OCA at 10:00, 12 August 2020

OCA — Wed, 12 Aug 2020 10:00:19 GMT

←Older revision		Revision as of 10:00, 12 August 2020
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	==Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2, in the presence of ssDNA==		==Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2, in the presence of ssDNA==
-	<StructureSection load='6sh8' size='340' side='right'caption='[[6sh8]]' scene=''>	+	<StructureSection load='6sh8' size='340' side='right'caption='[[6sh8]], [[Resolution\|resolution]] 3.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SH8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SH8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sh8]] is a 39 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Sulir Sulir] and [http://en.wikipedia.org/wiki/Sulfolobus_islandicus_rey15a Sulfolobus islandicus rey15a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SH8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SH8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sh8 OCA], [http://pdbe.org/6sh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sh8 RCSB], [http://www.ebi.ac.uk/pdbsum/6sh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sh8 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6s91\|6s91]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SiRe_0602 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=930945 SULIR]), SiRe_0599 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=930945 SULIR])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sh8 OCA], [http://pdbe.org/6sh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sh8 RCSB], [http://www.ebi.ac.uk/pdbsum/6sh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sh8 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cmr-beta is a type III-B CRISPR-Cas complex that, upon target RNA recognition, unleashes a multifaceted immune response against invading genetic elements, including single-stranded DNA (ssDNA) cleavage, cyclic oligoadenylate synthesis, and also a unique UA-specific single-stranded RNA (ssRNA) hydrolysis by the Cmr2 subunit. Here, we present the structure-function relationship of Cmr-beta, unveiling how binding of the target RNA regulates the Cmr2 activities. Cryoelectron microscopy (cryo-EM) analysis revealed the unique subunit architecture of Cmr-beta and captured the complex in different conformational stages of the immune response, including the non-cognate and cognate target-RNA-bound complexes. The binding of the target RNA induces a conformational change of Cmr2, which together with the complementation between the 5' tag in the CRISPR RNAs (crRNA) and the 3' antitag of the target RNA activate different configurations in a unique loop of the Cmr3 subunit, which acts as an allosteric sensor signaling the self- versus non-self-recognition. These findings highlight the diverse defense strategies of type III complexes.
		+
		+	Structures of the Cmr-beta Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas.,Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G Mol Cell. 2020 Jul 29. pii: S1097-2765(20)30474-3. doi:, 10.1016/j.molcel.2020.07.008. PMID:32730741<ref>PMID:32730741</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sh8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Montoya G]]	+	[[Category: Sulfolobus islandicus rey15a]]
-	[[Category: Sofos N]]	+	[[Category: Sulir]]
-	[[Category: Stella S]]	+	[[Category: Montoya, G]]
		+	[[Category: Sofos, N]]
		+	[[Category: Stella, S]]
		+	[[Category: Antiviral protein]]
		+	[[Category: Crispr-ca]]
		+	[[Category: Cyclic oligo-adenylate synthase]]
		+	[[Category: Effector complex]]
		+	[[Category: Nuclease]]

OCA at 14:04, 8 July 2020

OCA — Wed, 08 Jul 2020 14:04:41 GMT

←Older revision		Revision as of 14:04, 8 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6sh8 is ~~ON HOLD~~	+	==Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2, in the presence of ssDNA==
-		+	<StructureSection load='6sh8' size='340' side='right'caption='[[6sh8]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SH8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SH8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sh8 OCA], [http://pdbe.org/6sh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sh8 RCSB], [http://www.ebi.ac.uk/pdbsum/6sh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sh8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Montoya G]]
		+	[[Category: Sofos N]]
		+	[[Category: Stella S]]

OCA: Protected "6sh8" [edit=sysop:move=sysop]

OCA — Wed, 14 Aug 2019 15:13:28 GMT

Protected "6sh8" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:13, 14 August 2019

OCA: New page: '''Unreleased structure''' The entry 6sh8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Aug 2019 15:13:27 GMT

New page: '''Unreleased structure''' The entry 6sh8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6sh8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6sh8 - Revision history

OCA at 10:00, 12 August 2020

OCA at 14:04, 8 July 2020

OCA: Protected "6sh8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6sh8 is ON HOLD Authors: Description: Category: Unreleased Structures