Brearley, C.A]]
[[Category: Acquistapace, I.M]]
[[Category: Hemmings, A.M]]