6sbf - Revision history

OCA at 12:38, 24 January 2024

OCA — Wed, 24 Jan 2024 12:38:48 GMT

←Older revision		Revision as of 12:38, 24 January 2024
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	<StructureSection load='6sbf' size='340' side='right'caption='[[6sbf]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='6sbf' size='340' side='right'caption='[[6sbf]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6sbf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Scytonema_sp.~~_pcc_10023~~ Scytonema sp. ~~pcc~~ 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6SBF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_sp._PCC_10023 Scytonema sp. PCC 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SBF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BME:BETA~~-~~MERCAPTOETHANOL'>BME</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6sbb\|6sbb]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">mstE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680591 Scytonema sp. PCC 10023])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbf OCA], [https://pdbe.org/6sbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sbf RCSB], [https://www.ebi.ac.uk/pdbsum/6sbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6sbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbf OCA], [~~http~~://pdbe.org/6sbf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6sbf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6sbf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2D1CM82_9CYAN A0A2D1CM82_9CYAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Scytonema sp. ~~pcc~~ 10023]]	+	[[Category: Scytonema sp. PCC 10023]]
-	[[Category: Cahn~~, J K.B~~]]	+	[[Category: Cahn JKB]]
-	[[Category: Ecker, F]]	+	[[Category: Ecker F]]
-	[[Category: Groll, M]]	+	[[Category: Groll M]]
-	[[Category: Leopold-Messer, S]]	+	[[Category: Leopold-Messer S]]
-	[[Category: Moosmann, P]]	+	[[Category: Moosmann P]]
-	[[Category: Piel, J]]	+	[[Category: Piel J]]
-	~~[[Category: Alpha6-alpha6 barrel]]~~	+
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Marine drug]]~~	+
-	~~[[Category: Merosterol]]~~	+
-	~~[[Category: Type ii terpene cyclase~~]]	+

OCA at 06:12, 14 October 2020

OCA — Wed, 14 Oct 2020 06:12:45 GMT

←Older revision		Revision as of 06:12, 14 October 2020
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	==Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)==		==Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)==
-	<StructureSection load='6sbf' size='340' side='right'caption='[[6sbf]]' scene=''>	+	<StructureSection load='6sbf' size='340' side='right'caption='[[6sbf]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scytonema_sp._pcc_10023 Scytonema sp. pcc 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbf OCA], [http://pdbe.org/6sbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbf RCSB], [http://www.ebi.ac.uk/pdbsum/6sbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbf ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6sbb\|6sbb]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mstE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680591 Scytonema sp. PCC 10023])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbf OCA], [http://pdbe.org/6sbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbf RCSB], [http://www.ebi.ac.uk/pdbsum/6sbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Class II terpene cyclases, such as oxidosqualene and squalene-hopene cyclases, catalyse some of the most complex polycyclization reactions. They minimally exhibit a beta,gamma-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the corresponding class II counterparts have not been previously reported. Here we present high-resolution X-ray structures of a monodomain class II cyclase, merosterolic acid synthase (MstE). With a minimalistic beta-domain architecture, this cyanobacterial enzyme is able to construct four rings in cytotoxic meroterpenoids with a sterol-like topology. The structures with bound substrate, product, and inhibitor provide detailed snapshots of a cyclization mechanism largely governed by residues located in a noncanonical enzyme region. Our results complement the few known class II cyclase crystal structures, while also indicating that archaic monodomain cyclases might have already catalyzed complex reaction cascades.
		+
		+	A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.,Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689<ref>PMID:32778689</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sbf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cahn ~~JKB~~]]	+	[[Category: Scytonema sp. pcc 10023]]
-	[[Category: Ecker F]]	+	[[Category: Cahn, J K.B]]
-	[[Category: Groll M]]	+	[[Category: Ecker, F]]
-	[[Category: Leopold-Messer S]]	+	[[Category: Groll, M]]
-	[[Category: Moosmann P]]	+	[[Category: Leopold-Messer, S]]
-	[[Category: Piel J]]	+	[[Category: Moosmann, P]]
		+	[[Category: Piel, J]]
		+	[[Category: Alpha6-alpha6 barrel]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Marine drug]]
		+	[[Category: Merosterol]]
		+	[[Category: Type ii terpene cyclase]]

OCA at 06:33, 19 August 2020

OCA — Wed, 19 Aug 2020 06:33:27 GMT

←Older revision		Revision as of 06:33, 19 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6sbf ~~is ON HOLD until Paper Publication~~	+	==Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)==
-		+	<StructureSection load='6sbf' size='340' side='right'caption='[[6sbf]]' scene=''>
-	~~Authors~~: ~~Moosmann, P~~.~~, Ecker, F~~., ~~Leopold~~-~~Messer~~, S., ~~Cahn, J~~.K.B., ~~Groll, M~~., ~~Piel, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBF FirstGlance]. <br>
-	~~Description: Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbf OCA], [http://pdbe.org/6sbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbf RCSB], [http://www.ebi.ac.uk/pdbsum/6sbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Moosmann, P~~]]	+	__TOC__
-	[[Category: ~~Piel, J~~]]	+	</StructureSection>
-	[[Category: ~~Cahn, J.K.B~~]]	+	[[Category: Large Structures]]
-	[[Category: Leopold-Messer, S]]	+	[[Category: Cahn JKB]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Ecker F]]
-	[[Category: ~~Ecker, F~~]]	+	[[Category: Groll M]]
		+	[[Category: Leopold-Messer S]]
		+	[[Category: Moosmann P]]
		+	[[Category: Piel J]]

OCA: Protected "6sbf" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:54:58 GMT

Protected "6sbf" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6sbf is ON HOLD until Paper Publication Authors: Moosmann, P., Ecker, F., Leopold-Messer, S., Cahn, J.K.B., Groll, M., Piel, J. Description: Struct...

OCA — Wed, 31 Jul 2019 04:54:56 GMT

New page: '''Unreleased structure''' The entry 6sbf is ON HOLD until Paper Publication Authors: Moosmann, P., Ecker, F., Leopold-Messer, S., Cahn, J.K.B., Groll, M., Piel, J. Description: Struct...

New page

'''Unreleased structure'''

The entry 6sbf is ON HOLD until Paper Publication

Authors: Moosmann, P., Ecker, F., Leopold-Messer, S., Cahn, J.K.B., Groll, M., Piel, J.

Description: Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)
[[Category: Unreleased Structures]]
[[Category: Moosmann, P]]
[[Category: Piel, J]]
[[Category: Cahn, J.K.B]]
[[Category: Leopold-Messer, S]]
[[Category: Groll, M]]
[[Category: Ecker, F]]