6tfq - Revision history

OCA at 07:38, 19 February 2020

OCA — Wed, 19 Feb 2020 07:38:06 GMT

←Older revision		Revision as of 07:38, 19 February 2020
Line 3:		Line 3:
	<StructureSection load='6tfq' size='340' side='right'caption='[[6tfq]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='6tfq' size='340' side='right'caption='[[6tfq]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tfq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TFQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tfq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TFQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=N72:(2~{R})-2-[[(3~{R},4~{R},5~{S})-3,4,5,6-tetrakis(oxidanyl)-2-oxidanylidene-hexyl]amino]pentanedioic+acid'>N72</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=N72:(2~{R})-2-[[(3~{R},4~{R},5~{S})-3,4,5,6-tetrakis(oxidanyl)-2-oxidanylidene-hexyl]amino]pentanedioic+acid'>N72</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">moaA, ddpA, A6U90_18755, At1D1609_52430, AtA6_55190 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=358 "Achromobacter radiobacter" (Beijerinck and van Delden 1902) Bergey et al. 1934])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tfq OCA], [http://pdbe.org/6tfq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tfq RCSB], [http://www.ebi.ac.uk/pdbsum/6tfq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tfq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tfq OCA], [http://pdbe.org/6tfq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tfq RCSB], [http://www.ebi.ac.uk/pdbsum/6tfq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tfq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 16:20, 22 January 2020

OCA — Wed, 22 Jan 2020 16:20:08 GMT

←Older revision		Revision as of 16:20, 22 January 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6tfq is ~~ON HOLD until Nov 14 2021~~	+	==Structure in P3212 form of the PBP/SBP MoaA in complex with mannopinic acid from A.tumefacien R10==
		+	<StructureSection load='6tfq' size='340' side='right'caption='[[6tfq]], [[Resolution\|resolution]] 2.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6tfq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TFQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=N72:(2~{R})-2-[[(3~{R},4~{R},5~{S})-3,4,5,6-tetrakis(oxidanyl)-2-oxidanylidene-hexyl]amino]pentanedioic+acid'>N72</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tfq OCA], [http://pdbe.org/6tfq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tfq RCSB], [http://www.ebi.ac.uk/pdbsum/6tfq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tfq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Agrobacterium tumefaciens pathogens use specific compounds denoted opines as nutrients in their plant tumor niche. These opines are produced by the host plant cells genetically modified by agrobacteria. They are imported into bacteria via solute-binding proteins (SBPs) in association with ABC transporters. The mannityl-opine family encompasses mannopine, mannopinic acid, agropine and agropinic acid. Structural and affinity data on mannopinic acid bound to SBPs are currently lacking while those of the three others mannityl opines are available. We investigated the molecular basis of two pathways for mannopinic acid uptake. MoaA was proposed as the specific SBP for mannopinic acid import in mannityl opines-assimilating agrobacteria, which was validated here using genetic studies and affinity measurements. We structurally characterized the mannopinic acid binding mode of MoaA in two crystal forms at 2.05 and 1.57 A resolution. We demonstrated that the non-specific SBP MotA, so far characterized as mannopine and Amadori compound importer, was also able to transport mannopinic acid. The structure of MotA bound to mannopinic acid at 2.2 A resolution defines a different mannopinic acid binding signature, similar to that of mannopine. Combining in vitro and in vivo approaches, this work allowed us to complete the characterization of the mannityl-opines assimilation pathways, highlighting the important role of two dual imports of agropinic and mannopinic acids. Our data shed new light on how the mannityl-opines contribute to the establishment of the ecological niche of agrobacteria from the early to the late stages of tumor development.

-	~~Authors~~:	+	Import pathways of the mannityl-opines into the bacterial pathogen Agrobacterium tumefaciens: structural, affinity and in vivo approaches.,Vigouroux A, Dore J, Marty L, Aumont-Nicaise M, Legrand P, Dessaux Y, Vial L, Morera S Biochem J. 2020 Jan 10. pii: 221837. doi: 10.1042/BCJ20190886. PMID:31922182<ref>PMID:31922182</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6tfq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Morera, S]]
		+	[[Category: Vigouroux, A]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Solute binding protein]]
		+	[[Category: Transport protein]]

OCA at 05:12, 27 November 2019

OCA — Wed, 27 Nov 2019 05:12:59 GMT

←Older revision		Revision as of 05:12, 27 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tfq is ON HOLD	+	The entry 6tfq is ON HOLD until Nov 14 2021

	Authors:		Authors:

OCA: Protected "6tfq" [edit=sysop:move=sysop]

OCA — Wed, 20 Nov 2019 13:43:28 GMT

Protected "6tfq" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:43, 20 November 2019

OCA: New page: '''Unreleased structure''' The entry 6tfq is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Nov 2019 13:43:27 GMT

New page: '''Unreleased structure''' The entry 6tfq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tfq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]