6sff - Revision history

OCA at 12:41, 24 January 2024

OCA — Wed, 24 Jan 2024 12:41:14 GMT

←Older revision		Revision as of 12:41, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6sff' size='340' side='right'caption='[[6sff]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6sff' size='340' side='right'caption='[[6sff]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6sff]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6SFF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sff]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SFF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Il12b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sff OCA], [~~http~~://pdbe.org/6sff PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6sff RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6sff PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6sff ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sff OCA], [https://pdbe.org/6sff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sff RCSB], [https://www.ebi.ac.uk/pdbsum/6sff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sff ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IL12B_MOUSE IL12B_MOUSE]] Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.<ref>PMID:11114383</ref>	+	[https://www.uniprot.org/uniprot/IL12B_MOUSE IL12B_MOUSE] Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.<ref>PMID:11114383</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Bloch, Y]]	+	[[Category: Bloch Y]]
-	[[Category: Savvides~~, S N]]~~	+	[[Category: Savvides SN]]
-	~~[[Category: Antagonist]]~~	+
-	~~[[Category: Cytokine]]~~	+
-	~~[[Category: Fibronectin]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Secreted glycoprotein~~]]	+

OCA at 06:55, 30 December 2020

OCA — Wed, 30 Dec 2020 06:55:41 GMT

←Older revision		Revision as of 06:55, 30 December 2020
Line 1:		Line 1:

	==mouse Interleukin-12 subunit beta - p80 homodimer in space group I41==		==mouse Interleukin-12 subunit beta - p80 homodimer in space group I41==
-	<StructureSection load='6sff' size='340' side='right'caption='[[6sff]]' scene=''>	+	<StructureSection load='6sff' size='340' side='right'caption='[[6sff]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SFF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sff]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SFF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sff OCA], [http://pdbe.org/6sff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sff RCSB], [http://www.ebi.ac.uk/pdbsum/6sff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sff ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Il12b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sff OCA], [http://pdbe.org/6sff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sff RCSB], [http://www.ebi.ac.uk/pdbsum/6sff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sff ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IL12B_MOUSE IL12B_MOUSE]] Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.<ref>PMID:11114383</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structural studies of glycoproteins and their complexes provide critical insights into their roles in normal physiology and disease. Most glycoproteins contain N-linked glycosylation, a key post-translation modification that critically affects protein folding and stability and the binding kinetics underlying protein interactions. However, N-linked glycosylation is often an impediment to yielding homogeneous protein preparations for structure determination by X-ray crystallography or other methods. In particular, obtaining diffraction-quality crystals of such proteins and their complexes often requires modification of both the type of glycosylation patterns and their extent. Here, we demonstrate the benefits of producing target glycoproteins in the GlycoDelete human embryonic kidney 293 cell line that has been engineered to produce N-glycans as short glycan stumps comprising N-acetylglucosamine, galactose and sialic acid. Protein fragments of human Down syndrome cell-adhesion molecule and colony-stimulating factor 1 receptor were obtained from the GlycoDelete cell line for crystallization. The ensuing reduction in the extent and complexity of N-glycosylation in both protein molecules compared with alternative glycoengineering approaches enabled their productive deployment in structural studies by X-ray crystallography. Furthermore, a third successful implementation of the GlycoDelete technology focusing on murine IL-12B is shown to lead to N-glycosylation featuring an immature glycan in diffraction-quality crystals. It is proposed that the GlycoDelete cell line could serve as a valuable go-to option for the production of homogeneous glycoproteins and their complexes for structural studies by X-ray crystallography and cryo-electron microscopy.
		+
		+	Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis.,Kozak S, Bloch Y, De Munck S, Mikula A, Bento I, Savvides SN, Meijers R Acta Crystallogr D Struct Biol. 2020 Dec 1;76(Pt 12):1244-1255. doi:, 10.1107/S2059798320013753. Epub 2020 Nov 24. PMID:33263330<ref>PMID:33263330</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sff" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Interleukin 3D structures\|Interleukin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bloch Y]]	+	[[Category: Lk3 transgenic mice]]
-	[[Category: Savvides SN]]	+	[[Category: Bloch, Y]]
		+	[[Category: Savvides, S N]]
		+	[[Category: Antagonist]]
		+	[[Category: Cytokine]]
		+	[[Category: Fibronectin]]
		+	[[Category: Homodimer]]
		+	[[Category: Secreted glycoprotein]]

OCA at 11:13, 26 August 2020

OCA — Wed, 26 Aug 2020 11:13:50 GMT

←Older revision		Revision as of 11:13, 26 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6sff is ON HOLD until Paper Publication~~	+	==mouse Interleukin-12 subunit beta - p80 homodimer in space group I41==
-		+	<StructureSection load='6sff' size='340' side='right'caption='[[6sff]]' scene=''>
-	~~Authors: Bloch, Y., Savvides, S.N.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SFF FirstGlance]. <br>
-	~~Description:~~ mouse Interleukin-12 subunit beta -p80 homodimer in space group I41	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sff OCA], [http://pdbe.org/6sff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sff RCSB], [http://www.ebi.ac.uk/pdbsum/6sff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sff ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Savvides~~, S.N]]	+	__TOC__
-	[[Category: Bloch, Y]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bloch Y]]
		+	[[Category: Savvides SN]]

OCA at 05:54, 22 July 2020

OCA — Wed, 22 Jul 2020 05:54:00 GMT

←Older revision		Revision as of 05:54, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6sff is ON HOLD	+	The entry 6sff is ON HOLD until Paper Publication

	Authors: Bloch, Y., Savvides, S.N.		Authors: Bloch, Y., Savvides, S.N.

OCA: Protected "6sff" [edit=sysop:move=sysop]

OCA — Wed, 07 Aug 2019 04:34:17 GMT

Protected "6sff" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 7 August 2019

OCA: New page: '''Unreleased structure''' The entry 6sff is ON HOLD Authors: Bloch, Y., Savvides, S.N. Description: mouse Interleukin-12 subunit beta -p80 homodimer in space group I41 [[Category: Unr...

OCA — Wed, 07 Aug 2019 04:34:15 GMT

New page: '''Unreleased structure''' The entry 6sff is ON HOLD Authors: Bloch, Y., Savvides, S.N. Description: mouse Interleukin-12 subunit beta -p80 homodimer in space group I41 [[Category: Unr...

New page

'''Unreleased structure'''

The entry 6sff is ON HOLD

Authors: Bloch, Y., Savvides, S.N.

Description: mouse Interleukin-12 subunit beta -p80 homodimer in space group I41
[[Category: Unreleased Structures]]
[[Category: Savvides, S.N]]
[[Category: Bloch, Y]]