6skx - Revision history

OCA at 12:43, 24 January 2024

OCA — Wed, 24 Jan 2024 12:43:24 GMT

←Older revision		Revision as of 12:43, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6skx' size='340' side='right'caption='[[6skx]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='6skx' size='340' side='right'caption='[[6skx]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6skx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aspfu Aspfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6skx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SKX FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>AFUA_5G01250 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=330879 ASPFU])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3~~-~~hydroxyisobutyrate_dehydrogenase 3-hydroxyisobutyrate dehydrogenase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.31 1.1.1.31] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6skx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skx OCA], [https://pdbe.org/6skx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6skx RCSB], [https://www.ebi.ac.uk/pdbsum/6skx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6skx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6skx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skx OCA], [https://pdbe.org/6skx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6skx RCSB], [https://www.ebi.ac.uk/pdbsum/6skx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6skx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4WDZ8_ASPFU Q4WDZ8_ASPFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~3-hydroxyisobutyrate dehydrogenase]]~~	+	[[Category: Aspergillus fumigatus Af293]]
-	~~[[Category: Aspfu~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grogan, G]]	+	[[Category: Grogan G]]
-	[[Category: Mangas-Sanchez, J]]	+	[[Category: Mangas-Sanchez J]]
-	[[Category: Sharma, M]]	+	[[Category: Sharma M]]
-	[[Category: Turner~~, N J]]~~	+	[[Category: Turner NJ]]
-	~~[[Category: Amine]]~~	+
-	~~[[Category: Imine]]~~	+
-	~~[[Category: Nadph]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:06, 7 July 2021

OCA — Wed, 07 Jul 2021 10:06:59 GMT

←Older revision		Revision as of 10:06, 7 July 2021
Line 1:		Line 1:

	==Structure of Reductive Aminase from Neosartorya fumigata==		==Structure of Reductive Aminase from Neosartorya fumigata==
-	<StructureSection load='6skx' size='340' side='right'caption='[[6skx]]' scene=''>	+	<StructureSection load='6skx' size='340' side='right'caption='[[6skx]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6SKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6skx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SKX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6skx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skx OCA], [~~http~~://pdbe.org/6skx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6skx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6skx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6skx ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AFUA_5G01250 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-hydroxyisobutyrate_dehydrogenase 3-hydroxyisobutyrate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.31 1.1.1.31] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6skx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skx OCA], [https://pdbe.org/6skx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6skx RCSB], [https://www.ebi.ac.uk/pdbsum/6skx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6skx ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chiral primary amines are important intermediates in the synthesis of pharmaceutical compounds. Fungal reductive aminases (RedAms) are NADPH-dependent dehydrogenases that catalyse reductive amination of a range of ketones with short-chain primary amines supplied in an equimolar ratio to give corresponding secondary amines. Herein we describe structural and biochemical characterisation as well as synthetic applications of two RedAms from Neosartorya spp. (NfRedAm and NfisRedAm) that display a distinctive activity amongst fungal RedAms, namely a superior ability to use ammonia as the amine partner. Using these enzymes, we demonstrate the synthesis of a broad range of primary amines, with conversions up to >97% and excellent enantiomeric excess. Temperature dependent studies showed that these homologues also possess greater thermal stability compared to other enzymes within this family. Their synthetic applicability is further demonstrated by the production of several primary and secondary amines with turnover numbers (TN) up to 14 000 as well as continous flow reactions, obtaining chiral amines such as (R)-2-aminohexane in space time yields up to 8.1 g L(-1) h(-1). The remarkable features of NfRedAm and NfisRedAm highlight their potential for wider synthetic application as well as expanding the biocatalytic toolbox available for chiral amine synthesis.
		+
		+	Asymmetric synthesis of primary amines catalyzed by thermotolerant fungal reductive aminases.,Mangas-Sanchez J, Sharma M, Cosgrove SC, Ramsden JI, Marshall JR, Thorpe TW, Palmer RB, Grogan G, Turner NJ Chem Sci. 2020 May 5;11(19):5052-5057. doi: 10.1039/d0sc02253e. PMID:34122962<ref>PMID:34122962</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6skx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: 3-hydroxyisobutyrate dehydrogenase]]
		+	[[Category: Aspfu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grogan G]]	+	[[Category: Grogan, G]]
-	[[Category: Mangas-Sanchez J]]	+	[[Category: Mangas-Sanchez, J]]
-	[[Category: Sharma M]]	+	[[Category: Sharma, M]]
-	[[Category: Turner NJ]]	+	[[Category: Turner, N J]]
		+	[[Category: Amine]]
		+	[[Category: Imine]]
		+	[[Category: Nadph]]
		+	[[Category: Oxidoreductase]]

OCA at 06:20, 25 June 2020

OCA — Thu, 25 Jun 2020 06:20:57 GMT

←Older revision		Revision as of 06:20, 25 June 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6skx ~~is ON HOLD until Paper Publication~~	+	==Structure of Reductive Aminase from Neosartorya fumigata==
-		+	<StructureSection load='6skx' size='340' side='right'caption='[[6skx]]' scene=''>
-	~~Authors~~: ~~Sharma, M~~., ~~Mangas~~-~~Sanchez~~, J., ~~Turner~~, N.J., ~~Grogan, G~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SKX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SKX FirstGlance]. <br>
-	~~Description: Structure of Reductive Aminase from Neosartorya fumigata~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6skx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6skx OCA], [http://pdbe.org/6skx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6skx RCSB], [http://www.ebi.ac.uk/pdbsum/6skx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6skx ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Grogan, G]]	+	__TOC__
-	[[Category: ~~Sharma, M~~]]	+	</StructureSection>
-	[[Category: ~~Mangas-Sanchez, J~~]]	+	[[Category: Large Structures]]
-	[[Category: Turner~~, N.J~~]]	+	[[Category: Grogan G]]
		+	[[Category: Mangas-Sanchez J]]
		+	[[Category: Sharma M]]
		+	[[Category: Turner NJ]]

OCA: Protected "6skx" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 13:51:58 GMT

Protected "6skx" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:51, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6skx is ON HOLD until Paper Publication Authors: Sharma, M., Mangas-Sanchez, J., Turner, N.J., Grogan, G. Description: Structure of Reductive Amina...

OCA — Wed, 28 Aug 2019 13:51:56 GMT

New page: '''Unreleased structure''' The entry 6skx is ON HOLD until Paper Publication Authors: Sharma, M., Mangas-Sanchez, J., Turner, N.J., Grogan, G. Description: Structure of Reductive Amina...

New page

'''Unreleased structure'''

The entry 6skx is ON HOLD until Paper Publication

Authors: Sharma, M., Mangas-Sanchez, J., Turner, N.J., Grogan, G.

Description: Structure of Reductive Aminase from Neosartorya fumigata
[[Category: Unreleased Structures]]
[[Category: Grogan, G]]
[[Category: Sharma, M]]
[[Category: Mangas-Sanchez, J]]
[[Category: Turner, N.J]]