OCA at 13:03, 24 January 2024

OCA — Wed, 24 Jan 2024 13:03:06 GMT

←Older revision		Revision as of 13:03, 24 January 2024
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-	====	+	==DROSOPHILA GTP-TUBULIN Y222F MUTANT==
-	<StructureSection load='6tiu' size='340' side='right'caption='[[6tiu]]' scene=''>	+	<StructureSection load='6tiu' size='340' side='right'caption='[[6tiu]], [[Resolution\|resolution]] 3.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tiu]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tiu OCA], [https://pdbe.org/6tiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tiu RCSB], [https://www.ebi.ac.uk/pdbsum/6tiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tiu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.571Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tiu OCA], [https://pdbe.org/6tiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tiu RCSB], [https://www.ebi.ac.uk/pdbsum/6tiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tiu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TBA1_DROME TBA1_DROME] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nucleation of microtubules (MTs) is essential for cellular activities, but its mechanism is unknown because of the difficulty involved in capturing rare stochastic events in the early stage of polymerization. Here, combining rapid flush negative stain electron microscopy (EM) and kinetic analysis, we demonstrate that the formation of straight oligomers of critical size is essential for nucleation. Both GDP and GTP tubulin form single-stranded oligomers with a broad range of curvatures, but upon nucleation, the curvature distribution of GTP oligomers is shifted to produce a minor population of straight oligomers. With tubulin having the Y222F mutation in the beta subunit, the proportion of straight oligomers increases and nucleation accelerates. Our results support a model in which GTP binding generates a minor population of straight oligomers compatible with lateral association and further growth to MTs. This study suggests that cellular factors involved in nucleation promote it via stabilization of straight oligomers.
		+
		+	GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.,Ayukawa R, Iwata S, Imai H, Kamimura S, Hayashi M, Ngo KX, Minoura I, Uchimura S, Makino T, Shirouzu M, Shigematsu H, Sekimoto K, Gigant B, Muto E J Cell Biol. 2021 Apr 5;220(4):e202007033. doi: 10.1083/jcb.202007033. PMID:33544140<ref>PMID:33544140</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tiu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Stathmin-4 3D structures\|Stathmin-4 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Rattus norvegicus]]
		+	[[Category: Gigant B]]

OCA at 07:53, 27 January 2021

OCA — Wed, 27 Jan 2021 07:53:12 GMT

←Older revision		Revision as of 07:53, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6tiu ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6tiu' size='340' side='right'caption='[[6tiu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tiu OCA], [https://pdbe.org/6tiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tiu RCSB], [https://www.ebi.ac.uk/pdbsum/6tiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tiu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "6tiu" [edit=sysop:move=sysop]

OCA — Wed, 04 Dec 2019 06:25:27 GMT

Protected "6tiu" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 4 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tiu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Dec 2019 06:25:25 GMT

New page: '''Unreleased structure''' The entry 6tiu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tiu is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6tiu - Revision history

OCA at 13:03, 24 January 2024

OCA at 07:53, 27 January 2021

OCA: Protected "6tiu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6tiu is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures