6tit - Revision history

OCA at 13:03, 24 January 2024

OCA — Wed, 24 Jan 2024 13:03:04 GMT

←Older revision		Revision as of 13:03, 24 January 2024
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	<StructureSection load='6tit' size='340' side='right'caption='[[6tit]], [[Resolution\|resolution]] 2.07Å' scene=''>		<StructureSection load='6tit' size='340' side='right'caption='[[6tit]], [[Resolution\|resolution]] 2.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Recombinant_vesicular_stomatitis_indiana_virus_rvsv~~-g/~~gfp~~ Recombinant vesicular stomatitis ~~indiana~~ virus ~~rvsv~~-g/~~gfp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Recombinant_vesicular_stomatitis_Indiana_virus_rVSV-G/GFP Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.07Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tit OCA], [https://pdbe.org/6tit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tit RCSB], [https://www.ebi.ac.uk/pdbsum/6tit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tit OCA], [https://pdbe.org/6tit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tit RCSB], [https://www.ebi.ac.uk/pdbsum/6tit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tit ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B7UCZ5_9RHAB B7UCZ5_9RHAB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Recombinant vesicular stomatitis ~~indiana~~ virus ~~rvsv~~-g/~~gfp~~]]	+	[[Category: Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP]]
-	[[Category: Abouhamdan, A]]	+	[[Category: Abouhamdan A]]
-	[[Category: Albertini~~, A A~~]]	+	[[Category: Albertini AA]]
-	[[Category: Belot, L]]	+	[[Category: Belot L]]
-	[[Category: Gaudin, Y]]	+	[[Category: Gaudin Y]]
-	~~[[Category: Ectodomain]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Vesicular stomatitis virus]]~~	+
-	~~[[Category: Viral fusion protein]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Vsv~~]]	+

OCA at 06:52, 17 March 2021

OCA — Wed, 17 Mar 2021 06:52:31 GMT

←Older revision		Revision as of 06:52, 17 March 2021
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	==VSV G_440==		==VSV G_440==
-	<StructureSection load='6tit' size='340' side='right'caption='[[6tit]]' scene=''>	+	<StructureSection load='6tit' size='340' side='right'caption='[[6tit]], [[Resolution\|resolution]] 2.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Recombinant_vesicular_stomatitis_indiana_virus_rvsv-g/gfp Recombinant vesicular stomatitis indiana virus rvsv-g/gfp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tit OCA], [~~http~~://pdbe.org/6tit PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tit RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tit PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tit ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tit OCA], [https://pdbe.org/6tit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tit RCSB], [https://www.ebi.ac.uk/pdbsum/6tit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tit ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	VSV fusion machinery, like that of many other enveloped viruses, is triggered at low pH in endosomes after virion endocytosis. It was suggested that some histidines could play the role of pH-sensitive switches. By mutating histidine residues H22, H60, H132, H162, H389, H397, H407, and H409, we demonstrate that residues H389 and D280, facing each other in the six-helix bundle of the post-fusion state, and more prominently H407, located at the interface between the C-terminal part of the ectodomain and the fusion domain, are crucial for fusion. Passages of recombinant viruses bearing mutant G resulted in the selection of compensatory mutations. Thus, the H407A mutation in G resulted in two independent compensatory mutants, L396I and S422I. Together with a crystal structure of G, presented here, which extends our knowledge of G pre-fusion structure, this indicates that the conformational transition is initiated by refolding of the C-terminal part of the G ectodomain.
		+
		+	Identification of a pH-Sensitive Switch in VSV-G and a Crystal Structure of the G Pre-fusion State Highlight the VSV-G Structural Transition Pathway.,Beilstein F, Abou Hamdan A, Raux H, Belot L, Ouldali M, Albertini AA, Gaudin Y Cell Rep. 2020 Aug 18;32(7):108042. doi: 10.1016/j.celrep.2020.108042. PMID:32814045<ref>PMID:32814045</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tit" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abouhamdan A]]	+	[[Category: Recombinant vesicular stomatitis indiana virus rvsv-g/gfp]]
-	[[Category: Albertini AA]]	+	[[Category: Abouhamdan, A]]
-	[[Category: Belot L]]	+	[[Category: Albertini, A A]]
-	[[Category: Gaudin Y]]	+	[[Category: Belot, L]]
		+	[[Category: Gaudin, Y]]
		+	[[Category: Ectodomain]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Vesicular stomatitis virus]]
		+	[[Category: Viral fusion protein]]
		+	[[Category: Viral protein]]
		+	[[Category: Vsv]]

OCA at 06:48, 2 September 2020

OCA — Wed, 02 Sep 2020 06:48:27 GMT

←Older revision		Revision as of 06:48, 2 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6tit ~~is ON HOLD until Paper Publication~~	+	==VSV G_440==
-		+	<StructureSection load='6tit' size='340' side='right'caption='[[6tit]]' scene=''>
-	~~Authors~~: ~~Albertini~~, A.A., ~~Belot~~, L., ~~Abouhamdan, A~~., ~~Gaudin, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TIT FirstGlance]. <br>
-	~~Description: VSV G_440~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tit OCA], [http://pdbe.org/6tit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tit RCSB], [http://www.ebi.ac.uk/pdbsum/6tit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tit ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Abouhamdan, A]]	+	__TOC__
-	[[Category: Albertini~~, A.A~~]]	+	</StructureSection>
-	[[Category: Belot, L]]	+	[[Category: Large Structures]]
-	[[Category: Gaudin, Y]]	+	[[Category: Abouhamdan A]]
		+	[[Category: Albertini AA]]
		+	[[Category: Belot L]]
		+	[[Category: Gaudin Y]]

OCA at 06:16, 22 July 2020

OCA — Wed, 22 Jul 2020 06:16:23 GMT

←Older revision		Revision as of 06:16, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tit is ON HOLD	+	The entry 6tit is ON HOLD until Paper Publication

	Authors: Albertini, A.A., Belot, L., Abouhamdan, A., Gaudin, Y.		Authors: Albertini, A.A., Belot, L., Abouhamdan, A., Gaudin, Y.

OCA: Protected "6tit" [edit=sysop:move=sysop]

OCA — Wed, 04 Dec 2019 06:25:14 GMT

Protected "6tit" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 4 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tit is ON HOLD Authors: Albertini, A.A., Belot, L., Abouhamdan, A., Gaudin, Y. Description: VSV G_440 Category: Unreleased Structures [[Catego...

OCA — Wed, 04 Dec 2019 06:25:13 GMT

New page: '''Unreleased structure''' The entry 6tit is ON HOLD Authors: Albertini, A.A., Belot, L., Abouhamdan, A., Gaudin, Y. Description: VSV G_440 Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 6tit is ON HOLD

Authors: Albertini, A.A., Belot, L., Abouhamdan, A., Gaudin, Y.

Description: VSV G_440
[[Category: Unreleased Structures]]
[[Category: Abouhamdan, A]]
[[Category: Albertini, A.A]]
[[Category: Belot, L]]
[[Category: Gaudin, Y]]