6tco - Revision history

OCA at 12:59, 24 January 2024

OCA — Wed, 24 Jan 2024 12:59:47 GMT

←Older revision		Revision as of 12:59, 24 January 2024
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	<StructureSection load='6tco' size='340' side='right'caption='[[6tco]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6tco' size='340' side='right'caption='[[6tco]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tco]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6TCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tco]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TCO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6tco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tco OCA], [~~http~~://pdbe.org/6tco PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tco RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tco PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tco ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tco OCA], [https://pdbe.org/6tco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tco RCSB], [https://www.ebi.ac.uk/pdbsum/6tco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tco ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6tco" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6tco" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beavil~~, A J~~]]	+	[[Category: Beavil AJ]]
-	[[Category: Ceska, T]]	+	[[Category: Ceska T]]
-	[[Category: Davies~~, A M~~]]	+	[[Category: Davies AM]]
-	[[Category: Henry~~, A J~~]]	+	[[Category: Henry AJ]]
-	[[Category: McDonnell~~, J M~~]]	+	[[Category: McDonnell JM]]
-	[[Category: Mitropoulou~~, A N~~]]	+	[[Category: Mitropoulou AN]]
-	[[Category: Sutton~~, B J]]~~	+	[[Category: Sutton BJ]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 09:53, 18 March 2020

OCA — Wed, 18 Mar 2020 09:53:41 GMT

←Older revision		Revision as of 09:53, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6tco is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the omalizumab Fab Leu158Pro light chain mutant - crystal form I==
		+	<StructureSection load='6tco' size='340' side='right'caption='[[6tco]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6tco]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TCO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tco OCA], [http://pdbe.org/6tco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tco RCSB], [http://www.ebi.ac.uk/pdbsum/6tco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tco ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Immunoglobulin E (IgE) plays a central role in the allergic response, in which cross-linking of allergen by FcepsilonRI-bound IgE triggers mast cell and basophil degranulation and the release of inflammatory mediators. The high-affinity interaction between IgE and FcepsilonRI is a long-standing target for therapeutic intervention in allergic disease. Omalizumab is a clinically approved anti-IgE monoclonal antibody that binds to free IgE, also with high affinity, preventing its interaction with FcepsilonRI. All attempts to crystallize the pre-formed complex between the omalizumab Fab and the Fc region of IgE (IgE-Fc), to understand the structural basis for its mechanism of action, surprisingly failed. Instead, the Fab alone selectively crystallized in different crystal forms, but their structures revealed intermolecular Fab/Fab interactions that were clearly strong enough to disrupt the Fab/IgE-Fc complexes. Some of these interactions were common to other Fab crystal structures. Mutations were therefore designed to disrupt two recurring packing interactions observed in the omalizumab Fab crystal structures without interfering with the ability of the omalizumab Fab to recognize IgE-Fc; this led to the successful crystallization and subsequent structure determination of the Fab/IgE-Fc complex. The mutagenesis strategy adopted to achieve this result is applicable to other intractable Fab/antigen complexes or systems in which Fabs are used as crystallization chaperones.

-	~~Authors~~:	+	Engineering the Fab fragment of the anti-IgE omalizumab to prevent Fab crystallization and permit IgE-Fc complex crystallization.,Mitropoulou AN, Ceska T, Heads JT, Beavil AJ, Henry AJ, McDonnell JM, Sutton BJ, Davies AM Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):116-129. doi:, 10.1107/S2053230X20001466. Epub 2020 Mar 2. PMID:32133997<ref>PMID:32133997</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6tco" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Beavil, A J]]
		+	[[Category: Ceska, T]]
		+	[[Category: Davies, A M]]
		+	[[Category: Henry, A J]]
		+	[[Category: McDonnell, J M]]
		+	[[Category: Mitropoulou, A N]]
		+	[[Category: Sutton, B J]]
		+	[[Category: Antibody]]
		+	[[Category: Fab]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]

OCA at 06:22, 4 December 2019

OCA — Wed, 04 Dec 2019 06:22:46 GMT

←Older revision		Revision as of 06:22, 4 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tco is ON HOLD	+	The entry 6tco is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6tco" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2019 09:29:16 GMT

Protected "6tco" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:29, 13 November 2019

OCA: New page: '''Unreleased structure''' The entry 6tco is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Nov 2019 09:29:14 GMT

New page: '''Unreleased structure''' The entry 6tco is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tco is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]