6su5 - Revision history

OCA at 06:52, 14 April 2021

OCA — Wed, 14 Apr 2021 06:52:15 GMT

←Older revision		Revision as of 06:52, 14 April 2021
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	==Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119==		==Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119==
-	<StructureSection load='6su5' size='340' side='right'caption='[[6su5]]' scene=''>	+	<StructureSection load='6su5' size='340' side='right'caption='[[6su5]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SU5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6SU5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6su5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_phage_2119 Thermus phage 2119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SU5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6su5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6su5 OCA], [~~http~~://pdbe.org/6su5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6su5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6su5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6su5 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6su5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6su5 OCA], [https://pdbe.org/6su5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6su5 RCSB], [https://www.ebi.ac.uk/pdbsum/6su5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6su5 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peptidoglycan hydrolytic enzymes are considered to be a promising alternative to conventional antibiotics in combating bacterial infections. To identify novel hydrolytic enzymes, we performed a database search with the sequences of two thermostable endolysins with high bactericidal activity, studied earlier in our laboratory. Both these enzymes originate from Thermus scotoductus bacteriophages MAT2119 and vB_Tsc2631. A lytic enzyme LysC from Clostridium intestinale URNW was found to have the highest amino acid sequence similarity to the bacteriophage proteins and was chosen for further analysis. The recombinant enzyme showed strong activity against its host bacteria C. intestinale, as well as against C. sporogenes, Bacillus cereus, Micrococcus luteus, and Staphylococcus aureus, on average causing a 5.12 +/- 0.14 log reduction of viable S. aureus ATCC 25923 cells in a bactericidal assay. Crystallographic studies of the protein showed that the catalytic site of LysC contained a zinc atom coordinated by amino acid residues His(50), His(147), and Cys(155), a feature characteristic for type 2 amidases. Surprisingly, neither of these residues, nor any other of the four conserved residues in the vicinity of the active site, His(51), Thr(52), Tyr(76), and Thr(153), were essential to maintain the antibacterial activity of LysC. Therefore, our attention was attracted to the intrinsically disordered and highly positively charged N-terminal region of the enzyme. Potential antibacterial activity of this part of the sequence, predicted by the Antimicrobial Sequence Scanning System, AMPA, was confirmed in our experimental studies; the truncated version of LysC (LysCDelta2-23) completely lacked antibacterial activity. Moreover, a synthetic peptide, which we termed Intestinalin, with a sequence identical to the first thirty amino acids of LysC, displayed substantial anti-staphylococcal activity with IC50 of 6 mug/mL (1.5 muM). This peptide was shown to have alpha-helical conformation in solution in the presence of detergents which is a common feature of amphipathic alpha-helical antimicrobial peptides.
		+
		+	Molecular Characterization of a Novel Lytic Enzyme LysC from Clostridium intestinale URNW and Its Antibacterial Activity Mediated by Positively Charged N-Terminal Extension.,Plotka M, Szadkowska M, Hakansson M, Kovacic R, Al-Karadaghi S, Walse B, Werbowy O, Kaczorowska AK, Kaczorowski T Int J Mol Sci. 2020 Jul 11;21(14). pii: ijms21144894. doi: 10.3390/ijms21144894. PMID:32664473<ref>PMID:32664473</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6su5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Al-Karadaghi S]]	+	[[Category: Thermus phage 2119]]
-	[[Category: Hakansson M]]	+	[[Category: Al-Karadaghi, S]]
-	[[Category: Kaczorowska AK]]	+	[[Category: Hakansson, M]]
-	[[Category: Kaczorowski T]]	+	[[Category: Kaczorowska, A K]]
-	[[Category: Plotka M]]	+	[[Category: Kaczorowski, T]]
		+	[[Category: Plotka, M]]
		+	[[Category: Amidase]]
		+	[[Category: Cell wall degradation]]
		+	[[Category: Endolysine]]
		+	[[Category: Hydrolase]]
		+	[[Category: Zinc binding]]

OCA at 07:06, 30 September 2020

OCA — Wed, 30 Sep 2020 07:06:58 GMT

←Older revision		Revision as of 07:06, 30 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6su5 ~~is ON HOLD until Paper Publication~~	+	==Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119==
-		+	<StructureSection load='6su5' size='340' side='right'caption='[[6su5]]' scene=''>
-	~~Authors~~: ~~Hakansson, M~~., Al-~~Karadaghi~~, S., ~~Plotka, M~~., ~~Kaczorowska, A~~.K., ~~Kaczorowski, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SU5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SU5 FirstGlance]. <br>
-	~~Description: Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6su5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6su5 OCA], [http://pdbe.org/6su5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6su5 RCSB], [http://www.ebi.ac.uk/pdbsum/6su5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6su5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Kaczorowska, A.K~~]]	+	__TOC__
-	[[Category: ~~Plotka,~~ M]]	+	</StructureSection>
-	[[Category: ~~Hakansson, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Al-Karadaghi, S~~]]	+	[[Category: Al-Karadaghi S]]
-	[[Category: ~~Kaczorowski, T~~]]	+	[[Category: Hakansson M]]
		+	[[Category: Kaczorowska AK]]
		+	[[Category: Kaczorowski T]]
		+	[[Category: Plotka M]]

OCA at 18:12, 24 June 2020

OCA — Wed, 24 Jun 2020 18:12:16 GMT

←Older revision		Revision as of 18:12, 24 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6su5 is ON HOLD	+	The entry 6su5 is ON HOLD until Paper Publication

	Authors: Hakansson, M., Al-Karadaghi, S., Plotka, M., Kaczorowska, A.K., Kaczorowski, T.		Authors: Hakansson, M., Al-Karadaghi, S., Plotka, M., Kaczorowska, A.K., Kaczorowski, T.
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	[[Category: Kaczorowska, A.K]]		[[Category: Kaczorowska, A.K]]
	[[Category: Plotka, M]]		[[Category: Plotka, M]]
-	[[Category: Kaczorowski, T]]
	[[Category: Hakansson, M]]		[[Category: Hakansson, M]]
	[[Category: Al-Karadaghi, S]]		[[Category: Al-Karadaghi, S]]
		+	[[Category: Kaczorowski, T]]

OCA: Protected "6su5" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:48:01 GMT

Protected "6su5" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:48, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6su5 is ON HOLD Authors: Hakansson, M., Al-Karadaghi, S., Plotka, M., Kaczorowska, A.K., Kaczorowski, T. Description: Ph2119 endolysin from Thermus...

OCA — Wed, 02 Oct 2019 07:47:59 GMT

New page: '''Unreleased structure''' The entry 6su5 is ON HOLD Authors: Hakansson, M., Al-Karadaghi, S., Plotka, M., Kaczorowska, A.K., Kaczorowski, T. Description: Ph2119 endolysin from Thermus...

New page

'''Unreleased structure'''

The entry 6su5 is ON HOLD

Authors: Hakansson, M., Al-Karadaghi, S., Plotka, M., Kaczorowska, A.K., Kaczorowski, T.

Description: Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119
[[Category: Unreleased Structures]]
[[Category: Kaczorowska, A.K]]
[[Category: Plotka, M]]
[[Category: Kaczorowski, T]]
[[Category: Hakansson, M]]
[[Category: Al-Karadaghi, S]]