OCA at 12:40, 24 January 2024

OCA — Wed, 24 Jan 2024 12:40:30 GMT

←Older revision		Revision as of 12:40, 24 January 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6se9 is ~~ON HOLD~~	+	==Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with lactose bound in shallow mode==
		+	<StructureSection load='6se9' size='340' side='right'caption='[[6se9]], [[Resolution\|resolution]] 1.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6se9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_sp._32cB Arthrobacter sp. 32cB]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SE9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.965Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6se9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6se9 OCA], [https://pdbe.org/6se9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6se9 RCSB], [https://www.ebi.ac.uk/pdbsum/6se9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6se9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A023UGN9_9MICC A0A023UGN9_9MICC]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ArthbetaDG is a dimeric, cold-adapted beta-d-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthbetaDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthbetaDG_E441Q mutein is an inactive form of the enzyme designed to enable observation of enzyme interaction with its substrate. The resulting three-dimensional structures of complexes: ArthbetaDG_E441Q/LACs and ArthbetaDG/IPTG (ligand bound in shallow mode) and structures of complexes ArthbetaDG_E441Q/LACd, ArthbetaDG/ONPG (ligands bound in deep mode), and galactose ArthbetaDG/GAL and their analysis enabled structural characterization of the hydrolysis reaction mechanism. Furthermore, comparative analysis with mesophilic analogs revealed the most striking differences in catalysis mechanisms. The key role in substrate transfer from shallow to deep binding mode involves rotation of the F581 side chain. It is worth noting that the 10-aa loop restricting access to the active site in mesophilic GH2 betaDGs, in ArthbetaDG is moved outward. This facilitates access of substrate to active site. Such a permanent exposure of the entrance to the active site may be a key factor for improved turnover rate of the cold adapted enzyme and thus a structural feature related to its cold adaptation.

-	~~Authors: Rutkiewicz~~, M., Bujacz, A., ~~Bujacz~~, G.	+	Active Site Architecture and Reaction Mechanism Determination of Cold Adapted beta-d-galactosidase from Arthrobacter sp. 32cB.,Rutkiewicz M, Bujacz A, Wanarska M, Wierzbicka-Wos A, Cieslinski H Int J Mol Sci. 2019 Sep 3;20(17). pii: ijms20174301. doi: 10.3390/ijms20174301. PMID:31484304<ref>PMID:31484304</ref>

-	~~Description: Cold~~-~~adapted beta~~-~~D-galactosidase from~~ Arthrobacter sp. 32cB ~~mutant E441Q in complex with lactose bound in shallow mode~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Rutkiewicz, M~~]]	+	<div class="pdbe-citations 6se9" style="background-color:#fffaf0;"></div>
-	[[Category: Bujacz~~, A~~]]	+
-	[[Category: ~~Bujacz, G~~]]	+	==See Also==
		+	*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arthrobacter sp. 32cB]]
		+	[[Category: Large Structures]]
		+	[[Category: Bujacz A]]
		+	[[Category: Bujacz G]]
		+	[[Category: Rutkiewicz M]]

OCA: Protected "6se9" [edit=sysop:move=sysop]

OCA — Wed, 07 Aug 2019 04:31:35 GMT

Protected "6se9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:31, 7 August 2019

OCA: New page: '''Unreleased structure''' The entry 6se9 is ON HOLD Authors: Rutkiewicz, M., Bujacz, A., Bujacz, G. Description: Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E4...

OCA — Wed, 07 Aug 2019 04:31:34 GMT

New page: '''Unreleased structure''' The entry 6se9 is ON HOLD Authors: Rutkiewicz, M., Bujacz, A., Bujacz, G. Description: Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E4...

New page

'''Unreleased structure'''

The entry 6se9 is ON HOLD

Authors: Rutkiewicz, M., Bujacz, A., Bujacz, G.

Description: Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with lactose bound in shallow mode
[[Category: Unreleased Structures]]
[[Category: Rutkiewicz, M]]
[[Category: Bujacz, A]]
[[Category: Bujacz, G]]

6se9 - Revision history

OCA at 12:40, 24 January 2024

OCA: Protected "6se9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6se9 is ON HOLD Authors: Rutkiewicz, M., Bujacz, A., Bujacz, G. Description: Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E4...