OCA at 10:30, 26 February 2020

OCA — Wed, 26 Feb 2020 10:30:37 GMT

←Older revision		Revision as of 10:30, 26 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6s5a is ~~ON HOLD~~	+	==CRYSTAL STRUCTURE OF FC P329G LALA WITH ANTI FC P329G FAB==
		+	<StructureSection load='6s5a' size='340' side='right'caption='[[6s5a]], [[Resolution\|resolution]] 1.72Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s5a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S5A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s5a OCA], [http://pdbe.org/6s5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s5a RCSB], [http://www.ebi.ac.uk/pdbsum/6s5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s5a ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monoclonal antibody-based therapeutics are an integral part of treatment of different human diseases, and the selection of suitable antibody candidates during the discovery phase is essential. Here, we describe a novel, cellular screening approach for the identification and characterization of therapeutic antibodies suitable for conversion into T cell bispecific antibodies using chimeric antigen receptor (CAR) transduced Jurkat-NFAT-luciferase reporter cells (CAR-J). For that purpose, we equipped a Jurkat-NFAT reporter cell line with a universal CAR, based on a monoclonal antibody recognizing the P329G mutation in the Fc-part of effector-silenced human IgG1-antibodies. In addition to scFv-based second generation CARs, Fab-based CARs employing the P329G-binder were generated. Using these anti-P329G-CAR-J cells together with the respective P329G-mutated IgG1-antibodies, we established a system, which facilitates the rapid testing of therapeutic antibody candidates in a flexible, high throughput setting during early stage discovery. We show that both, scFv- and Fab-based anti-P329G-CAR-J cells elicit a robust and dose-dependent luciferase signal if the respective antibody acts as an adaptor between tumor target and P329G-CAR-J cells. Importantly, we could demonstrate that functional characteristics of the antibody candidates, derived from the anti-P329G-CAR-J screening assay, are predictive for the functionality of these antibodies in the T cell bispecific antibody format.

-	~~Authors~~: ~~Ehler, A~~., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., ~~Birk, M.~~, Freimoser-Grundschober, A~~., Bruenker~~, P~~., Moessner~~, E., Umana, P., Kobold, S., Klein, C.	+	P329G-CAR-J: a novel Jurkat-NFAT-based CAR-T reporter system recognizing the P329G Fc mutation.,Darowski D, Jost C, Stubenrauch K, Wessels U, Benz J, Ehler A, Freimoser-Grundschober A, Brunker P, Mossner E, Umana P, Kobold S, Klein C Protein Eng Des Sel. 2019 Dec 31;32(5):207-218. doi: 10.1093/protein/gzz027. PMID:31504896<ref>PMID:31504896</ref>

-	~~Description~~: ~~CRYSTAL STRUCTURE OF FC P329G LALA WITH ANTI FC P329G FAB~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Kobold~~, S]]	+	<div class="pdbe-citations 6s5a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
		+	[[Category: Benz, J]]
		+	[[Category: Birk, M]]
		+	[[Category: Bruenker, P]]
		+	[[Category: Darowski, D]]
	[[Category: Ehler, A]]		[[Category: Ehler, A]]
-	[[Category: ~~Wessels~~, ~~U]]~~	+	[[Category: Freimoser-Grundschober, A]]
-	~~[[Category: Klein, C~~]]	+
	[[Category: Jost, C]]		[[Category: Jost, C]]
		+	[[Category: Klein, C]]
		+	[[Category: Kobold, S]]
	[[Category: Moessner, E]]		[[Category: Moessner, E]]
-	[[Category: Umana, P]]
-	[[Category: Birk, M]]
-	[[Category: Freimoser-Grundschober, A]]
	[[Category: Stubenrauch, K]]		[[Category: Stubenrauch, K]]
-	[[Category: ~~Benz~~, J]]	+	[[Category: Umana, P]]
-	[[Category: ~~Darowski~~, D]]	+	[[Category: Wessels, U]]
-	[[Category: ~~Bruenker, P~~]]	+	[[Category: Anti p329g lala fab]]
		+	[[Category: Antibody]]
		+	[[Category: Fab]]
		+	[[Category: Fc]]
		+	[[Category: Immune system]]
		+	[[Category: P329g lala]]

OCA: Protected "6s5a" [edit=sysop:move=sysop]

OCA — Wed, 10 Jul 2019 04:25:04 GMT

Protected "6s5a" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 10 July 2019

OCA: New page: '''Unreleased structure''' The entry 6s5a is ON HOLD Authors: Ehler, A., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., Birk, M., Freimoser-Grundschober, A., Bruenker, ...

OCA — Wed, 10 Jul 2019 04:25:03 GMT

New page: '''Unreleased structure''' The entry 6s5a is ON HOLD Authors: Ehler, A., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., Birk, M., Freimoser-Grundschober, A., Bruenker, ...

New page

'''Unreleased structure'''

The entry 6s5a is ON HOLD

Authors: Ehler, A., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., Birk, M., Freimoser-Grundschober, A., Bruenker, P., Moessner, E., Umana, P., Kobold, S., Klein, C.

Description: CRYSTAL STRUCTURE OF FC P329G LALA WITH ANTI FC P329G FAB
[[Category: Unreleased Structures]]
[[Category: Kobold, S]]
[[Category: Ehler, A]]
[[Category: Wessels, U]]
[[Category: Klein, C]]
[[Category: Jost, C]]
[[Category: Moessner, E]]
[[Category: Umana, P]]
[[Category: Birk, M]]
[[Category: Freimoser-Grundschober, A]]
[[Category: Stubenrauch, K]]
[[Category: Benz, J]]
[[Category: Darowski, D]]
[[Category: Bruenker, P]]

6s5a - Revision history

OCA at 10:30, 26 February 2020

OCA: Protected "6s5a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6s5a is ON HOLD Authors: Ehler, A., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., Birk, M., Freimoser-Grundschober, A., Bruenker, ...