6tgn - Revision history

OCA at 10:18, 22 May 2024

OCA — Wed, 22 May 2024 10:18:10 GMT

←Older revision		Revision as of 10:18, 22 May 2024
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	<SX load='6tgn' size='340' side='right' viewer='molstar' caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6tgn' size='340' side='right' viewer='molstar' caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgn OCA], [~~http~~://pdbe.org/6tgn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tgn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tgn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgn OCA], [https://pdbe.org/6tgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tgn RCSB], [https://www.ebi.ac.uk/pdbsum/6tgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NBR1_ARATH NBR1_ARATH]] Autophagic substrate degraded in the vacuole by non-selective autophagy. Requires ATG8 protein expression to be recognized as an autophagic substrate (PubMed:21606687). Acts probably as a receptor for autophagosomal degradation of ubiquitinated proteins. Targets ubiquitinated protein aggregates derived from denatured or damaged non-native proteins generated under stress conditions (PubMed:23341779). Functions additively with the E3 ubiquitin-protein ligase CHIP for autophagosomal degradation of proteotoxic aggregates formed under stress conditions (PubMed:24497840).<ref>PMID:21606687</ref> <ref>PMID:23341779</ref> <ref>PMID:24497840</ref>	+	[https://www.uniprot.org/uniprot/NBR1_ARATH NBR1_ARATH] Autophagic substrate degraded in the vacuole by non-selective autophagy. Requires ATG8 protein expression to be recognized as an autophagic substrate (PubMed:21606687). Acts probably as a receptor for autophagosomal degradation of ubiquitinated proteins. Targets ubiquitinated protein aggregates derived from denatured or damaged non-native proteins generated under stress conditions (PubMed:23341779). Functions additively with the E3 ubiquitin-protein ligase CHIP for autophagosomal degradation of proteotoxic aggregates formed under stress conditions (PubMed:24497840).<ref>PMID:21606687</ref> <ref>PMID:23341779</ref> <ref>PMID:24497840</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jakobi~~, A J~~]]	+	[[Category: Jakobi AJ]]
-	[[Category: Sachse, C]]	+	[[Category: Sachse C]]
-	~~[[Category: Autophagy]]~~	+
-	~~[[Category: Helical filament]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:30, 11 April 2020

OCA — Sat, 11 Apr 2020 06:30:04 GMT

←Older revision		Revision as of 06:30, 11 April 2020
Line 3:		Line 3:
	<SX load='6tgn' size='340' side='right' viewer='molstar' caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6tgn' size='340' side='right' viewer='molstar' caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6TGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgn OCA], [http://pdbe.org/6tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tgn RCSB], [http://www.ebi.ac.uk/pdbsum/6tgn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgn ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgn OCA], [http://pdbe.org/6tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tgn RCSB], [http://www.ebi.ac.uk/pdbsum/6tgn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:00, 7 March 2020

OCA — Sat, 07 Mar 2020 01:00:37 GMT

←Older revision		Revision as of 01:00, 7 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of AtNBR1-PB1 filament (L-type)==		==Cryo-EM structure of AtNBR1-PB1 filament (L-type)==
-	<~~StructureSection~~ load='6tgn' size='340' side='right'caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>	+	<SX load='6tgn' size='340' side='right' viewer='molstar' caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TGN FirstGlance]. <br>		<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TGN FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jakobi, A J]]		[[Category: Jakobi, A J]]

OCA at 04:25, 13 February 2020

OCA — Thu, 13 Feb 2020 04:25:25 GMT

←Older revision		Revision as of 04:25, 13 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6tgn is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of AtNBR1-PB1 filament (L-type)==
		+	<StructureSection load='6tgn' size='340' side='right'caption='[[6tgn]], [[Resolution\|resolution]] 4.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6tgn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TGN FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgn OCA], [http://pdbe.org/6tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tgn RCSB], [http://www.ebi.ac.uk/pdbsum/6tgn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NBR1_ARATH NBR1_ARATH]] Autophagic substrate degraded in the vacuole by non-selective autophagy. Requires ATG8 protein expression to be recognized as an autophagic substrate (PubMed:21606687). Acts probably as a receptor for autophagosomal degradation of ubiquitinated proteins. Targets ubiquitinated protein aggregates derived from denatured or damaged non-native proteins generated under stress conditions (PubMed:23341779). Functions additively with the E3 ubiquitin-protein ligase CHIP for autophagosomal degradation of proteotoxic aggregates formed under stress conditions (PubMed:24497840).<ref>PMID:21606687</ref> <ref>PMID:23341779</ref> <ref>PMID:24497840</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	p62/SQSTM1 is an autophagy receptor and signaling adaptor with an N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in the cell. The molecular determinants that govern PB1 domain filament formation in vitro remain to be determined and the role of p62 filaments inside the cell is currently unclear. We here determine four high-resolution cryo-EM structures of different human and Arabidopsis PB1 domain assemblies and observed a filamentous ultrastructure of p62/SQSTM1 bodies using correlative cellular EM. We show that oligomerization or polymerization, driven by a double arginine finger in the PB1 domain, is a general requirement for lysosomal targeting of p62. Furthermore, the filamentous assembly state of p62 is required for autophagosomal processing of the p62-specific cargo KEAP1. Our results show that using such mechanisms, p62 filaments can be critical for cargo uptake in autophagy and are an integral part of phase-separated p62 bodies.

-	~~Authors:~~ Jakobi, A~~.J.~~, ~~Sachse~~, C.	+	Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake.,Jakobi AJ, Huber ST, Mortensen SA, Schultz SW, Palara A, Kuhm T, Shrestha BK, Lamark T, Hagen WJH, Wilmanns M, Johansen T, Brech A, Sachse C Nat Commun. 2020 Jan 23;11(1):440. doi: 10.1038/s41467-020-14343-8. PMID:31974402<ref>PMID:31974402</ref>

-	~~Description: Cryo-EM structure~~ of ~~AtNBR1~~-~~PB1 filament (L~~-~~type)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Jakobi, A.J]]	+	<div class="pdbe-citations 6tgn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jakobi, A J]]
	[[Category: Sachse, C]]		[[Category: Sachse, C]]
		+	[[Category: Autophagy]]
		+	[[Category: Helical filament]]
		+	[[Category: Signaling protein]]

OCA at 13:15, 29 January 2020

OCA — Wed, 29 Jan 2020 13:15:58 GMT

←Older revision		Revision as of 13:15, 29 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tgn is ON HOLD until ~~sometime in the future~~	+	The entry 6tgn is ON HOLD until Paper Publication

	Authors: Jakobi, A.J., Sachse, C.		Authors: Jakobi, A.J., Sachse, C.

OCA: Protected "6tgn" [edit=sysop:move=sysop]

OCA — Wed, 27 Nov 2019 05:15:04 GMT

Protected "6tgn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 27 November 2019

OCA: New page: '''Unreleased structure''' The entry 6tgn is ON HOLD until sometime in the future Authors: Jakobi, A.J., Sachse, C. Description: Cryo-EM structure of AtNBR1-PB1 filament (L-type) [[Cat...

OCA — Wed, 27 Nov 2019 05:15:02 GMT

New page: '''Unreleased structure''' The entry 6tgn is ON HOLD until sometime in the future Authors: Jakobi, A.J., Sachse, C. Description: Cryo-EM structure of AtNBR1-PB1 filament (L-type) [[Cat...

New page

'''Unreleased structure'''

The entry 6tgn is ON HOLD until sometime in the future

Authors: Jakobi, A.J., Sachse, C.

Description: Cryo-EM structure of AtNBR1-PB1 filament (L-type)
[[Category: Unreleased Structures]]
[[Category: Jakobi, A.J]]
[[Category: Sachse, C]]