6rzn - Revision history

OCA at 12:31, 24 January 2024

OCA — Wed, 24 Jan 2024 12:31:59 GMT

←Older revision		Revision as of 12:31, 24 January 2024
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	<StructureSection load='6rzn' size='340' side='right'caption='[[6rzn]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='6rzn' size='340' side='right'caption='[[6rzn]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6rzn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Uncultivated_bacterium Uncultivated~~ bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RZN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6RZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6rzn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RZN FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase~~]~~, with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~1.~~1.73 3.1.1.73] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.907Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6rzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rzn OCA], [~~http~~://pdbe.org/6rzn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6rzn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6rzn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6rzn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rzn OCA], [https://pdbe.org/6rzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rzn RCSB], [https://www.ebi.ac.uk/pdbsum/6rzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rzn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Feruloyl esterase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Uncultivated~~ bacterium]]	+	[[Category: Uncultured bacterium]]
-	[[Category: Fredslund, F]]	+	[[Category: Fredslund F]]
-	[[Category: Welner~~, D H~~]]	+	[[Category: Welner DH]]
-	[[Category: Wilkens, C]]	+	[[Category: Wilkens C]]
-	~~[[Category: Carbohydrate binding module family 48]]~~	+
-	~~[[Category: Carbohydrate esterase family 1]]~~	+
-	~~[[Category: Cbm48]]~~	+
-	~~[[Category: Ce1]]~~	+
-	~~[[Category: Ferulic acid esterease]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:26, 10 October 2019

OCA — Thu, 10 Oct 2019 06:26:21 GMT

←Older revision		Revision as of 06:26, 10 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6rzn is ~~ON HOLD~~	+	==Crystal structure of the N-terminal carbohydrate binding module family 48 and ferulic acid esterase from the multi-enzyme CE1-GH62-GH10==
		+	<StructureSection load='6rzn' size='340' side='right'caption='[[6rzn]], [[Resolution\|resolution]] 1.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6rzn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RZN FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rzn OCA], [http://pdbe.org/6rzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rzn RCSB], [http://www.ebi.ac.uk/pdbsum/6rzn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rzn ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Feruloyl esterases (EC 3.1.1.73), belonging to carbohydrate esterase family 1 (CE1), hydrolyze ester bonds between ferulic acid (FA) and arabinose moieties in arabinoxylans. Recently, some CE1 enzymes identified in metagenomics studies have been predicted to contain a family 48 carbohydrate-binding module (CBM48), a CBM family associated with starch binding. Two of these CE1s, wtsFae1A and wtsFae1B isolated from wastewater treatment surplus sludge, have a cognate CBM48 domain and are feruloyl esterases, and wtsFae1A binds arabinoxylan. Here, we show that wtsFae1B also binds to arabinoxylan and that neither binds starch. Surface plasmon resonance analysis revealed that wtsFae1B's Kd for xylohexaose is 14.8 muM and that it does not bind to starch mimics, beta-cyclodextrin, or maltohexaose. Interestingly, in the absence of CBM48 domains, the CE1 regions from wtsFae1A and wtsFae1B did not bind arabinoxylan and were also unable to catalyze FA release from arabinoxylan. Pretreatment with a beta-D-1,4-xylanase did enable CE1 domain-mediated FA release from arabinoxylan in the absence of CBM48, indicating that CBM48 is essential for the CE1 activity on the polysaccharide. Crystal structures of wtsFae1A (at 1.63 A resolution) and wtsFae1B (1.98 A) revealed that both are folded proteins comprising structurally conserved hydrogen bonds that lock the CBM48 position relative to that of the CE1 domain. wtsFae1A docking indicated that both enzymes accommodate the arabinoxylan backbone in a cleft at the CE1-CBM48 domain interface. Binding at this cleft appears to enable CE1 activities on polymeric arabinoxylan, illustrating an unexpected and crucial role of CBM48 domains for accommodating arabinoxylan.

-	~~Authors~~:	+	A carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylan.,Holck J, Fredslund F, Moller MS, Brask J, Krogh KBRM, Lange L, Welner DH, Svensson B, Meyer AS, Wilkens C J Biol Chem. 2019 Sep 26. pii: RA119.009523. doi: 10.1074/jbc.RA119.009523. PMID:31558605<ref>PMID:31558605</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6rzn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Feruloyl esterase]]
		+	[[Category: Large Structures]]
		+	[[Category: Uncultivated bacterium]]
		+	[[Category: Fredslund, F]]
		+	[[Category: Welner, D H]]
		+	[[Category: Wilkens, C]]
		+	[[Category: Carbohydrate binding module family 48]]
		+	[[Category: Carbohydrate esterase family 1]]
		+	[[Category: Cbm48]]
		+	[[Category: Ce1]]
		+	[[Category: Ferulic acid esterease]]
		+	[[Category: Hydrolase]]

OCA: Protected "6rzn" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:35:10 GMT

Protected "6rzn" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6rzn is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Jun 2019 04:35:08 GMT

New page: '''Unreleased structure''' The entry 6rzn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6rzn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]