6st1 - Revision history

OCA at 12:47, 24 January 2024

OCA — Wed, 24 Jan 2024 12:47:29 GMT

←Older revision		Revision as of 12:47, 24 January 2024
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	<StructureSection load='6st1' size='340' side='right'caption='[[6st1]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='6st1' size='340' side='right'caption='[[6st1]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6st1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ST1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6st1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ST1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6st1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st1 OCA], [~~http~~://pdbe.org/6st1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6st1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6st1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6st1 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6st1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st1 OCA], [https://pdbe.org/6st1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6st1 RCSB], [https://www.ebi.ac.uk/pdbsum/6st1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6st1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TAUA_ECOLI TAUA_ECOLI]] Part of a binding-protein-dependent transport system for taurine.	+	[https://www.uniprot.org/uniprot/TAUA_ECOLI TAUA_ECOLI] Part of a binding-protein-dependent transport system for taurine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beis, K]]	+	[[Category: Beis K]]
-	[[Category: Qu, F]]	+	[[Category: Qu F]]
-	~~[[Category: Abc transporter]]~~	+
-	~~[[Category: Taurine substrate binding protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:55, 18 December 2019

OCA — Wed, 18 Dec 2019 07:55:27 GMT

←Older revision		Revision as of 07:55, 18 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6st1 is ~~ON HOLD until Paper~~ Publication	+	==Taurine ABC transporter substrate binding protein TauA from E. coli in complex with 2-(N-Morpholino)ethanesulfonic acid (MES)==
		+	<StructureSection load='6st1' size='340' side='right'caption='[[6st1]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6st1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ST1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6st1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st1 OCA], [http://pdbe.org/6st1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6st1 RCSB], [http://www.ebi.ac.uk/pdbsum/6st1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6st1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TAUA_ECOLI TAUA_ECOLI]] Part of a binding-protein-dependent transport system for taurine.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Under limiting sulfur availability, bacteria can assimilate sulfur from alkanesulfonates. Bacteria utilize ATP-binding cassette (ABC) transporters to internalise them for further processing to release sulfur. In gram-negative bacteria the TauABC and SsuABC ensure internalization, although, these two systems have common substrates, the former has been characterized as a taurine specific system. TauA and SsuA are substrate-binding proteins (SBPs) that bind and bring the alkanesulfonates to the ABC importer for transport. Here, we have determined the crystal structure of TauA and have characterized its thermodynamic binding parameters by isothermal titration calorimetry in complex with taurine and different alkanesulfonates. Our structures revealed that the coordination of the alkanesulfonates is conserved, with the exception of Asp205 that is absent from SsuA, but the thermodynamic parameters revealed a very high enthalpic penalty cost for binding of the other alkanesulfonates relative to taurine. Our molecular dynamic simulations indicated that the different levels of hydration of the binding site contributed to the selectivity for taurine over the other alkanesulfonates. Such selectivity mechanism is very likely to be employed by other SBPs of ABC transporters.

-	~~Authors: Beis, K~~., Qu, F.	+	Desolvation of the substrate-binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC.,Qu F, ElOmari K, Wagner A, De Simone A, Beis K Biochem J. 2019 Dec 12;476(23):3649-3660. doi: 10.1042/BCJ20190779. PMID:31802112<ref>PMID:31802112</ref>

-	~~Description: Taurine ABC transporter substrate binding protein TauA from E~~. ~~coli in complex with 2~~-(N-~~Morpholino)ethanesulfonic acid (MES)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Qu, F~~]]	+	<div class="pdbe-citations 6st1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Beis, K]]		[[Category: Beis, K]]
		+	[[Category: Qu, F]]
		+	[[Category: Abc transporter]]
		+	[[Category: Taurine substrate binding protein]]
		+	[[Category: Transport protein]]

OCA: Protected "6st1" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:43:27 GMT

Protected "6st1" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6st1 is ON HOLD until Paper Publication Authors: Beis, K., Qu, F. Description: Taurine ABC transporter substrate binding protein TauA from E. coli ...

OCA — Wed, 02 Oct 2019 07:43:26 GMT

New page: '''Unreleased structure''' The entry 6st1 is ON HOLD until Paper Publication Authors: Beis, K., Qu, F. Description: Taurine ABC transporter substrate binding protein TauA from E. coli ...

New page

'''Unreleased structure'''

The entry 6st1 is ON HOLD until Paper Publication

Authors: Beis, K., Qu, F.

Description: Taurine ABC transporter substrate binding protein TauA from E. coli in complex with 2-(N-Morpholino)ethanesulfonic acid (MES)
[[Category: Unreleased Structures]]
[[Category: Qu, F]]
[[Category: Beis, K]]