6t9q - Revision history

OCA at 08:55, 18 February 2021

OCA — Thu, 18 Feb 2021 08:55:45 GMT

←Older revision		Revision as of 08:55, 18 February 2021
Line 3:		Line 3:
	<StructureSection load='6t9q' size='340' side='right'caption='[[6t9q]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='6t9q' size='340' side='right'caption='[[6t9q]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6t9q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T9Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6T9Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t9q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T9Q FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6taz\|6taz]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6taz\|6taz]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIMELESS, TIM, TIM1, TIMELESS1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIMELESS, TIM, TIM1, TIMELESS1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6t9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t9q OCA], [~~http~~://pdbe.org/6t9q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6t9q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6t9q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6t9q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t9q OCA], [https://pdbe.org/6t9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t9q RCSB], [https://www.ebi.ac.uk/pdbsum/6t9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t9q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN]] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>	+	[[https://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN]] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:46, 16 December 2020

OCA — Wed, 16 Dec 2020 12:46:31 GMT

←Older revision		Revision as of 12:46, 16 December 2020
Line 1:		Line 1:

	==Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless==		==Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless==
-	<StructureSection load='6t9q' size='340' side='right'caption='[[6t9q]]' scene=''>	+	<StructureSection load='6t9q' size='340' side='right'caption='[[6t9q]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T9Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T9Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6t9q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T9Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T9Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t9q OCA], [http://pdbe.org/6t9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t9q RCSB], [http://www.ebi.ac.uk/pdbsum/6t9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t9q ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6taz\|6taz]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIMELESS, TIM, TIM1, TIMELESS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t9q OCA], [http://pdbe.org/6t9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t9q RCSB], [http://www.ebi.ac.uk/pdbsum/6t9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t9q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN]] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Regions of the genome with the potential to form secondary DNA structures pose a frequent and significant impediment to DNA replication and must be actively managed in order to preserve genetic and epigenetic integrity. How the replisome detects and responds to secondary structures is poorly understood. Here, we show that a core component of the fork protection complex in the eukaryotic replisome, Timeless, harbours in its C-terminal region a previously unappreciated DNA-binding domain that exhibits specific binding to G-quadruplex (G4) DNA structures. We show that this domain contributes to maintaining processive replication through G4-forming sequences, and exhibits partial redundancy with an adjacent PARP-binding domain. Further, this function of Timeless requires interaction with and activity of the helicase DDX11. Loss of both Timeless and DDX11 causes epigenetic instability at G4-forming sequences and DNA damage. Our findings indicate that Timeless contributes to the ability of the replisome to sense replication-hindering G4 formation and ensures the prompt resolution of these structures by DDX11 to maintain processive DNA synthesis.
		+
		+	Timeless couples G-quadruplex detection with processing by DDX11 helicase during DNA replication.,Lerner LK, Holzer S, Kilkenny ML, Svikovic S, Murat P, Schiavone D, Eldridge CB, Bittleston A, Maman JD, Branzei D, Stott K, Pellegrini L, Sale JE EMBO J. 2020 Jul 23:e104185. doi: 10.15252/embj.2019104185. PMID:32705708<ref>PMID:32705708</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6t9q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Holzer S]]	+	[[Category: Holzer, S]]
-	[[Category: Pellegrini L]]	+	[[Category: Pellegrini, L]]
		+	[[Category: 3-helix bundle]]
		+	[[Category: Dna-binding]]
		+	[[Category: Replication]]
		+	[[Category: Tandem repeat]]

OCA at 10:00, 12 August 2020

OCA — Wed, 12 Aug 2020 10:00:39 GMT

←Older revision		Revision as of 10:00, 12 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6t9q is ON HOLD until Paper Publication~~	+	==Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless==
-		+	<StructureSection load='6t9q' size='340' side='right'caption='[[6t9q]]' scene=''>
-	~~Authors: Pellegrini, L., Holzer, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T9Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T9Q FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t9q OCA], [http://pdbe.org/6t9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t9q RCSB], [http://www.ebi.ac.uk/pdbsum/6t9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t9q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Holzer, S]]	+	__TOC__
-	[[Category: Pellegrini, L]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Holzer S]]
		+	[[Category: Pellegrini L]]

OCA: Protected "6t9q" [edit=sysop:move=sysop]

OCA — Wed, 06 Nov 2019 05:12:39 GMT

Protected "6t9q" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 6 November 2019

OCA: New page: '''Unreleased structure''' The entry 6t9q is ON HOLD until Paper Publication Authors: Pellegrini, L., Holzer, S. Description: Crystal structure of the second, C-terminal repeat of the ...

OCA — Wed, 06 Nov 2019 05:12:37 GMT

New page: '''Unreleased structure''' The entry 6t9q is ON HOLD until Paper Publication Authors: Pellegrini, L., Holzer, S. Description: Crystal structure of the second, C-terminal repeat of the ...

New page

'''Unreleased structure'''

The entry 6t9q is ON HOLD until Paper Publication

Authors: Pellegrini, L., Holzer, S.

Description: Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless
[[Category: Unreleased Structures]]
[[Category: Holzer, S]]
[[Category: Pellegrini, L]]