6s9i - Revision history

OCA at 10:31, 26 February 2020

OCA — Wed, 26 Feb 2020 10:31:21 GMT

←Older revision		Revision as of 10:31, 26 February 2020
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	<StructureSection load='6s9i' size='340' side='right'caption='[[6s9i]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='6s9i' size='340' side='right'caption='[[6s9i]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s9i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s9i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9I FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SNRNP200, ASCC3L1, HELIC2, KIAA0788 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PRPF8, PRPC8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9i OCA], [http://pdbe.org/6s9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9i RCSB], [http://www.ebi.ac.uk/pdbsum/6s9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9i OCA], [http://pdbe.org/6s9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9i RCSB], [http://www.ebi.ac.uk/pdbsum/6s9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9i ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]

OCA at 16:17, 22 January 2020

OCA — Wed, 22 Jan 2020 16:17:26 GMT

←Older revision		Revision as of 16:17, 22 January 2020
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-	'''Unreleased structure'''

-	The entry ~~6s9i~~ is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Human Brr2 Helicase Region D534C/N1866C in complex with C-tail deleted Jab1==
		+	<StructureSection load='6s9i' size='340' side='right'caption='[[6s9i]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s9i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9I FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9i OCA], [http://pdbe.org/6s9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9i RCSB], [http://www.ebi.ac.uk/pdbsum/6s9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9i ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/U520_HUMAN U520_HUMAN]] Retinitis pigmentosa. Retinitis pigmentosa 33 (RP33) [MIM:[http://omim.org/entry/610359 610359]]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Note=The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:16723661</ref> <ref>PMID:23045696</ref> <ref>PMID:19878916</ref> <ref>PMID:19710410</ref> <ref>PMID:21618346</ref> [[http://www.uniprot.org/uniprot/PRP8_HUMAN PRP8_HUMAN]] Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:[http://omim.org/entry/600059 600059]]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.<ref>PMID:17317632</ref> <ref>PMID:11468273</ref> [:]<ref>PMID:11910553</ref> <ref>PMID:12714658</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/U520_HUMAN U520_HUMAN]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:16723661</ref> <ref>PMID:8670905</ref> <ref>PMID:9539711</ref> <ref>PMID:23045696</ref> [[http://www.uniprot.org/uniprot/PRP8_HUMAN PRP8_HUMAN]] Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RNA helicase bad response to refrigeration 2 homolog (BRR2) is required for the activation of the spliceosome before the first catalytic step of RNA splicing. BRR2 represents a distinct subgroup of Ski2-like nucleic acid helicases whose members comprise tandem helicase cassettes. Only the N-terminal cassette of BRR2 is an active ATPase and can unwind substrate RNAs. The C-terminal cassette represents a pseudo-enzyme that can stimulate RNA-related activities of the N-terminal cassette. However, the molecular mechanisms by which the C-terminal cassette modulates the activities of the N-terminal unit remain elusive. Here, we show that N- and C-terminal cassettes adopt vastly different relative orientations in a crystal structure of BRR2 in complex with an activating domain of the spliceosomal Prp8 protein at 2.4 A resolution compared with the crystal structure of BRR2 alone. Likewise, inspection of BRR2 structures within spliceosomal complexes revealed that the cassettes occupy different relative positions and engage in different inter-cassette contacts during different splicing stages. Engineered disulfide bridges that locked the cassettes in two different relative orientations had opposite effects on the RNA unwinding activity of the N-terminal cassette, with one configuration enhancing and the other configuration inhibiting RNA unwinding compared with the unconstrained protein. Moreover, we found that differences in relative positioning of the cassettes strongly influence RNA-stimulated ATP hydrolysis by the N-terminal cassette. Our results indicate that the inactive C-terminal cassette of BRR2 can both positively and negatively affect the activity of the N-terminal helicase unit from a distance.

-	~~Authors~~:	+	The inactive C-terminal cassette of the dual-cassette RNA helicase BRR2 both stimulates and inhibits the activity of the N-terminal helicase unit.,Vester K, Santos KF, Kuropka B, Weise C, Wahl MC J Biol Chem. 2019 Dec 30. pii: RA119.010964. doi: 10.1074/jbc.RA119.010964. PMID:31914407<ref>PMID:31914407</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6s9i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: RNA helicase]]
		+	[[Category: Santos, K F]]
		+	[[Category: Vester, K]]
		+	[[Category: Wahl, M C]]
		+	[[Category: Dexd/h-box rna helicase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nucleus]]
		+	[[Category: Pre-mrna splicing]]
		+	[[Category: Rna and atp binding]]
		+	[[Category: Rnp remodeling]]
		+	[[Category: Spliceosome catalytic activation]]

OCA: Protected "6s9i" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2019 04:41:21 GMT

Protected "6s9i" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:41, 24 July 2019

OCA: New page: '''Unreleased structure''' The entry 6s9i is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 Jul 2019 04:41:20 GMT

New page: '''Unreleased structure''' The entry 6s9i is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6s9i is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]