6s9s - Revision history

OCA at 08:11, 23 October 2019

OCA — Wed, 23 Oct 2019 08:11:50 GMT

←Older revision		Revision as of 08:11, 23 October 2019
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	<StructureSection load='6s9s' size='340' side='right'caption='[[6s9s]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6s9s' size='340' side='right'caption='[[6s9s]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s9s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9s OCA], [http://pdbe.org/6s9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9s RCSB], [http://www.ebi.ac.uk/pdbsum/6s9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9s ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ldb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9s OCA], [http://pdbe.org/6s9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9s RCSB], [http://www.ebi.ac.uk/pdbsum/6s9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: African clawed frog]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Bienz, M]]		[[Category: Bienz, M]]
	[[Category: Pluckthun, A]]		[[Category: Pluckthun, A]]

OCA at 05:26, 10 October 2019

OCA — Thu, 10 Oct 2019 05:26:56 GMT

←Older revision		Revision as of 05:26, 10 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6s9s is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Dimerization domain of Xenopus laevis LDB1 in complex with darpin 10==
		+	<StructureSection load='6s9s' size='340' side='right'caption='[[6s9s]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s9s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9S FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9s OCA], [http://pdbe.org/6s9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9s RCSB], [http://www.ebi.ac.uk/pdbsum/6s9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9s ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LDB1_XENLA LDB1_XENLA]] Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. Acts as a coactivator together with otx2 to stimulate lhx1/lim1-mediated activation of the gsc promoter in the Spemann organizer. Acts synergistically with lhx1/lim1 and ssbp in axis formation.<ref>PMID:10926781</ref> <ref>PMID:12381786</ref> <ref>PMID:8918878</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Chip/LIM-domain binding protein (LDB)-single-stranded DNA-binding protein (SSDP) (ChiLS) complex controls numerous cell-fate decisions in animal cells, by mediating transcription of developmental control genes via remote enhancers. ChiLS is recruited to these enhancers by lineage-specific LIM-domain proteins that bind to its Chip/LDB subunit. ChiLS recently emerged as the core module of the Wnt enhanceosome, a multiprotein complex that primes developmental control genes for timely Wnt responses. ChiLS binds to NPFxD motifs within Pygopus (Pygo) and the Osa/ARID1A subunit of the BAF chromatin remodeling complex, which could synergize with LIM proteins in tethering ChiLS to enhancers. Chip/LDB and SSDP both contain N-terminal dimerization domains that constitute the bulk of their structured cores. Here, we report the crystal structures of these dimerization domains, in part aided by DARPin chaperones. We conducted systematic surface scanning by structure-designed mutations, followed by in vitro and in vivo binding assays, to determine conserved surface residues required for binding between Chip/LDB, SSDP, and Pygo-NPFxD. Based on this, and on the 4:2 (SSDP-Chip/LDB) stoichiometry of ChiLS, we derive a highly constrained structural model for this complex, which adopts a rotationally symmetrical SSDP2-LDB2-SSDP2 architecture. Integrity of ChiLS is essential for Pygo binding, and our mutational analysis places the NPFxD pockets on either side of the Chip/LDB dimer, each flanked by an SSDP dimer. The symmetry and multivalency of ChiLS underpin its function as an enhancer module integrating Wnt signals with lineage-specific factors to operate context-dependent transcriptional switches that are pivotal for normal development and cancer.

-	~~Authors~~:	+	Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome.,Renko M, Fiedler M, Rutherford TJ, Schaefer JV, Pluckthun A, Bienz M Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1912705116. doi:, 10.1073/pnas.1912705116. PMID:31570581<ref>PMID:31570581</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6s9s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bienz, M]]
		+	[[Category: Pluckthun, A]]
		+	[[Category: Renko, M]]
		+	[[Category: Schaefer, J V]]
		+	[[Category: Gene regulation]]
		+	[[Category: Wnt enhanceosome]]
		+	[[Category: Wnt signalling]]

OCA at 04:27, 7 August 2019

OCA — Wed, 07 Aug 2019 04:27:08 GMT

←Older revision		Revision as of 04:27, 7 August 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6s9s is ON HOLD	+	The entry 6s9s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6s9s" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2019 04:42:06 GMT

Protected "6s9s" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:42, 24 July 2019

OCA: New page: '''Unreleased structure''' The entry 6s9s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 Jul 2019 04:42:05 GMT

New page: '''Unreleased structure''' The entry 6s9s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6s9s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]