6t2j - Revision history

OCA at 12:53, 24 January 2024

OCA — Wed, 24 Jan 2024 12:53:16 GMT

←Older revision		Revision as of 12:53, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6t2j' size='340' side='right'caption='[[6t2j]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6t2j' size='340' side='right'caption='[[6t2j]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6t2j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6T2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t2j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T2J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6t2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2j OCA], [~~http~~://pdbe.org/6t2j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6t2j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6t2j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2j ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2j OCA], [https://pdbe.org/6t2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t2j RCSB], [https://www.ebi.ac.uk/pdbsum/6t2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6t2j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6t2j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: House, D]]	+	[[Category: House D]]
-	[[Category: Rittinger, K]]	+	[[Category: Rittinger K]]
-	[[Category: Tsai, Y ~~C.I]]~~	+	[[Category: Tsai Y-CI]]
-	~~[[Category: Human single domain antibody]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 13:14, 18 December 2019

OCA — Wed, 18 Dec 2019 13:14:11 GMT

←Older revision		Revision as of 13:14, 18 December 2019
Line 3:		Line 3:
	<StructureSection load='6t2j' size='340' side='right'caption='[[6t2j]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6t2j' size='340' side='right'caption='[[6t2j]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6t2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6T2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t2j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6T2J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6t2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2j OCA], [http://pdbe.org/6t2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t2j RCSB], [http://www.ebi.ac.uk/pdbsum/6t2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6t2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2j OCA], [http://pdbe.org/6t2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t2j RCSB], [http://www.ebi.ac.uk/pdbsum/6t2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2j ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein ubiquitination plays a key role in the regulation of cellular processes, and misregulation of the ubiquitin system is linked to many diseases. So far, development of tool compounds that target enzymes of the ubiquitin system has been slow and only a few specific inhibitors are available. Here, we report the selection of single-domain antibodies (single-dAbs) based on a human scaffold that recognize the catalytic domain of HOIP, a subunit of the multi-component E3 LUBAC and member of the RBR family of E3 ligases. Some of these dAbs affect ligase activity and provide mechanistic insight into the ubiquitin transfer mechanism of different E2-conjugating enzymes. Furthermore, we show that the co-crystal structure of a HOIP RBR/dAb complex serves as a robust platform for soaking of ligands that target the active site cysteine of HOIP, thereby providing easy access to structure-based ligand design for this important class of E3 ligases.
		+
		+	Single-Domain Antibodies as Crystallization Chaperones to Enable Structure-Based Inhibitor Development for RBR E3 Ubiquitin Ligases.,Tsai YI, Johansson H, Dixon D, Martin S, Chung CW, Clarkson J, House D, Rittinger K Cell Chem Biol. 2019 Dec 2. pii: S2451-9456(19)30389-7. doi:, 10.1016/j.chembiol.2019.11.007. PMID:31813847<ref>PMID:31813847</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6t2j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: House, D]]		[[Category: House, D]]
	[[Category: Rittinger, K]]		[[Category: Rittinger, K]]

OCA at 06:59, 27 November 2019

OCA — Wed, 27 Nov 2019 06:59:46 GMT

←Older revision		Revision as of 06:59, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6t2j ~~is ON HOLD until Paper Publication~~	+	==dAb3==
-		+	<StructureSection load='6t2j' size='340' side='right'caption='[[6t2j]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6t2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6T2J FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6t2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2j OCA], [http://pdbe.org/6t2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t2j RCSB], [http://www.ebi.ac.uk/pdbsum/6t2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2j ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: House, D]]
		+	[[Category: Rittinger, K]]
		+	[[Category: Tsai, Y C.I]]
		+	[[Category: Human single domain antibody]]
		+	[[Category: Immune system]]

OCA: Protected "6t2j" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2019 04:28:45 GMT

Protected "6t2j" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:28, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6t2j is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Oct 2019 04:28:44 GMT

New page: '''Unreleased structure''' The entry 6t2j is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6t2j is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]