6sto - Revision history

OCA at 12:48, 24 January 2024

OCA — Wed, 24 Jan 2024 12:48:01 GMT

←Older revision		Revision as of 12:48, 24 January 2024
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	==Three dimensional structure of the giant reed (Arundodonax) lectin (ADL) complex with N-Acetyl lactosamine==		==Three dimensional structure of the giant reed (Arundodonax) lectin (ADL) complex with N-Acetyl lactosamine==
-	<StructureSection load='6sto' size='340' side='right'caption='[[6sto]]' scene=''>	+	<StructureSection load='6sto' size='340' side='right'caption='[[6sto]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6STO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6STO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sto]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arundo_donax Arundo donax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6STO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6STO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sto OCA], [https://pdbe.org/6sto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sto RCSB], [https://www.ebi.ac.uk/pdbsum/6sto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sto ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900019:N-acetyl-alpha-lactosamine'>PRD_900019</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sto OCA], [https://pdbe.org/6sto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sto RCSB], [https://www.ebi.ac.uk/pdbsum/6sto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sto ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0A9P802_ARUDO A0A0A9P802_ARUDO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Arundo donax lectin (ADL) is a 170 amino acid protein that can be purified from the rhizomes of the giant reed or giant cane exploiting its selective binding to chitin followed by elution with N-acetyl-D-glucosamine. The lectin is listed in the UniProt server, the largest protein sequence database, as an uncharacterized protein with chitin-binding domains (A0A0A9P802). This paper reports the purification, three-dimensional structure and ligand-binding properties of ADL. The lectin is a homodimer in which the two protomers are linked by two disulphide bridges. Each polypeptide chain presents four carbohydrate-binding modules that belong to family 18 (CBM18). A high degree of sequence similarity is observed among the modules present in each protomer. We have determined the X-ray structure of the apo-protein to a resolution of 1.70 A. The carbohydrate-binding modules, that span a sequence of approximately 40 amino acids, present four internal disulfide bridges a very short antiparallel central beta sheet and three short alpha helices, two on one side of the beta sheet and one on the other. The structures of the complexes of the lectin with N-acetylglucosamine, N-acetyllactosamine, N-acetylneuraminic acid and N-N'diacetylchitobiose reveal that ADL has two primary and two secondary carbohydrate-binding sites per dimer. They are located at the interface between the two protomers and each binding site involves residues of both chains. The lectin presents structural similarity to the wheat germ agglutinin (WGA) family, in particular to isoform 3.
		+
		+	Three-dimensional structure and properties of the giant reed (Arundo donax) lectin (ADL).,Perduca M, Bovi M, Destefanis L, Nadali D, Fin L, Parolini F, Sorio D, Carrizo ME, Monaco HL Glycobiology. 2021 Jun 29. pii: 6311232. doi: 10.1093/glycob/cwab059. PMID:34192315<ref>PMID:34192315</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sto" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arundo donax]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bovi M]]		[[Category: Bovi M]]

OCA at 09:48, 14 July 2021

OCA — Wed, 14 Jul 2021 09:48:44 GMT

←Older revision		Revision as of 09:48, 14 July 2021
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-	'''Unreleased structure'''

-	~~The entry 6sto is ON HOLD until Paper Publication~~	+	==Three dimensional structure of the giant reed (Arundodonax) lectin (ADL) complex with N-Acetyl lactosamine==
-		+	<StructureSection load='6sto' size='340' side='right'caption='[[6sto]]' scene=''>
-	~~Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6STO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6STO FirstGlance]. <br>
-	~~Description:~~ Three dimensional structure of the giant reed (Arundodonax) lectin (ADL) complex with N-Acetyl lactosamine	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sto OCA], [https://pdbe.org/6sto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sto RCSB], [https://www.ebi.ac.uk/pdbsum/6sto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sto ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Perduca,~~ M]]	+	__TOC__
-	[[Category: Carrizo~~, M.E~~]]	+	</StructureSection>
-	[[Category: ~~Nadali, D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Monaco, H.~~L]]	+	[[Category: Bovi M]]
-	[[Category: ~~Destefanis, L~~]]	+	[[Category: Carrizo ME]]
-	[[Category: ~~Bovi, M~~]]	+	[[Category: Destefanis L]]
-	[[Category: ~~Fin, L~~]]	+	[[Category: Fin L]]
		+	[[Category: Monaco HL]]
		+	[[Category: Nadali D]]
		+	[[Category: Perduca M]]

OCA at 05:59, 22 July 2020

OCA — Wed, 22 Jul 2020 05:59:34 GMT

←Older revision		Revision as of 05:59, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6sto is ON HOLD	+	The entry 6sto is ON HOLD until Paper Publication

	Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E.		Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E.
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	[[Category: Perduca, M]]		[[Category: Perduca, M]]
	[[Category: Carrizo, M.E]]		[[Category: Carrizo, M.E]]
-	[[Category: Monaco, H.L]]
-	[[Category: Bovi, M]]
	[[Category: Nadali, D]]		[[Category: Nadali, D]]
		+	[[Category: Monaco, H.L]]
	[[Category: Destefanis, L]]		[[Category: Destefanis, L]]
		+	[[Category: Bovi, M]]
	[[Category: Fin, L]]		[[Category: Fin, L]]

OCA: Protected "6sto" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:46:01 GMT

Protected "6sto" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6sto is ON HOLD Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E. Description: Three dimensional str...

OCA — Wed, 02 Oct 2019 07:45:59 GMT

New page: '''Unreleased structure''' The entry 6sto is ON HOLD Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E. Description: Three dimensional str...

New page

'''Unreleased structure'''

The entry 6sto is ON HOLD

Authors: Perduca, M., Monaco, H.L., Bovi, M., Destefanis, L., Nadali, D., Fin, L., Carrizo, M.E.

Description: Three dimensional structure of the giant reed (Arundodonax) lectin (ADL) complex with N-Acetyl lactosamine
[[Category: Unreleased Structures]]
[[Category: Perduca, M]]
[[Category: Carrizo, M.E]]
[[Category: Monaco, H.L]]
[[Category: Bovi, M]]
[[Category: Nadali, D]]
[[Category: Destefanis, L]]
[[Category: Fin, L]]