OCA at 11:29, 22 July 2020

OCA — Wed, 22 Jul 2020 11:29:23 GMT

←Older revision		Revision as of 11:29, 22 July 2020
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	==Cryo-electron microscopy structure of a RbcL-Raf1 supercomplex from Synechococcus elongatus PCC 7942==		==Cryo-electron microscopy structure of a RbcL-Raf1 supercomplex from Synechococcus elongatus PCC 7942==
-	<StructureSection load='6smh' size='340' side='right'caption='[[6smh]]' scene=''>	+	<StructureSection load='6smh' size='340' side='right'caption='[[6smh]], [[Resolution\|resolution]] 4.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SMH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6smh]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Anacystis_nidulans_r2 Anacystis nidulans r2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SMH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6smh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smh OCA], [http://pdbe.org/6smh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6smh RCSB], [http://www.ebi.ac.uk/pdbsum/6smh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6smh ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cbbL, rbcL, Synpcc7942_1426 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1140 Anacystis nidulans R2]), Synpcc7942_0833 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1140 Anacystis nidulans R2])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6smh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smh OCA], [http://pdbe.org/6smh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6smh RCSB], [http://www.ebi.ac.uk/pdbsum/6smh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6smh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RBL_SYNE7 RBL_SYNE7]] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carboxysomes are membrane-free organelles for carbon assimilation in cyanobacteria. The carboxysome consists of a proteinaceous shell that structurally resembles virus capsids and internal enzymes including ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), the primary carbon-fixing enzyme in photosynthesis. The formation of carboxysomes requires hierarchical self-assembly of thousands of protein subunits, initiated from Rubisco assembly and packaging to shell encapsulation. Here we study the role of Rubisco assembly factor 1 (Raf1) in Rubisco assembly and carboxysome formation in a model cyanobacterium, Synechococcus elongatus PCC7942 (Syn7942). Cryo-electron microscopy reveals that Raf1 facilitates Rubisco assembly by mediating RbcL dimer formation and dimer-dimer interactions. Syn7942 cells lacking Raf1 are unable to form canonical intact carboxysomes but generate a large number of intermediate assemblies comprising Rubisco, CcaA, CcmM, and CcmN without shell encapsulation and a low abundance of carboxysome-like structures with reduced dimensions and irregular shell shapes and internal organization. As a consequence, the Raf1-depleted cells exhibit reduced Rubisco content, CO2-fixing activity, and cell growth. Our results provide mechanistic insight into the chaperone-assisted Rubisco assembly and biogenesis of carboxysomes. Advanced understanding of the biogenesis and stepwise formation process of the biogeochemically important organelle may inform strategies for heterologous engineering of functional CO2-fixing modules to improve photosynthesis.
		+
		+	Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis.,Huang F, Kong WW, Sun Y, Chen T, Dykes GF, Jiang YL, Liu LN Proc Natl Acad Sci U S A. 2020 Jul 7. pii: 2007990117. doi:, 10.1073/pnas.2007990117. PMID:32636267<ref>PMID:32636267</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6smh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Anacystis nidulans r2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen T]]	+	[[Category: Ribulose-bisphosphate carboxylase]]
-	[[Category: Dykes GF]]	+	[[Category: Chen, T]]
-	[[Category: Huang F]]	+	[[Category: Dykes, G F]]
-	[[Category: Jiang YL]]	+	[[Category: Huang, F]]
-	[[Category: Kong W-W]]	+	[[Category: Jiang, Y L]]
-	[[Category: Liu LN]]	+	[[Category: Kong, W W]]
-	[[Category: Sun Y]]	+	[[Category: Liu, L N]]
		+	[[Category: Sun, Y]]
		+	[[Category: Cyanobacteria]]
		+	[[Category: Photosynthesis]]
		+	[[Category: Raf1]]
		+	[[Category: Rubisco]]
		+	[[Category: Rubisco accumulation factor1]]
		+	[[Category: Synechococcus elongatus 7942]]

OCA at 14:04, 8 July 2020

OCA — Wed, 08 Jul 2020 14:04:59 GMT

←Older revision		Revision as of 14:04, 8 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6smh is ~~ON HOLD~~	+	==Cryo-electron microscopy structure of a RbcL-Raf1 supercomplex from Synechococcus elongatus PCC 7942==
-		+	<StructureSection load='6smh' size='340' side='right'caption='[[6smh]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SMH FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6smh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smh OCA], [http://pdbe.org/6smh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6smh RCSB], [http://www.ebi.ac.uk/pdbsum/6smh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6smh ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen T]]
		+	[[Category: Dykes GF]]
		+	[[Category: Huang F]]
		+	[[Category: Jiang YL]]
		+	[[Category: Kong W-W]]
		+	[[Category: Liu LN]]
		+	[[Category: Sun Y]]

OCA: Protected "6smh" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 13:58:01 GMT

Protected "6smh" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:58, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6smh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Aug 2019 13:58:00 GMT

New page: '''Unreleased structure''' The entry 6smh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6smh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6smh - Revision history

OCA at 11:29, 22 July 2020

OCA at 14:04, 8 July 2020

OCA: Protected "6smh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6smh is ON HOLD Authors: Description: Category: Unreleased Structures