6t2l - Revision history

OCA at 12:53, 24 January 2024

OCA — Wed, 24 Jan 2024 12:53:16 GMT

←Older revision		Revision as of 12:53, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6t2l' size='340' side='right'caption='[[6t2l]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='6t2l' size='340' side='right'caption='[[6t2l]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6t2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T2L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T2L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HL9:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide'>HL9</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HL9:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide'>HL9</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2l OCA], [https://pdbe.org/6t2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t2l RCSB], [https://www.ebi.ac.uk/pdbsum/6t2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2l OCA], [https://pdbe.org/6t2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t2l RCSB], [https://www.ebi.ac.uk/pdbsum/6t2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hocker, B~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Lechner, H~~]]	+	[[Category: Hocker B]]
-	[[Category: ~~Artificial cofactor]]~~	+	[[Category: Lechner H]]
-	~~[[Category: Biotin-binding protein]]~~	+
-	~~[[Category: Catalyst]]~~	+
-	~~[[Category: Streptavidin~~]]	+

OCA at 05:27, 28 April 2021

OCA — Wed, 28 Apr 2021 05:27:25 GMT

←Older revision		Revision as of 05:27, 28 April 2021
Line 1:		Line 1:

	==Streptavidin variants harbouring an artificial organocatalyst based cofactor==		==Streptavidin variants harbouring an artificial organocatalyst based cofactor==
-	<StructureSection load='6t2l' size='340' side='right'caption='[[6t2l]]' scene=''>	+	<StructureSection load='6t2l' size='340' side='right'caption='[[6t2l]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6T2L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T2L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2l OCA], [~~http~~://pdbe.org/6t2l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6t2l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6t2l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2l ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HL9:5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide'>HL9</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2l OCA], [https://pdbe.org/6t2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t2l RCSB], [https://www.ebi.ac.uk/pdbsum/6t2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An artificial cofactor based on an organocatalyst embedded in a protein has been used to conduct the Baylis-Hillman reaction in a buffered system. As protein host, we chose streptavidin, as it can be easily crystallized and thereby supports the design process. The protein host around the cofactor was rationally designed on the basis of high-resolution crystal structures obtained after each variation of the amino acid sequence. Additionally, DFT-calculated intermediates and transition states were used to rationalize the observed activity. Finally, repeated cycles of structure determination and redesign led to a system with an up to one order of magnitude increase in activity over the bare cofactor and to the most active proteinogenic catalyst for the Baylis-Hillman reaction known today.
		+
		+	An Artificial Cofactor Catalyzing the Baylis-Hillman Reaction with Designed Streptavidin as Protein Host*.,Lechner H, Emann VR, Breuning M, Hocker B Chembiochem. 2021 Jan 5. doi: 10.1002/cbic.202000880. PMID:33400831<ref>PMID:33400831</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6t2l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hocker B]]	+	[[Category: Hocker, B]]
-	[[Category: Lechner H]]	+	[[Category: Lechner, H]]
		+	[[Category: Artificial cofactor]]
		+	[[Category: Biotin-binding protein]]
		+	[[Category: Catalyst]]
		+	[[Category: Streptavidin]]

OCA at 05:46, 14 October 2020

OCA — Wed, 14 Oct 2020 05:46:37 GMT

←Older revision		Revision as of 05:46, 14 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6t2l ~~is ON HOLD until Paper Publication~~	+	==Streptavidin variants harbouring an artificial organocatalyst based cofactor==
-		+	<StructureSection load='6t2l' size='340' side='right'caption='[[6t2l]]' scene=''>
-	~~Authors~~: ~~Lechner~~, H., ~~Hocker~~, B.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T2L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T2L FirstGlance]. <br>
-	~~Description: Streptavidin variants harbouring an artificial organocatalyst based cofactor~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t2l OCA], [http://pdbe.org/6t2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t2l RCSB], [http://www.ebi.ac.uk/pdbsum/6t2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t2l ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Lechner, H~~]]	+	__TOC__
-	[[Category: ~~Hocker, B~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hocker B]]
		+	[[Category: Lechner H]]

OCA: Protected "6t2l" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2019 04:28:52 GMT

Protected "6t2l" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:28, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6t2l is ON HOLD until Paper Publication Authors: Lechner, H., Hocker, B. Description: Streptavidin variants harbouring an artificial organocatalyst...

OCA — Wed, 16 Oct 2019 04:28:51 GMT

New page: '''Unreleased structure''' The entry 6t2l is ON HOLD until Paper Publication Authors: Lechner, H., Hocker, B. Description: Streptavidin variants harbouring an artificial organocatalyst...

New page

'''Unreleased structure'''

The entry 6t2l is ON HOLD until Paper Publication

Authors: Lechner, H., Hocker, B.

Description: Streptavidin variants harbouring an artificial organocatalyst based cofactor
[[Category: Unreleased Structures]]
[[Category: Lechner, H]]
[[Category: Hocker, B]]