6sao - Revision history

OCA at 09:43, 30 October 2019

OCA — Wed, 30 Oct 2019 09:43:07 GMT

←Older revision		Revision as of 09:43, 30 October 2019
Line 3:		Line 3:
	<StructureSection load='6sao' size='340' side='right'caption='[[6sao]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='6sao' size='340' side='right'caption='[[6sao]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6sao]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sao]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thamnidium_elegans Thamnidium elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SAO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sao OCA], [http://pdbe.org/6sao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sao RCSB], [http://www.ebi.ac.uk/pdbsum/6sao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sao ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sao OCA], [http://pdbe.org/6sao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sao RCSB], [http://www.ebi.ac.uk/pdbsum/6sao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sao ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amylases are probably the best studied glycoside hydrolases and have a huge biotechnological value for industrial processes on starch. Multiple amylases from fungi and microbes are currently in use. Whereas bacterial amylases are well suited for many industrial processes due to their high stability, fungal amylases are recognized as safe and are preferred in the food industry, although they lack the pH tolerance and stability of their bacterial counterparts. Here, we describe three amylases, two of which have a broad pH spectrum extending to pH 8 and higher stability well suited for a broad set of industrial applications. These enzymes have the characteristic GH13 alpha-amylase fold with a central (beta/alpha)8-domain, an insertion domain with the canonical calcium binding site and a C-terminal beta-sandwich domain. The active site was identified based on the binding of the inhibitor acarbose in form of a transglycosylation product, in the amylases from Thamnidium elegans and Cordyceps farinosa. The three amylases have shortened loops flanking the nonreducing end of the substrate binding cleft, creating a more open crevice. Moreover, a potential novel binding site in the C-terminal domain of the Cordyceps enzyme was identified, which might be part of a starch interaction site. In addition, Cordyceps farinosa amylase presented a successful example of using the microseed matrix screening technique to significantly speed-up crystallization.
		+
		+	Structural and Functional Characterization of Three Novel Fungal Amylases with Enhanced Stability and pH Tolerance.,Roth C, Moroz OV, Turkenburg JP, Blagova E, Waterman J, Ariza A, Ming L, Tianqi S, Andersen C, Davies GJ, Wilson KS Int J Mol Sci. 2019 Oct 3;20(19). pii: ijms20194902. doi: 10.3390/ijms20194902. PMID:31623309<ref>PMID:31623309</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sao" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Thamnidium elegans]]
	[[Category: Andersen, C]]		[[Category: Andersen, C]]
	[[Category: Ariza, A]]		[[Category: Ariza, A]]

OCA at 07:03, 23 October 2019

OCA — Wed, 23 Oct 2019 07:03:05 GMT

←Older revision		Revision as of 07:03, 23 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6sao ~~is ON HOLD~~	+	==Structural and functional characterisation of three novel fungal amylases with enhanced stability and pH tolerance==
-		+	<StructureSection load='6sao' size='340' side='right'caption='[[6sao]], [[Resolution\|resolution]] 1.20Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6sao]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SAO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sao OCA], [http://pdbe.org/6sao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sao RCSB], [http://www.ebi.ac.uk/pdbsum/6sao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sao ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Andersen, C]]
		+	[[Category: Ariza, A]]
		+	[[Category: Blagova, E]]
		+	[[Category: Davies, G J]]
		+	[[Category: Ming, L]]
		+	[[Category: Moroz, O V]]
		+	[[Category: Roth, C]]
		+	[[Category: Tianqi, S]]
		+	[[Category: Turkenburg, J P]]
		+	[[Category: Waterman, J]]
		+	[[Category: Wilson, K S]]
		+	[[Category: Amylase]]
		+	[[Category: Biotechnology]]
		+	[[Category: Complex]]
		+	[[Category: Fungal]]
		+	[[Category: Hydrolase]]

OCA: Protected "6sao" [edit=sysop:move=sysop]

OCA — Wed, 24 Jul 2019 04:45:10 GMT

Protected "6sao" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:45, 24 July 2019

OCA: New page: '''Unreleased structure''' The entry 6sao is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 Jul 2019 04:45:08 GMT

New page: '''Unreleased structure''' The entry 6sao is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6sao is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]