6sf0 - Revision history

OCA at 12:40, 24 January 2024

OCA — Wed, 24 Jan 2024 12:40:59 GMT

←Older revision		Revision as of 12:40, 24 January 2024
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	<StructureSection load='6sf0' size='340' side='right'caption='[[6sf0]], [[Resolution\|resolution]] 3.01Å' scene=''>		<StructureSection load='6sf0' size='340' side='right'caption='[[6sf0]], [[Resolution\|resolution]] 3.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6sf0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6SF0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sf0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SF0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.01Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6sf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf0 OCA], [~~http~~://pdbe.org/6sf0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6sf0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6sf0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf0 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf0 OCA], [https://pdbe.org/6sf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sf0 RCSB], [https://www.ebi.ac.uk/pdbsum/6sf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6sf0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6sf0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Bailleul, G]]	+	[[Category: Bailleul G]]
-	[[Category: Fiorentini, F]]	+	[[Category: Fiorentini F]]
-	[[Category: Fraaije, M]]	+	[[Category: Fraaije M]]
-	[[Category: Mascotti~~, M L~~]]	+	[[Category: Mascotti ML]]
-	[[Category: Mattevi, A]]	+	[[Category: Mattevi A]]
-	[[Category: Nicoll, C]]	+	[[Category: Nicoll C]]
-	~~[[Category: Ancestral sequence reconstruction]]~~	+
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Flavin]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 12:16, 1 January 2020

OCA — Wed, 01 Jan 2020 12:16:01 GMT

←Older revision		Revision as of 12:16, 1 January 2020
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	<StructureSection load='6sf0' size='340' side='right'caption='[[6sf0]], [[Resolution\|resolution]] 3.01Å' scene=''>		<StructureSection load='6sf0' size='340' side='right'caption='[[6sf0]], [[Resolution\|resolution]] 3.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6sf0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SF0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sf0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SF0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf0 OCA], [http://pdbe.org/6sf0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sf0 RCSB], [http://www.ebi.ac.uk/pdbsum/6sf0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf0 OCA], [http://pdbe.org/6sf0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sf0 RCSB], [http://www.ebi.ac.uk/pdbsum/6sf0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavin-containing monooxygenases (FMOs) are ubiquitous in all domains of life and metabolize a myriad of xenobiotics, including toxins, pesticides and drugs. However, despite their pharmacological importance, structural information remains bereft. To further our understanding behind their biochemistry and diversity, we used ancestral-sequence reconstruction, kinetic and crystallographic techniques to scrutinize three ancient mammalian FMOs: AncFMO2, AncFMO3-6 and AncFMO5. Remarkably, all AncFMOs could be crystallized and were structurally resolved between 2.7- and 3.2-A resolution. These crystal structures depict the unprecedented topology of mammalian FMOs. Each employs extensive membrane-binding features and intricate substrate-profiling tunnel networks through a conspicuous membrane-adhering insertion. Furthermore, a glutamate-histidine switch is speculated to induce the distinctive Baeyer-Villiger oxidation activity of FMO5. The AncFMOs exhibited catalysis akin to human FMOs and, with sequence identities between 82% and 92%, represent excellent models. Our study demonstrates the power of ancestral-sequence reconstruction as a strategy for the crystallization of proteins.
		+
		+	Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.,Nicoll CR, Bailleul G, Fiorentini F, Mascotti ML, Fraaije MW, Mattevi A Nat Struct Mol Biol. 2019 Dec 23. pii: 10.1038/s41594-019-0347-2. doi:, 10.1038/s41594-019-0347-2. PMID:31873300<ref>PMID:31873300</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sf0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Bailleul, G]]		[[Category: Bailleul, G]]
	[[Category: Fiorentini, F]]		[[Category: Fiorentini, F]]

OCA at 09:17, 25 December 2019

OCA — Wed, 25 Dec 2019 09:17:57 GMT

←Older revision		Revision as of 09:17, 25 December 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6sf0 ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 2 in the presence of NADP+==
-		+	<StructureSection load='6sf0' size='340' side='right'caption='[[6sf0]], [[Resolution\|resolution]] 3.01Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6sf0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SF0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf0 OCA], [http://pdbe.org/6sf0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sf0 RCSB], [http://www.ebi.ac.uk/pdbsum/6sf0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf0 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bailleul, G]]
		+	[[Category: Fiorentini, F]]
		+	[[Category: Fraaije, M]]
		+	[[Category: Mascotti, M L]]
		+	[[Category: Mattevi, A]]
		+	[[Category: Nicoll, C]]
		+	[[Category: Ancestral sequence reconstruction]]
		+	[[Category: Enzyme]]
		+	[[Category: Flavin]]
		+	[[Category: Membrane protein]]
		+	[[Category: Oxidoreductase]]

OCA at 15:09, 14 August 2019

OCA — Wed, 14 Aug 2019 15:09:21 GMT

←Older revision		Revision as of 15:09, 14 August 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6sf0 is ON HOLD	+	The entry 6sf0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6sf0" [edit=sysop:move=sysop]

OCA — Wed, 07 Aug 2019 04:33:16 GMT

Protected "6sf0" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:33, 7 August 2019

OCA: New page: '''Unreleased structure''' The entry 6sf0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Aug 2019 04:33:15 GMT

New page: '''Unreleased structure''' The entry 6sf0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6sf0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]