OCA at 11:28, 22 July 2020

OCA — Wed, 22 Jul 2020 11:28:35 GMT

←Older revision		Revision as of 11:28, 22 July 2020
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	==Crystal structure of the Legionella pneumophila type II secretion system substrate NttE==		==Crystal structure of the Legionella pneumophila type II secretion system substrate NttE==
-	<StructureSection load='6skw' size='340' side='right'caption='[[6skw]]' scene=''>	+	<StructureSection load='6skw' size='340' side='right'caption='[[6skw]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6skw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_130b Legionella pneumophila 130b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SKW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skw OCA], [http://pdbe.org/6skw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6skw RCSB], [http://www.ebi.ac.uk/pdbsum/6skw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6skw ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6sjt\|6sjt]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D7214_10670, DIZ48_11715, DIZ50_11270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=866628 Legionella pneumophila 130b])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skw OCA], [http://pdbe.org/6skw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6skw RCSB], [http://www.ebi.ac.uk/pdbsum/6skw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6skw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Legionella pneumophila is a Gram-negative bacterium that is able to replicate within a broad range of aquatic protozoan hosts. L. pneumophila is also an opportunistic human pathogen that can infect macrophages and epithelia in the lung and lead to Legionnaires' disease. The type II secretion system is a key virulence factor of L. pneumophila and is used to promote bacterial growth at low temperatures, regulate biofilm formation, modulate host responses to infection, facilitate bacterial penetration of mucin gels and is necessary for intracellular growth during the initial stages of infection. The L. pneumophila type II secretion system exports at least 25 substrates out of the bacterium and several of these, including NttA to NttG, contain unique amino acid sequences that are generally not observed outside of the Legionella genus. NttA, NttC, and NttD are required for infection of several amoebal species but it is unclear what influence other novel substrates have within their host. In this study, we show that NttE is required for optimal infection of Acanthamoeba castellanii and Vermamoeba vermiformis amoeba and is essential for the typical colony morphology of L. pneumophila. In addition, we report the atomic structures of NttA, NttC, and NttE and through a combined biophysical and biochemical hypothesis driven approach we propose novel functions for these substrates during infection. This work lays the foundation for future studies into the mechanistic understanding of novel type II substrate functions and how these relate to L. pneumophila ecology and disease.
		+
		+	Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System.,Portlock TJ, Tyson JY, Dantu SC, Rehman S, White RC, McIntire IE, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto NP, Garnett JA Front Mol Biosci. 2020 Jun 11;7:112. doi: 10.3389/fmolb.2020.00112. eCollection, 2020. PMID:32656228<ref>PMID:32656228</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6skw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Garnett JA]]	+	[[Category: Legionella pneumophila 130b]]
-	[[Category: Portlock TJ]]	+	[[Category: Garnett, J A]]
-	[[Category: Rehman S]]	+	[[Category: Portlock, T J]]
		+	[[Category: Rehman, S]]
		+	[[Category: Legionella pneumophila]]
		+	[[Category: Type ii secretion system]]
		+	[[Category: Unknown function]]

OCA at 05:47, 20 May 2020

OCA — Wed, 20 May 2020 05:47:22 GMT

←Older revision		Revision as of 05:47, 20 May 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6skw is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the Legionella pneumophila type II secretion system substrate NttE==
-		+	<StructureSection load='6skw' size='340' side='right'caption='[[6skw]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SKW FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skw OCA], [http://pdbe.org/6skw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6skw RCSB], [http://www.ebi.ac.uk/pdbsum/6skw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6skw ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Garnett JA]]
		+	[[Category: Portlock TJ]]
		+	[[Category: Rehman S]]

OCA: Protected "6skw" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 13:51:54 GMT

Protected "6skw" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:51, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6skw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Aug 2019 13:51:52 GMT

New page: '''Unreleased structure''' The entry 6skw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6skw is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6skw - Revision history

OCA at 11:28, 22 July 2020

OCA at 05:47, 20 May 2020

OCA: Protected "6skw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6skw is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures