OCA at 09:26, 27 November 2019

OCA — Wed, 27 Nov 2019 09:26:12 GMT

←Older revision		Revision as of 09:26, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6sj8 is ~~ON HOLD~~	+	==Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofolate==
		+	<StructureSection load='6sj8' size='340' side='right'caption='[[6sj8]], [[Resolution\|resolution]] 1.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6sj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Deshd Deshd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SJ8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=44V:(6S)-2-AMINO-6-METHYL-5,6,7,8-TETRAHYDROPTERIDIN-4(3H)-ONE'>44V</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dhaf_4327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272564 DESHD])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tetrahydromethanopterin_S-methyltransferase Tetrahydromethanopterin S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.86 2.1.1.86] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sj8 OCA], [http://pdbe.org/6sj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6sj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sj8 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes orchestrating methylation between tetrahydrofolate (THF) and cobalamin (Cbl) are abundant among all domains of life. During energy production in Desulfitobacterium hafniense, MtgA catalyzes the methyl transfer from methylcobalamin (Cbl-CH3 ) to THF in the catabolism of glycine betaine (GB). Despite its lack of sequence identity with known structures, we could show that MtgA forms a homodimeric complex of two TIM barrels. Atomic crystallographic insights into the interplay of MtgA with THF as well as analysis of a trapped reaction intermediate (THF-CH3 )(+) reveal conformational rearrangements during the transfer reaction. Whereas residues for THF methylation are conserved, the binding mode for the THF glutamyl-p-aminobenzoate moiety (THF tail) is unique. Apart from snapshots of individual reaction steps of MtgA, structure-based mutagenesis combined with enzymatic activity assays allowed a mechanistic description of the methyl transfer between Cbl-CH3 and THF. Altogether, the THF-tail-binding motion observed in MtgA is unique compared to other THF methyltransferases and therefore contributes to the general understanding of THF-mediated methyl transfer.

-	~~Authors: Badmann~~, T., Groll, M.	+	Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution.,Badmann T, Groll M Chembiochem. 2019 Sep 13. doi: 10.1002/cbic.201900515. PMID:31518049<ref>PMID:31518049</ref>

-	~~Description~~: ~~Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofolate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6sj8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Deshd]]
		+	[[Category: Large Structures]]
		+	[[Category: Tetrahydromethanopterin S-methyltransferase]]
	[[Category: Badmann, T]]		[[Category: Badmann, T]]
	[[Category: Groll, M]]		[[Category: Groll, M]]
		+	[[Category: Anaerobic bacteria]]
		+	[[Category: Cobalamin]]
		+	[[Category: Glycine betaine metabolism]]
		+	[[Category: Methyl transfer]]
		+	[[Category: Tetrahydrofolate]]
		+	[[Category: Transferase]]

OCA: Protected "6sj8" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2019 04:18:49 GMT

Protected "6sj8" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:18, 21 August 2019

OCA: New page: '''Unreleased structure''' The entry 6sj8 is ON HOLD Authors: Badmann, T., Groll, M. Description: Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofol...

OCA — Wed, 21 Aug 2019 04:18:48 GMT

New page: '''Unreleased structure''' The entry 6sj8 is ON HOLD Authors: Badmann, T., Groll, M. Description: Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofol...

New page

'''Unreleased structure'''

The entry 6sj8 is ON HOLD

Authors: Badmann, T., Groll, M.

Description: Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofolate
[[Category: Unreleased Structures]]
[[Category: Badmann, T]]
[[Category: Groll, M]]

6sj8 - Revision history

OCA at 09:26, 27 November 2019

OCA: Protected "6sj8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6sj8 is ON HOLD Authors: Badmann, T., Groll, M. Description: Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofol...